The Journal of the Korean dental association (대한치과의사협회지)

The Korean Dental Association (대한치과의사협회)
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Effect of Epigallocatechin-3-Gallate on the alveolar bone remodeling and arthritis in collagen-induced arthritis model in mice

콜라겐 유도 관절염 모델에서 동반된 치주염 유발시 EGCG가 치주염 치료에 미치는 효과에 관한 연구

  • Cho, In-Woo (Department of Periodontology,College of Dentistry, Dankook University) ;
  • Yim, Seong-Jun (Department of Periodontology,College of Dentistry, Dankook University) ;
  • Shin, Hyun-Seung (Department of Periodontology,College of Dentistry, Dankook University) ;
  • Park, Jung-Chul (Department of Periodontology,College of Dentistry, Dankook University)
  • 조인우 (단국대학교 치과대학 부속치과병원 치주과학교실) ;
  • 임성준 (단국대학교 치과대학 부속치과병원 치주과학교실) ;
  • 신현승 (단국대학교 치과대학 부속치과병원 치주과학교실) ;
  • 박정철 (단국대학교 치과대학 부속치과병원 치주과학교실)
  • Received : 2015.12.15
  • Accepted : 2016.02.17
  • Published : 2016.04.01
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Abstract

The aim of this study was to evaluate the effect of Epigallocatechin-3-Gallate (EGCG) on the alveolar bone metabolism in a collagen-induced arthritis (CIA) model in mice to enhance the understanding of rheumatoid arthritis (RA)-associated alveolar bone loss. Following the induction of CIA in animals (mice, n=16), mandibles were retrieved for micro-computed tomography (micro-CT) and isolation of alveolar bone cells (ABCs). In vitro osteogenic potentials of ABCs were evaluated and the mRNA expression of downstream effector genes was assessed. CIA was successfully induced in all animals, and micro-CT data showed that alveolar bone loss was significantly increased in the CIA group while the treatment of EGCG prevented the alveolar bone resorption. Osteogenesis by ABCs was significantly increased in the CIA+EGCG group in vitro. The analysis of mRNA expressions showed that osteoclastogenesis-associated genes were increased in CIA group while bone protecting genes were upregulated in EGCG treated group. The results demonstrate that EGCG downregulated the alveolar bone resorption in a CIA model in mice, and upregulation of bone protecting genes appear to be involved. Further studies are warranted.

Keywords

Acknowledgement

Supported by : 한국연구재단

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