Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Anti-scuticociliate effects of aquatic hydrogen peroxide preparation in olive flounder Paralichthys olivaceus

넙치에서 수산용 과산화수소의 항스쿠티카 효과

  • Lee, Ji-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Jung-Jin (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Choi, Jun-Ho (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Shin, Dong-Hun (Shindong Control Center For Aquatic Animal Diseases) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 이지훈 (군산대학교 수산생명의학과) ;
  • 박정진 (군산대학교 수산생명의학과) ;
  • 최준호 (군산대학교 수산생명의학과) ;
  • 신동훈 (신동수산질병관리원) ;
  • 박관하 (군산대학교 수산생명의학과)
  • Received : 2017.10.27
  • Accepted : 2017.11.14
  • Published : 2017.12.30
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Abstract

We evaluated the anti-parasitic efficacy of hydrogen peroxide against a scuticociliate Miamiensis avidus in olive flounder Paralichthys olivaceus. In vitro tests showed that hydrogen peroxide above 50 mg/l is effective for killing M. avidus. In vivo effects were assessed in olive flounder after immersion infection with $2.8{\times}10^3M.\;avidus/ml$ for 2 days, and in naturally infected fish obtained from Jeju area farms. Bathing treatment with 100 and 200 mg/l hydrogen peroxide showed markedly higher survival rates of olive flounder than control groups (p<0.05). The number of live M. avidus in fish tissues were reduced for 3 days in naturally-infected fish after a single bath. In conclusion, hydrogen peroxide can be a good therapeutant particularly in the early stage of M. avidus infection.

본 연구에서는 넙치 (Paralichthys olivaceus)에게 감염 및 높은 폐사율을 일으켜 해산어류양식에서 심각한 문제가 되고 있는 스쿠티카충 (Miamiensis avidus) 구제를 위한 과산화수소의 유효성을 평가하였다. In vitro시험 결과, 과산화수소 50 mg/l로 1시간 동안 노출시키면 완벽하게 스쿠티카충 (M. avidus)을 효과적으로 살상하였다. In vivo시험은 $2{\times}10^3M.\; avidus/ml$로 이틀간 침지감염을 유도한 넙치와 제주지역에서 자연적으로 스쿠티카충에 감염된 넙치를 대상으로 과산화수소의 효능을 평가하였다. 제주지역 양식장 및 실험실적 시험군에서 과산화수소 100-200 mg/l로 1시간 동안 약욕시 대조군에 비해 현저히 누적폐사가 감소하였다 (p<0.05). 이때 넙치 조직에서 충밀도 측정 결과 약욕 후 3일 동안 대조군에 비해 감소가 나타났다. 따라서 수산용 과산화수소는 스쿠티카충의 초기 구제 효과가 유효한 것으로 확인하였다.

Keywords

References

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