Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Toxicity Assessment of Heavy Metals (As, Cr and Pb) Using the Rates of Survival and Population Growth in Marine Rotifer, Brachionus plicatilis

해산로티퍼 (Brachionus plicatilis)의 생존 및 개체군 성장률을 이용한 중금속 (As, Cr, Pb) 독성평가

  • Lee, Ju-Wook (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Ryu, Hyang-Mi (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Heo, Seung (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center) ;
  • Hwang, Un-Ki (National Fisheries Research & Development Institute, West Sea Fisheries Research Institute, Marine Ecological Risk Assessment Center)
  • 이주욱 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 류향미 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 허승 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 황운기 (국립수산과학원 서해수산연구소 해양생태위해평가센터)
  • Received : 2016.07.25
  • Accepted : 2016.09.12
  • Published : 2016.09.30
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Abstract

Toxicity assessment of heavy metals (As, Cr and Pb) has been investigated by using the rate of survival and population growth(r) of marine rotifer, Brachionus plicatilis. The survival rate was determined after 24 hours of exposure to As, Cr and Pb. As and Cr reduced survival rate in dose-dependent manner and a significant reduction were occurred at concentration of greater than 30 and $150mg\;L^{-1}$, but Pb had no effect on survival rate. The r was determined after 72 hours of exposure to As, Cr and Pb. As, Cr and Pb reduced r in dose-dependent manner and a significant reduction were occurred at concentration of greater than 5, 25 and $50mg\;L^{-1}$. The toxicity of heavy metals were ranked As>Cr>Pb, with $EC_{50}$ values of 12.98, 82.34 and $110.14mg\;L^{-1}$, respectively. The no-observed-effect-concentration (NOEC) of r in As, Cr and Pb exposure were 1, 12.5 and $50mg\;L^{-1}$, respectively. The lowest-observed-effect-concentration (LOEC) of r in As, Cr and Pb exposure were 5, 25, and $50mg\;L^{-1}$, respectively. From the results, the concentration of As, Cr and Pb (greater than 5, 25 and $50mg\;L^{-1}$, respectively) have toxic effect on the r of B. plicatilis in natural ecosystems. These results (including NOEC and $EC_{50}$) might be useful for the mixing toxicity assessment and toxic guide line of heavy metals in marine ecosystems.

해산로티퍼 (Brachionus plicatilis)의 생존율 및 개체군 성장률을 사용하여 중금속 As, Cr 및 Pb에 대한 독성평가를 수행하였다. 중금속에 24시간 노출한 생존율은 As와 Cr의 30과 $150mg\;L^{-1}$ 농도에서 급격한 감소가 시작되어 농도의존적으로 감소하였으나, Pb에서는 영향이 나타나지 않았다. 중금 속에 72시간 노출한 개체군 성장률은 As, Cr 및 Pb의 5, 25 및 $50mg\;L^{-1}$ 농도에서 급격한 감소가 시작되어, 3종 중금속에서 모두 농도의존적으로 감소하는 경향을 보였다. As, Cr 및 Pb에 노출된 개체군 성장률의 $EC_{50}$ 값은 각각 12.98, 82.34 및 $110.14mg\;L^{-1}$이고 독성의 세기는 As>Cr>Pb로 생존율과 동일하였다. 또한 As, Cr 및 Pb에 대한 개체군 성장률의 NOEC는 각각 1, 12.5 및 $50mg\;L^{-1}$, LOEC는 각각 5, 25 및 $50mg\;L^{-1}$로 나타났다. 해양환경에서 각각의 LOEC 이상의 농도는 B. plicatilis에게 독성영향을 미칠 수 있는 농도로 판단되며, NOEC와 $EC_{50}$ 값은 WET test를 위한 혼합독성과 해양생태계 내 중금속독성에 대한 가이드라인으로 적절하게 활용될 수 있을 것이다.

Keywords

References

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