한국식품저장유통학회지 (Food Science and Preservation)

  • 제24권7호
  • /
  • Pages.891-897
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  • 2017
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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Md-ACS1 및 Md-ACO1 분자표지를 이용한 국내육성사과의 저장성 예측

Estimation of storability for Korean apples (Malus domestica) using Md-ACS1 and Md-ACO1 DNA marker

  • 권영순 (농촌진흥청 국립원예특작과학원 사과연구소) ;
  • 권순일 (농촌진흥청 국립원예특작과학원 사과연구소) ;
  • 김선애 (농촌진흥청 국립원예특작과학원 사과연구소) ;
  • 권헌중 (농촌진흥청 국립원예특작과학원 사과연구소) ;
  • 유진기 (경북대학교 원예과학과) ;
  • 류슬기 (경북대학교 원예과학과) ;
  • 강인규 (경북대학교 원예과학과) ;
  • 김정희 (농촌진흥청 국립원예특작과학원 사과연구소)
  • Kwon, Young Soon (Apple Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwon, Soon-Il (Apple Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Seon Ae (Apple Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kweon, Hun-Joong (Apple Research Institute, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoo, Jingi (Department of Horticultural Science, Kyungpook National University) ;
  • Ryu, Seulgi (Department of Horticultural Science, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Kim, Jeong-Hee (Apple Research Institute, National Institute of Horticultural and Herbal Science, RDA)
  • 투고 : 2017.09.06
  • 심사 : 2017.11.08
  • 발행 : 2017.11.30
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초록

사과는 에틸렌에 의해 호흡량이 일시적으로 상승하는 호흡급등형 과실이다. 에틸렌 발생은 세포벽분해효소 활성화와 세포벽 연화를 진행시켜 사과의 상품성과 저장성을 떨어뜨리는 원인이 된다. 사과의 에틸렌 생합성 과정에는 Md-ACS1 및 Md-ACO1 유전자가 연관되어 있으며, 두 유전자는 과실의 에틸렌 발생량과 경도에 영향을 미치는 것으로 알려져 있다. 본 연구는 사과 국내육성 28품종의 Md-ACS1 및 Md-ACO1 대립유전자형을 분석하고, 'Fuji(FJ)', 'RubyS(RS)', 'Hongro(HR)', 'Arisoo(AS)', 'Summer King(SK)', 'Greenball(GB)', 'Golden Supreme(GS)'을 대상으로 수확 후 25일까지의 에틸렌 발생량 및 상온저장($20^{\circ}C$) 20일 동안의 경도 연화율을 조사하였다. 그 결과, 낮은 에틸렌 발생량과 관련 된 대립유전자(favorable alleles0(FA)) ACS1-2, ACO1-1이 많을수록 에틸렌 발생량과 경도 연화율이 낮은 경향을 보였다. GS은 ACS1-1/1, ACO1-1/2(FA 1)으로 모든 품종 중 가장 높은 에틸렌 발생 수치와 경도 연화율를 보였다. SK와 GB은 ACS1-1/2, ACO1-1/2(FA 2)으로 ACS1-2/2, ACO1-1/2(FA 3)인 HR와 AS 보다는 높고 GS 보다 낮은 에틸렌 발생량과 경도 연화율을 보였다. ACS1-2/2, ACO1-1/1(FA 4)인 FJ는 RS를 제외한 모든 품종 중에 에틸렌 발생량과 경도 연화율이 가장 낮았다. 본 실험의 결과 Md-ACS1 및 Md-ACO1 대립유전자형과 사과의 에틸렌 발생량 및 경도 연화율은 상관성이 있는 것으로 확인되었다. 따라서, Md-ACS1 및 Md-ACO1 분자표지를 사과 국내육성품종의 저장성 예측과 육종 효율 향상을 위한 Marker-assisted selection에 활용할 수 있을 것으로 생각 된다.

Apple (Malus domestica) is a climacteric fruit because of its high respiration and ethylene production. Ethylene affects the fruit by decreasing its quality and storability. Md-ACS1 and Md-ACO1 genes are involved in ethylene biosynthesis in apple; the Md-ACS1-2 and Md-ACO1-1 alleles are associated with low ethylene production. We conducted an analysis to study Md-ACS1 and Md-ACO1, and to examine ethylene production and softening rate of fruit at room temperature ($20^{\circ}C$) storage in 'Fuji (FJ)', 'Golden Supreme (GS)', and 5 cultivars of Korean apples ('RubyS (RS)', 'Hongro (HR)', 'Arisoo (AS)', 'Summer King (SK)', 'Greenball (GB)'). The result showed that an increase in the number of the alleles (ACS1-2, ACO1-1) decreased the ethylene production and softening rate. The presence of ACS1-1/1, ACO1-1/2 was confirmed in GS and the highest ethylene production and softening rate was observed. Ethylene production and softening rate of SK and GB expressing ACS1-1/2, ACO1-1/2 were higher than that of HR and AS, expressing ACS1-2/2, ACO1-1/2, but lower than GS. FJ with ACS1-2/2, ACO1-1/1 showed the lowest ethylene production and softening rate among all cultivars except RS. The Md-ACS1 and Md-ACO1 DNA markers could potentially be used to estimate storability and applied in marker assisted selection the improve the efficiency of apple breeding.

키워드

참고문헌

  1. Ahn YJ, Choi JS, Min BH, Yi KU (1996) Effect of ethylene adsorbent on apple fruit quality during storage. J Natural sci Paichai Univ, 8, 169-173
  2. Taiz L, Zeiger E (2013) Plant Physiology. Life Science publishing co, Soeul, Korea, p 622-625
  3. Li T, Tan D, Yang X, Wang A (2013) Exploring the apple genome reveals six ACC synthase genes expressed during fruit ripening. Sci Hortic, 157, 119-123 https://doi.org/10.1016/j.scienta.2013.04.016
  4. Wakasa Y, Kudo H, Ishikawa R, Akada S, Senda M, Niizeki M, Harada T (2006) Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential. Postharvest Biol Technol, 39, 193-198 https://doi.org/10.1016/j.postharvbio.2005.10.005
  5. Costa F, Stella S, Van de Weg WE, Guerra W, Cecchinel M, Dallivina J, Koller B, Sansavini S (2005) Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh). Euphytica, 141, 181-190 https://doi.org/10.1007/s10681-005-6805-4
  6. Yang X, Song J, Campbell-Palmer L, Fillmore S, Zhang Z (2013) Effect of ethylene and 1-MCP on expression of genes involved in ethylene biosynthesis and perception during ripening of apple fruit. Postharvest Biol Technol, 78, 55-66 https://doi.org/10.1016/j.postharvbio.2012.11.012
  7. Johnston JW, Hewett EW, Hertog MLATM, Harker FR (2001) Temperature induces differential softening responses in apple cultivars. Postharvest Biol Technol, 23, 185-196 https://doi.org/10.1016/S0925-5214(01)00127-2
  8. Park JY, Kim KO, Yoo JG, Win NM, Lee JW, Choung MG, Jung HY, Kan IK (2016) Effects of aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) treatments on fruit quality attributes in cold-stored 'Jonathan' apples. Korean J Food Preserv, 23, 453-458
  9. Yoo JG, Kang BK, Lee JW, Kim DH, Lee DH, Jung HY, Choi DG, Choung MG, Choi IM, Kang IK (2015) Effect of preharvest and postharvest 1-methylcyclopropene (1-MCP) treatments on fruit quality attributes in cold-stored 'Fuji' apples. Kor J Hort Sci Technol, 33, 542-549
  10. Yoo JG, Kim DH, Lee JW, Choi DG, Han JS, Kwon SI, Kweon HJ, Kang IK (2013) Effect of preharvest sprayable 1-methylcyclopropene (1-MCP) treatment on fruit quality attributes in cold stored 'Gamhong' apples. Protected Hortic Plant Factory, 22, 279-283 https://doi.org/10.12791/KSBEC.2013.22.3.279
  11. Choi SJ (2005) Comparison of the change in quality and ethylene production between apple and peach fruits treated with 1-methylcyclopropene (1-MCP). Kor J Food Preserv, 12, 511-515
  12. Watkins CB (2008) Overview of 1-methylcyclopropene trials and uses for edible horticultural crops. HortScience, 43, 86-94
  13. Johnston JW, Hewett EW, Hertog MLATM (2002) Postharvest softening of apple (Malus domestica) fruit: A Review. N Z J Crop Hortic Sci, 30, 145-160 https://doi.org/10.1080/01140671.2002.9514210
  14. Nybom H, Ahmadi-Afzadi M, Sehic J, Hertog M (2013) DNA marker-assisted evaluation of fruit firmness at harvest and post-harvest fruit softening in a diverse apple germplasm. Tree Genet Genomes, 9, 279-290 https://doi.org/10.1007/s11295-012-0554-z
  15. Zhu Y, Mattheis J, Barritt B, Peace C (2009) Funtional genomics and marker development for apple sensory qualities. Final Report of USDA, ARS
  16. Sunako T, Sakuraba W, Senda M, Akada S, Ishikawa R, Niizeki M, Harada T (1999) An allele of the ripening-specific 1-aminocyclopropane-1-carboxylic acid synthase gene (ACS1) in apple fruit with a long storage life. Plant Physiol, 119, 1297-1303 https://doi.org/10.1104/pp.119.4.1297
  17. Zhu Y, Barritt BH (2008) Md-ACS1 and Md-ACO1 genotyping of apple (Malus$\times$domestica Borkh.) breeding parents and suitability for marker-assisted selection. Tree Genet Genomes, 4, 555-562 https://doi.org/10.1007/s11295-007-0131-z
  18. Kondo S, Uthaibutra J, Gemma H (1991) Comparison of 1-aminocyclopropane-1-carboxylic acid, abscisic acid and anthocyanin content of some apple cultivars during fruit growth and maturation. J Jpn Soc Hortic Sci, 60, 505-511 https://doi.org/10.2503/jjshs.60.505
  19. Costa F, Cappellin L, Farneti B, Tadiello A, Romano A, Soukoulis C, Sansavini S, Velasco R, Biasioli F (2014) Advances in QTL mapping for ethylene production in apple (Malus$\times$domestica Borkh.). Postharvest Biol Technol, 87, 126-132 https://doi.org/10.1016/j.postharvbio.2013.08.013
  20. Harada T, Sunako T, Wakasa Y, Soejima J, Satoh T, Niizeki M (2000) An allele of the 1-aminocyclopropane-1-carboxylate synthase gene (Md-ACS1) accounts for the low level of ethylene production in climacteric fruits of some apple cultivars. Theor Appl Genet, 101, 742-746 https://doi.org/10.1007/s001220051539
  21. Oraguzie NC, Iwanami H, Soejima J, Harada T, Hall A (2004) Inheritance of the Md-ACS1 gene and its relationship to fruit softening in apple (Malus domestica Borkh.). Theor Appl Genet, 108, 1526-1533 https://doi.org/10.1007/s00122-003-1574-8
  22. Nybom H, Sehic J, Garkava-Gustavsson L (2008) Modern apple breeding is associated with a significant change in allelic ratio of the ethylene production gene Md-ACS1. J Hortic Sci Biotechnol, 83, 673-677 https://doi.org/10.1080/14620316.2008.11512442

피인용 문헌

  1. Validation Assay of Md-ACS1, Md-ACO1, and Md-PG1 Molecular Markers Associated with Storability in Apples vol.52, pp.4, 2017, https://doi.org/10.9787/kjbs.2020.52.4.322
  2. Effect of application time of 1-methylcyclopropene treatment on fruit quality attributes in ‘Fuji’ apples during simulated marketing period vol.28, pp.3, 2017, https://doi.org/10.11002/kjfp.2021.28.3.318