Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV

  • Quilantang, Norman G. (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Ki Ho (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Ju Sung (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, EunJu (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2017.09.04
  • Accepted : 2017.09.25
  • Published : 2017.12.29
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Abstract

A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water: acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO $C_{18}$ column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were $10{\mu}L$, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.

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References

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