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Development and characterization of eleven microsatellite markers for a popular pet stag beetle, Dorcus hopei (Coleoptera, Lucanidae) using paired-end Illumina shotgun sequencing

  • Han, Taeman (Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science) ;
  • Kim, Seung-Hyun (Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science) ;
  • Park, In Gyun (Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science) ;
  • Park, Haechul (Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science)
  • Received : 2017.09.12
  • Accepted : 2017.10.17
  • Published : 2017.12.31

Abstract

Eleven polymorphic microsatellite loci were developed and characterized for Dorcus hopei in this study. The number of alleles varied from 2 to 21. The observed heterozygosity and expected heterozygosity ranged from 0.1058 to 0.9744 and 0.0997 to 0.8941, respectively. Two loci showed low polymorphism, while the rest were highly polymorphic. Six loci deviated from Hardy-Weinberg Equilibrium. The set of markers will provide effective tools for examining the population genetic structures and be helpful for managing wild population in D. hopei.

Keywords

References

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