Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection

  • Choi, In Young (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Ju-Hoon (Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Hye-jin (Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University) ;
  • Park, Mi-Kyung (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2017.11.08
  • Accepted : 2017.11.16
  • Published : 2017.12.28
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Abstract

Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is highly stable under various pH (5-11), temperature ($4-60^{\circ}C$), and organic solvent conditions. The KFS-SE1 genome consisted of 59,715 bp with 73 predicted open reading frames and 57.14% GC content; it had a complete set of genes required for phage reconstruction. Comparative phylogenetic analysis of KFS-SE1 revealed that it was very similar to the other Salmonella phages in the Siphoviridae family. These characteristics suggest that KFS-SE1 with its high specificity and host lysis activity toward S. Enteritidis may have various potential applications.

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