한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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고지방 식이로 유도된 실험동물의 당뇨성 인지기능 장애에 대한 고사리 아세트산에틸 분획물의 개선효과

Ethyl acetate fraction from Pteridium aquilinum ameliorates cognitive impairment in high-fat diet-induced diabetic mice

  • 권봉석 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 궈텐쟈오 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 강진용 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박상현 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 강정은 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 이창준 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 이욱 (국립산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원)
  • Kwon, Bong Seok (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Guo, Tian Jiao (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jeong Eun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Chang Jun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2017.06.09
  • 심사 : 2017.07.15
  • 발행 : 2017.12.31
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초록

본 연구는 고지방 식이를 통해 유도되는 혈당 상승과 이로 인한 인지기능 장애에 대한 고사리(Pteridium aquilinum) 아세트산에틸 분획물(EFPA)의 개선 효과를 확인하기 위해서 수행되었다. 고지방 식이를 섭취시킨 실험동물에 고사리 아세트산에틸 분획물을 식이를 하였을 때, 공복혈당을 낮추었고 인슐린 저항성을 확인하기 위한 내당능 시험에서 개선효과를 나타내었다. 또한 실험동물의 혈액을 이용한 혈청분석 결과 혈청 내 총 콜레스테롤(TCHO)과 저밀도지단백질 콜레스테롤(LDLC)과 같은 지방질 대사 수준이 유의적으로 개선되었다. 실험동물을 이용한 행동학적 시험(Y-maze, passive avoidance, Morris water maze tests)을 통해 인지기능 개선효과를 확인해본 결과, 공간인지 능력, 단기 학습 능력, 장기 기억 능력이 고지방 식이군(HFD)에 비해 유의적으로 개선되었다. 또한 동물 행동 실험 후 적출된 뇌 조직에서 산화방지 효소인 초과산화물제거효소(SOD)의 함량과 지방질과산화 정도(MDA level)를 측정해본 결과 고사리 아세트산에틸 분획물이 조직에서의 산화방지 능력을 증가시켜 주는 것을 확인 할 수 있었다. 그리고 인지능력에 영향을 주는 아세틸콜린(ACh)과 아세틸콜린 가수분해효소(AChE)에 미치는 영향을 확인해 본 결과, 아세틸콜린 가수분해효소의 활성이 억제되었고 그 결과 아세틸콜린의 함량이 증가하였다. 고지방 식이로 유도되는 혈당 상승 및 인지기능 장애의 개선에 도움이 되는 고사리 아세트산에틸 분획물의 주요 생리활성 물질이 무엇인지 확인하기 위하여 QTRAP LC-MS/MS를 시행한 결과, 주요 물질은 kaempferol-3-O-glucoside로 동정되었고 이 이외에 caffeolyshikimic acid, quercetin-3-O-glucoside, kaempferol-3-O-rutinoside 등이 확인 되었다. 이러한 연구결과를 고려할 때, 캠페롤(kaempferol) 등과 같은 플라보노이드류와 폴리페놀 화합물을 함유한 고사리 아세트산에틸 분획물(EFPA)은 고지방 식이로 인한 혈당 상승과 이로 인한 인지기능 장애의 개선에 도움을 줄 수 있는 건강 기능성 식품 소재로 이용될 수 있을 것으로 판단된다.

The potential of the ethyl acetate fraction from Pteridium aquilinum (EFPA) to improve the cognitive function in high-fat diet (HFD)-induced diabetic mice was investigated. EFPA-treatment resulted in a significant improvement in the spatial, learning, and memory abilities compared to the HFD group in behavioral tests, including the Y-maze, passive avoidance, and Morris water maze. The diabetic symptoms of the EFPA-treated groups, such as fasting glucose and glucose tolerance, were alleviated. The administration of EFPA reduced the acetylcholinesterase (AChE) activity and malondialdehyde (MDA) content in mice brains, but increased the acetylcholine (ACh) and superoxide dismutase (SOD) levels. Finally, kaempferol-3-o-glucoside, a major physiological component of EFPA, was identified by using high-performance liquid chromatography coupled with a hybrid triple quadrupole-linear ion trap mass spectrometer (QTRAP LC-MS/MS).

키워드

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