Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Bioethanol Production from the Red Seaweed Eucheuma denticulatum

홍조류(Eucheuma denticulatum)를 이용한 바이오에탄올 생산

  • Kim, Sang Won (Department of Biotechnology, Pukyong National University) ;
  • Gwak, Seung Hee (Department of Biotechnology, Pukyong National University) ;
  • Ra, Chae Hun (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
  • Received : 2017.10.16
  • Accepted : 2017.12.12
  • Published : 2017.12.28
Download PDF
⟨ Previous Next ⟩

Abstract

Hyperthermal acid hydrolysis pretreatment of Eucheuma denticulatum was carried out using 12% (w/v) seaweed slurry and 90 mM $H_3PO_4$ at $150^{\circ}C$ for 10 min. The use of Candida lusitaniae with adaptive evolution was evaluated for ethanol fermentation. The levels of ethanol production by separate hydrolysis and fermentation (SHF) at 72 h with non-adapted and adapted C. lusitaniae were 10.1 g/l with ethanol yield ($Y_{EtOH}$) of 0.23, and 18.1 g/l with $Y_{EtOH}$ of 0.45, respectively. Adaptive evolution was employed in this study to improve the efficiency of ethanol fermentation. Development of the SHF process could enhance the overall ethanol fermentation yields of the red seaweed E. denticulatum.

Keywords

References

  1. Bothast RJ, Schlicher MA. 2005. Biotechnological processes for conversion of corn into ethanol. Appl. Microbiol. Biotechnol. 67: 19-25. https://doi.org/10.1007/s00253-004-1819-8
  2. Dias MOS, Esinas AV, Nebra SA, Filho RM, Rossel CEV, Maciel MRW. 2009. Production of bioethanol and other bio-based materials from sugarcane bagasse: integration to conventional bioethanol production process. Chem. Eng. Res. 87: 1206-1216. https://doi.org/10.1016/j.cherd.2009.06.020
  3. Chiaramonti D, Prussi M, Ferrero S, Oriani L, Ottonello P, Torre P, et al. 2012. Review of pretreatment processes for lignocellulosic ethanol production. Biomass. Bioenergy 46: 25-35. https://doi.org/10.1016/j.biombioe.2012.04.020
  4. Tong HK, Lee KH, Wong HA. 2007. Study of the water extractable components of the red seaweed Eucheuma spinosum. Int. J. Food Sci. Technol. 14: 265-275. https://doi.org/10.1111/j.1365-2621.1979.tb00870.x
  5. Lin L, Tako M, Hongo F. 2000. Isolation and characterization of I-carrageenan from Eucheuma serra (Togekirinsai). J. Appl. Glycosci. 47: 303-310. https://doi.org/10.5458/jag.47.303
  6. Sanchez-Machado DI, Lopez-Cervantes J, Paseiro-Losada P, Lopez-Hernandez J. 2004. Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chem. 85: 439-444. https://doi.org/10.1016/j.foodchem.2003.08.001
  7. Cho HY, Ra CH, Kim SK. 2014. Ethanol production from the seaweed, Gelidium amansii using specific sugar acclimated yeasts. J. Microbiol. Biotechnol. 24: 264-269. https://doi.org/10.4014/jmb.1307.07054
  8. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428. https://doi.org/10.1021/ac60147a030
  9. Nguyen TH, Ra CH, Park MR, Jeong GT, Kim SK. 2016. Bioethanol production from seaweed Undaria pinnatipida using various yeasts by Separate Hydrolysis and Fermentation (SHF). Microbiol. Biotechnol. Lett. 44: 529-534. https://doi.org/10.4014/mbl.1610.10007
  10. Qi L, Mui YF, Lo SW, Lui MY, Akien GR, Horvath IT. 2014. Catalytic conversion of fructose, glucose, and sucrose to 5-(hydroxymethyl) furfural and levulinic and formic acids in ${\gamma}$-valerolactone as a green solvent. ACS Catal. 4: 1470-1477. https://doi.org/10.1021/cs401160y
  11. Ahn DJ, Kim SK, Yun HS. 2012. Optimization of pretreatment and saccharification for the production of bioethanol from water hyacinth by Saccharomyces cerevisiae. Bioprocess Biosyst. Eng. 35: 35-41. https://doi.org/10.1007/s00449-011-0600-5
  12. Lee SM, Lee JH. 2012. Ethanol fermentation for main sugar components of brown-algae using various yeasts. J. Ind. Eng. Chem. 18: 16-18. https://doi.org/10.1016/j.jiec.2011.11.097
  13. Tan IS, Lee KT. 2015. Solid acid catalysts pretreatment and enzymatic hydrolysis of macroalgae cellulosic residue for the production of bioethanol. Carbohydr. Polym. 124: 311-321. https://doi.org/10.1016/j.carbpol.2015.02.046