Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Mixture Lactic Acid Bacteria and Sea Tangle on Anti-obesity and Gut Microflora in Rats

흰 쥐에서 복합 유산균과 다시마가 항비만 및 장내 미생물에 미치는 영향

  • Yu, Da Yoon (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, Jeong A (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, In Sung (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Lee, Chul Young (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, Seong chan (Colleges of Medicine, Hallym University) ;
  • Lee, Sang Suk (Department of Animal Science and Technology, Sunchon National University) ;
  • Choi, In Soon (Department of Life Science, Silla University) ;
  • Cho, Kwang Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
  • 유다윤 (경남과학기술대학교 동물소재공학과) ;
  • 김정아 (경남과학기술대학교 동물소재공학과) ;
  • 김인성 (경남과학기술대학교 동물소재공학과) ;
  • 이철영 (경남과학기술대학교 동물소재공학과) ;
  • 김성찬 (한림대학교 의과대학) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 최인순 (신라대학교 생명과학과) ;
  • 조광근 (경남과학기술대학교 동물소재공학과)
  • Received : 2017.11.14
  • Accepted : 2017.12.19
  • Published : 2017.12.30
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Abstract

The present study was undertaken to investigate the effects of dietary provision of lactic acid bacteria (LB) and sea tangle (ST) on the obesity-associated intestinal microbiota in rats with obesity induced by a high-fat diet. Forty-eight 8-wk-old Sprague-Dawley rats were fed a basal diet (CON), a high fat diet (HFD; CON supplemented with 10% lard), HF supplemented with LB [HFL; $5{\times}10^8cfu$ of each of Lactobacillus rhamnosus, Lactobacillus johnsonii, Bifidobacterium longum and Bifidobacterium lactis], or HFL containing 10% ST (HFLS), with 4 replicates (cages) of 3 rats per dietary treatment, for 6 wk, and the intestinal microbiota were determined by pyrosequencing. The HFL and HFLS groups exhibited reduced rates of weight gain than the HF group, and the former groups had smaller ratios of Firmicutes and greater ratios of Bacteriodetes, with decreased Firmicutes/Bacteroidetes ratios, than the latter at the level of the phylum. Compared with the results for the HF group, HFL and HFLS had reduced ratios of the families of Roseburia, Mollicute, Erysipelotrichi, and Oscillibacter within Firmicutes associated with obesity and increased ratios of the families of Prevotella, Alistipes and Bacteroides within the Bacterioidetes phylum known to have an anti-obesity effect. The content of butyric acid in feces was greater in the HFLS group vs. HF and HFL. In conclusion, the present results suggest that dietary provision of LB plus ST has an anti-obesity effect and induced changes in intestinal microorganisms, and enhanced the content of butyric acid, which is an intestinal metabolite.

본 연구에서는 유산균과 다시마가 항비만과 장내 미생물에 미치는 영향을 조사하기 위하여 8주령의 수컷 흰쥐를 1 cage 당 3 마리씩 4반복하여 처리군 당 12마리를 사용하였으며, 대조구 기초사료에 고지방사료 처리구는 10% lard를 첨가하고, 여기에 5% 유산균($5{\times}10^8cfu$, Lactobacillus rhamnosus, Lactobacillus johnsonii, Bifidobacterium longum, Bifidobacterium lactis) 첨가구, 5% 유산균과 10% 다시마를 혼합 첨가한 처리구로 구분하였다. 고지방사료 처리군에 비해 유산균 처리군과 다시마와 유산균 혼합 처리군은 체중과 일당 증체량의 감소를 나타내었다. 장내미생물을 pyrosequncing 방법으로 확인한 결과, 고지방사료 처리군에 비해 유산균 처리군과 다시마와 유산균 혼합 처리군은 Firmicutes 비율이 감소하고, Bacteroidetes의 비율이 증가하였으며, Firmicutes/Bacteroidetes (F/B) 비율은 감소하였다. 또한 이 두 처리군은 Firmicutes 문에 속하는 비만 관련 미생물 Roseburia, Mollicute, Erysipelotrichi, Oscillibacter가 감소하였고, 반면에 Bacteroidetes 문에 속하는 항비만 관련 미생물 Prevotella, Alistipes, Bacteroides는 증가하였다. 분변 내 단쇄지방산(short chain fatty acid; SCFA) 은 다시마와 유산균을 섭취한 처리군에서 butyric acid 함량이 높게 나타났다. 결론적으로, 다시마와 유산균의 혼합 처리는 항비만 효과를 나타내며 장내 미생물 변화를 유도하고 장내 대사 물질인 butyric acid의 함량을 높이는 것을 시사한다.

Keywords

References

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