한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제25권6호
  • /
  • Pages.367-374
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  • 2017
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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지방분화가 유도된 3T3-L1 세포와 고지방식이로 유도된 마우스에서 보리순 물추출물의 항비만 효과

Anti-obesity Effects of Barley Sprout Young Leaf on 3T3-L1 Cells and High-fat Diet-induced Obese Mice

  • 강병만 (한약진흥재단 한약자원본부) ;
  • 심미옥 (한약진흥재단 한약자원본부) ;
  • 김민석 (한약진흥재단 한약자원본부) ;
  • 유승진 ((주)예다손) ;
  • 여준환 (한약진흥재단 한약자원본부) ;
  • 정원석 (한약진흥재단 한약자원본부)
  • Kang, Byoung Man (Division of Traditional Korean Medicine Resource, NIKOM,) ;
  • Sim, Mi Ok (Division of Traditional Korean Medicine Resource, NIKOM,) ;
  • Kim, Min Suk (Division of Traditional Korean Medicine Resource, NIKOM,) ;
  • Yoo, Seung Jin (Yedason Co., Ltd.) ;
  • Yeo, Jun Hwan (Division of Traditional Korean Medicine Resource, NIKOM,) ;
  • Jung, Won Seok (Division of Traditional Korean Medicine Resource, NIKOM,)
  • 투고 : 2017.09.12
  • 심사 : 2017.11.22
  • 발행 : 2017.12.30
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초록

Background: An imbalance in energy intake and expenditure can cause obesity, which is a major risk factor for chronic diseases such as heart disease, type 2 diabetes, insulin resistance, cancers and hyperlipidemia. Methods and Results: In this study, we evaluated the anti-obesity effects of a water extract from the young leaves of barley sprout (BS) in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice (HF). Lipid accumulation measurement indicates that BS markedly inhibited adipogenesis by reducing lipid droplet production in a dose-dependent manner. Furthermore, the mRNA expression of adipogenic transcription factors peroxisome proliferator-activated receptor-${\gamma}$ and fatty acid synthetase, CCAAT/enhancer binding protein-${\alpha}$ and fatty acid binding protein 4 in 3T3-L1 cells was significantly inhibited by BS treatment. In an in vivo test, the BS-administered group of HFD-induced mice showed less body weight gain, and lower liver and epididymal white adipose tissue weights. The BS-treated mice showed decreased serum levels of leptin and lipids compared to untreated HFD mice and the levels of adiponectin and the HDL-cholesterol/total cholesterol ratio increased. These results indicate that BS inhibits body fat accumulation by reducing the mRNA expression of lipogenesis transcription factors and increasing serum adipokine concentration in in vitro and in vivo tests. Conclusions: BS reduced high fat diet-induced weight gain and had a positive effect on dyslipidemia.

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참고문헌

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