Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling

  • Kim, Young Eun (Cosmecutical R&D Center, HP&C, Seowon University) ;
  • Choi, Hyung Chul (Cosmecutical R&D Center, HP&C, Seowon University) ;
  • Lee, In-Chul (Department of Cosmetic Science & Engineering, Seowon University) ;
  • Yuk, Dong Yeon (Cosmecutical R&D Center, HP&C, Seowon University) ;
  • Lee, Hyosung (Department of Pharmaceutical Science & Engineering, Seowon University) ;
  • Choi, Bu Young (Department of Pharmaceutical Science & Engineering, Seowon University)
  • Received : 2016.08.16
  • Accepted : 2016.09.22
  • Published : 2016.11.01
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Abstract

3-Deoxysappanchalcone (3-DSC) has been reported to possess anti-allergic, antiviral, anti-inflammatory and antioxidant activities. In the present study, we investigated the effects of 3-DSC on the proliferation of human hair follicle dermal papilla cells (HDPCs) and mouse hair growth in vivo. A real-time cell analyzer system, luciferase assay, Western blot and real-time polymerase chain reaction (PCR) were employed to measure the biochemical changes occurring in HDPCs in response to 3-DSC treatment. The effect of 3-DSC on hair growth in C57BL/6 mice was also examined. 3-DSC promoted the proliferation of HDPCs, similar to Tofacitinib, an inhibitor of janus-activated kinase (JAK). 3-DSC promoted phosphorylation of ${\beta}$-catenin and transcriptional activation of the T-cell factor. In addition, 3-DSC potentiated interleukin-6 (IL-6)-induced phosphorylation and subsequent transactivation of signal transducer and activator of transcription-3 (STAT3), thereby increasing the expression of cyclin-dependent kinase-4 (Cdk4), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF). On the contrary, 3-DSC attenuated STAT6 mRNA expression and IL4-induced STAT6 phosphorylation in HDPCs. Finally, we observed that topical application of 3-DSC promoted the anagen phase of hair growth in C57BL/6 mice. 3-DSC stimulates hair growth possibly by inducing proliferation of follicular dermal papilla cells via modulation of $WNT/{\beta}$-catenin and STAT signaling.

Keywords

References

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