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황련과 Pelargonium sidoides 복합제제의 호흡기 감염 세균에 대한 항균 효과

Anti-bacterial effect of fusion formulation of Coptis rhizoma and Pelargonium sidoides on the growth of bronchial diseases bacteria

  • 투고 : 2017.10.25
  • 심사 : 2017.11.07
  • 발행 : 2017.11.30

초록

Objective : Coptis rhizoma is traditional herb in Korean medicine, and Pelargonium sidoides extract has been used for relief of acute bronchitis in Western medicine. The present study examined the antibacterial effect of fusion formulation of Coptis rhizoma extract and Pelargonium sidoides extract against bronchial diseases bacteria. Methods : Test sample, fusion formulation of Korean and Western medicine, was prepared by mixing Coptis rhizoma extract and Pelargonium sidoides extract at a ratio of 1:2 (w/w). Antimicrobial properties of test sample were determined by agar diffusion assay and minimum inhibitory concentration (MIC) against bronchus diseases bacteria. Results : In agar diffusion assay, the highest amount of test sample (4 mg/disk) exhibited antibacterial activity against all microorganisms tested. Test sample showed the high activity for S. aureus (19.5 mm), C. diptheriae (16.5 mm), A. fumigatus (19.3 mm), F. nucleatum (22.7 mm) and Mycobactrium sp. (17.3 mm), whereas it showed a low activity for K. pneumonia (9.7 mm). The MIC value was determined as $250{\mu}g/m{\ell}$ against C. diptheriae. Test sample showed better growth inhibitory effects against S. aureus and A. fumigatus with the MIC valus of $125{\mu}g/m{\ell}$. Conclusion : These results suggest the possibility of application to chronic respiratory diseases of fusion formulation of Korean and Western medicine, which was prepared with Coptis rhizoma extract and Pelargonium sidoides extract.

키워드

참고문헌

  1. Kim SW. Bacterial upper respiratory infections. Infect Chemother. 2011;43(5):383-389. https://doi.org/10.3947/ic.2011.43.5.383
  2. Vestbo J, Prescott E, Lange P. Association of chronic mucus hypersecretion with FEV1 decline and chronic obstructive pulmonary disease morbidity. Copenhagen City Heart Study Group. Am J Respir Crit Care Med. 1996;153(5): 1530-1535. https://doi.org/10.1164/ajrccm.153.5.8630597
  3. Jung SW, Kim SH. Analysis of antimicrobial resistance of bacterial respiratory pathogens isolated from patients shown respiratory infections in south Korea. Public Health Weekly Report (KCDC). 2014;7(24):513-518.
  4. Park HS, Lee BW, Lee BK. A study on the comparative method of prescription using gunsinjwasa theory. Herb Formula Sci. 2014;22(2): 45-54. https://doi.org/10.14374/HFS.2014.22.2.045
  5. Kim SJ, Jang SK, Jung HJ, Jung SK, Lee BJ. A study on the fever of unknown origin indicated by Janggam disease. J Physiol & Pathol Korean Med. 2014;28(6):683-688. https://doi.org/10.17208/kjopp.2014.12.28.6.683
  6. Korea food and drug administration. The Korean pharmacopoeia tenth edition. Seoul : Korea food and drug administration. 2007: 995-996.
  7. Sikder MA, Lee HJ, Lee SY, Bae HS, Kim JH, Chang GT, Lee CJ. Effect of berberine on MUC5AC mucin gene expression and mucin production from human airway epithelial cells. Biomol Ther. 2011;19(3):320-323. https://doi.org/10.4062/biomolther.2011.19.3.320
  8. Zhou L, Wang X, Yang Y, Wu L, Li F, Zhang R, Yuan G, Wang N, Chen M, Ning G. Berberine attenuates cAMP-induced lipolysis via reducing the inhibition of phosphodiesterase in 3T3-L1 adipocytes. Biochim Biophys Acta. 2011;1812(4): 527-535. https://doi.org/10.1016/j.bbadis.2010.10.001
  9. Brendler T, van Wyk BE. A historical, scientific and commercial perspective on the medicinal use of Pelargonium sidoides (Geraniaceae). J Ethnopharmacol. 2008;119:420-433. https://doi.org/10.1016/j.jep.2008.07.037
  10. Kim SK, Chang J. Respiratory symptoms relievers - Antitussives, mucolytics, antihistamines. Tuberc Respir Dis. 2006;60(3):261-269. https://doi.org/10.4046/trd.2006.60.3.261
  11. Rosenfeld RM, Andes D, Bhattacharyya N, Cheung D, Eisenberg S, Ganiats TG, et al. Clinical practice guideline: adult sinusitis. Otolaryngol Head Neck Surg 2007;137(3 Suppl): S1-31. https://doi.org/10.1016/j.otohns.2007.06.726
  12. Lee IS, Choi MC, Moon HY. Effect of Platycodon grandiflorum A. DC extract on the bronchus diseases bacteria. Korean J Biotechnol Bioeng, 2000;15(2):162-166.
  13. Song JH. Current status and future strategies of antimicrobial resistance in Korea. Korean J Med. 2009;77(2):143-151.
  14. Lee EK. Analysis of the changes in antibiotic use and resistance. Health and Welfare Policy Forum. 2003;77:72-82.
  15. The Korean association of university professors of herbology. Herbology. Seoul : Younglimsa. 2008: 218-220.
  16. Yoon KR, Kim YJ, Lee E, Lee JM. Antiinflammatory effect of Coptidis rhizoma. Kor J Herbology. 2009;24(3):79-86.
  17. Shin JI, Seo YK. Antibacterial effects of Corptis japonica against Helicobacter pylori. Korean J Orient Int Med. 2003;24(2):269-282.
  18. Kim YJ, Lee MJ, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant activity of water-extract from Coptis chinensis Franch. Korean J Life Sci. 2000;10(3):241-246.
  19. Kim HK, Hong SU. The anti-inflammatory effects of Huang-Lyun (Coptidis rhizoma, CR) on injured tissue after burn elicitation. J Korean Oriental Med. 2011;32(2):1-13.
  20. Seo HS. The Experimental Study on Antibacterial Potency of Coptidis rhizoma extract compare with quantity on Staphylococcus aureus. J Pharmacopunct. 2007;10(3): 47-52. https://doi.org/10.3831/KPI.2007.10.3.047
  21. Kim KB, Lee HT, Ku KH, Hong JW, Cho SI. Review of pharmacological effects of Coptidis rhizoma and its bioactive compounds. J Korean Oriental Med. 2012;33(3):160-183.
  22. Yan D, Jin C, Xiao X, Dong X. Antimicrobial properties of berberines alkaloids in Coptis chinensis Franch by microcalorimetry. J Biochem Biophys Methods. 2008;70(6):845-849. https://doi.org/10.1016/j.jbbm.2007.07.009
  23. Sin KH, An DK, Woo ER, Park HK, Lee JS, Yun WS. A study on extraction efficiency of berberine in Coptidis rhizoma. J Kor Analyical Sci. 1997; 10(2):83-91.