Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Die-Back on Rose (Rosa hybrida) Caused by Lasiodiplodia pseudotheobromae in Korea

Lasiodiplodia pseudotheobromae에 의한 장미 가지썩음병의 발생 보고

  • Wee, Jung-In (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Park, Mi-Jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Chang, Taehyun (School of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University) ;
  • Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science)
  • 위정인 (국립원예특작과학원 원예특작환경과) ;
  • 백창기 (국립원예특작과학원 원예특작환경과) ;
  • 박미정 (국립원예특작과학원 원예특작환경과) ;
  • 장태현 (경북대학교 생태환경시스템학과) ;
  • 박종한 (국립원예특작과학원 원예특작환경과)
  • Received : 2017.08.10
  • Accepted : 2017.09.19
  • Published : 2017.12.31
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Abstract

In 2015, symptoms of die-back on Rosa hybrida were observed in Taean, Korea. The aims of this study were to determine the cause of die-back on Rosa hybrida and characterize the pathogen. The fungal isolates were obtained and used for pathogenicity test, morphological and molecular analyses. The pathogenicity test on healthy branches of Rosa hybrida produced die-back, as the original symptoms. For the morphological study, the isolates were inoculated onto potato dextrose agar and incubated for 7 days at $25^{\circ}C$. The colonies grew up quickly and turned white to gray in color. Conidia were observed under an optical microscope. The features of conidia were ellipsoidal, grayish brown in color, $20-31{\times}11-17{\mu}m$ in size and had one septum. Molecular analyses of the ITS region, TEF and TUB genes were conducted to confirm the identity of the pathogen. The phylogenetic tree of the multi-gene sequences indicated that the causal agent was Lasiodiplodia pseudotheobromae. This study is the first report of die-back caused by Lasiodiplodia pseudotheobromae on Rose (Rosa hybrida).

2015년에 태안에서 장미의 가지 썩음 증상과 검은 포자체가 붙어 있는 새로운 증상이 관찰되었다. 이 증상의 원인을 찾기 위해 장미 가지의 썩음 증상에서 균사체를 분리하였다. 분리 균주의 병원성을 검정하였더니 건전한 장미 가지가 썩었으며 처음 발견했던 병징과 일치하였다. 형태학적 특성을 조사하고 분자생물학적 분석을 위하여 병원균을 $25^{\circ}C$에 7일간 배양하였다. 병원균의 균사 생장은 빠르며 균총의 색깔은 흰색에서 잿빛으로 변했다. 광학현미경으로 관찰한 분생포자는 회갈색의 타원모양에 격막이 하나 있으며 크기는 $20-31{\times}11-17{\mu}m$이다. 병원균의 ITS 영역, TEF와 TUB 유전자의 염기서열을 결합하여 근연종과 유연관계를 분석한 결과 Lasiodiplodia pseudotheobromae로 동정되었다. 이에 따라 장미에서 L. pseudotheobromae이 발생시키는 가지 썩음 증상을 장미 가지썩음병으로 명명하여 보고하고자 한다.

Keywords

References

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