Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Growth Inhibition of Clostridium difficile by Fermented Broccoli with Leuconostoc mesenteroides

Leuconostoc mesenteroides를 이용한 브로콜리 발효물에 의한 Clostridium difficile의 생육 제어

  • Lee, Young-Duck (Department of Food Science and Engineering, Seowon University) ;
  • Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation)
  • 이영덕 (서원대학교 식품공학과) ;
  • 문기성 (한국교통대학교 생명공학과)
  • Received : 2017.10.27
  • Accepted : 2017.11.15
  • Published : 2017.12.30
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Abstract

In this study, Leuconostoc mesenteroides CJNU0041 was isolated from Korean traditional food kimchi and antimicrobial activity of fermented broccoli with the isolate was tested against pathogenic Clostridium difficile. L. mesenteroides CJNU0041 showed higher glucosidase activity than other isolates. As the results of physiological properties such as pH and viable cell count during broccoli fermentation with L. mesenteroides CJNU0041, we confirmed that 48 hours was optimal fermentation time. As the results of metabolite analysis by HPLC, metabolites were changed during the fermentation. Especially, the growth of C. difficile was inhibited by the fermented broccoli. Therefore, L. mesenteroides CJNU0041 might be a candidate for improving the functionality of natural materials by lactic acid fermentation.

본 연구에서는 김치로부터 L. mesenteroides CJNU0041을 분리하여 16S rRNA 염기서열 분석을 통해, 동정하였으며, ${\beta}$-glucosidase 활성이 우수한 것으로 나타났다. L. mesenteroides CJNU0041를 이용한 브로콜리 발효 동안 생균수와 pH 및 ${\beta}$-glucosidase 활성에 대해 분석한 결과, 발효 시간은 48 hours이 적당한 것으로 판단되었다. 또한, 발효 후 생물 전환이 일어남을 HPLC 분석을 통해 확인하였다. 그리고, L. mesenteroides CJNU0041의 브로콜리 발효물에서 C. difficile에 대한 생육 억제 효과를 확인 할 수 있었다. 따라서, 본 연구를 통해 분리된 신규 L. mesenteroides CJNU0041을 C. difficile의 생육 제어를 위한 유산균 제재화가 가능할 것으로 판단되며, 브로콜리 발효물도 다양한 식품에 적용이 가능할 것으로 사료된다.

Keywords

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