Journal of Applied Biological Chemistry

  • 제60권4호
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  • Pages.373-381
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  • 2017
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Effects of sea horse (Hippocampus abdominalis)-derived protein hydrolysate on skeletal muscle development

  • Muthuramalingam, Karthika (Department of Medicine, School of Medicine, Jeju National University) ;
  • Kim, Jun Ho (Department of Medicine, School of Medicine, Jeju National University) ;
  • Jeon, You Jin (Department of Marine Life Science, Jeju National University) ;
  • Rho, Sum (Haechunma Company Ltd) ;
  • Kim, Young Mee (Department of Medicine, School of Medicine, Jeju National University) ;
  • Cho, Moonjae (Department of Medicine, School of Medicine, Jeju National University)
  • 투고 : 2017.10.12
  • 심사 : 2017.11.07
  • 발행 : 2017.12.01
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초록

Hippocampus abdominalis, the big belly sea horse, is widely known for its medicinal value in Chinese folk medicine. In this study, extract obtained by proteolytic degradation of this species was investigated for its effects on skeletal muscle development, both in vitro and in vivo. Muscle cell lines ($C_2C_{12}$ and $L_6$) treated with the bioactive peptide did not have any detrimental effects on the cell viability, which was above 80%. Optical microscopy analysis on the morphology of the sea horse extract (SHE)-treated cells showed enhanced differentiating ability with myotube formation. Moreover, cells incubated with the hydrolysate displayed decreased proliferation rate, as recorded by the electric cell substrate impedance sensing system, thereby supporting enhanced differentiation. For a period of 12 weeks, mice models were fed with SHE and simultaneously subjected to treadmill exercise, which increased the expression of Myogenin, a key myogenic regulatory factor. In addition, there was an increase in the expression of AMPK- and Cytochrome C, both of which are important in mitochondrial biogenesis. Thus, the SHE from Hippocampus abdominalis can be a promising candidate as protein supplement aiding muscle development.

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