Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Study on Antioxidant Activity and Cytotoxicity in Cancer Cells of Extract from Waxy Sorghum fermented with Phellinus linteus Mycelium

상황버섯 균사체를 이용한 찰수수 발효 추출물의 항산화 활성 및 암세포에 대한 세포 독성 연구

  • Zhang, Mei-Wei (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Park, Mi-Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Meera (Dept. of Food Science and Nutrition, Kyungpook National University)
  • 장매위 (경북대학교 식품영양학과) ;
  • 박미혜 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과)
  • Received : 2016.08.04
  • Accepted : 2016.09.20
  • Published : 2016.10.31
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Abstract

Studies have been conducted on fermentation products known to increase biological activity through bioconversion of mycelium. In this study, ethanol extract of waxy sorghum (WS) and ethanol extract of waxy sorghum fermented with Phellinus linteus mycelium (WSPM) were prepared, and functional component contents, antioxidant activity, and cytotoxicity were analyzed. Total polyphenol contents and total flavonoid contents of WSPM were higher than those of WS. In addition, the ${\beta}-glucan$ content of WS was higher than that of WSPM. DPPH and ABTS radical scavenging activities showed that WSPM had higher antioxidant activity than WS at all concentrations. Analysis of SOD-like activity also showed higher antioxidant activity in WSPM. MTT assay demonstrated that WSPM exhibited high inhibitory activity in all cancer cells, and in particular, in HeLa cells with the highest inhibition. A concentration-dependent increase in anticancer activities of WS and WSPM was detected in all cancer cells, which was identical to the SRB assay result. MTT and SRB assay showed the increased cytotoxicity of WSPM in cancer cells. Therefore, it is expected that WSPM can be used as a functional food material.

Keywords

References

  1. Awika JM, Yang L, Browning JD, Faraj A (2009) Comparative antioxidant, antiproliferative and phase II enzyme inducing potential of sorghum (Sorghum bicolor) varieties. LWT-Food Sci Technol 42(6): 1041-1046. https://doi.org/10.1016/j.lwt.2009.02.003
  2. Bae MJ, Kim KJ, Kim SJ, Ye EJ (2007) Effect of mycelia extracts from Lentinus edoeds mushroom cultured Astragalus membranceus Bunge on anticancer and antiallergy activities. J Korean Soc Food Sci Nutr 36(1): 8-13. https://doi.org/10.3746/jkfn.2007.36.1.008
  3. Blois MS (1958) Antioxidant determination by use of a stable free radical. Nature 181(4617): 1199-1200. https://doi.org/10.1038/1811199a0
  4. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47(4): 936-942.
  5. Chung KS, Kim SS, Kim HS, Kim KY, Han MW (1993) Effect of Kp, an antitumor protein polysaccharide from mycelial culture of Phellinus linteus on the humoral immune response of tumor bearing ICR mice to sheep red blood cells. Arch Pharm Res 16(4): 336-338. https://doi.org/10.1007/BF02977527
  6. Doll R, Peto R (1981) The causes of cancer: Quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66(6): 1191-1308.
  7. Dykes L, Rooney LW (2006) Sorghum and millet phenols and antioxidants. J Cereal Sci 44(3): 236-251. https://doi.org/10.1016/j.jcs.2006.06.007
  8. Han MD, Kim YH, Kim WJ (2014) In vivo growth inhibition of Sarcoma-180 cells by a ${\beta}$-glucan from the mushroom Ganoderma lucidum. J Life Sci 24(7): 721-727. https://doi.org/10.5352/JLS.2014.24.7.721
  9. Hong JY (2014) Lipid lowering effect of solid fermented Panax ginseng by Phellinus linteus mycelium. MS Thesis Pusan National University, Pusan. pp. 38, 55, 57.
  10. Hulse JH, Laing EM, Pearson OE (1980) Sorghum and the Millets: Their Composition and Nutritive Value. Pittsburgh, USA.
  11. Hwang YJ, Noh GW, Kim SH (2003) Effect of Inonotus obliquus extracts on proliferation and caspase-3 activity in human gastrointestinal cancer cell lines. J Korean Nutr Soc 36(1): 18-30.
  12. Jeon YH, Choi SW, Kim MR (2009) Antimutagenic and cytotoxic activity of ethanol and water extracts from Rubus coreanum. Korean J Food Cookery Sci 25(3): 379-386.
  13. Jin SY (2007) Antioxidant activity in pomegranate and development of the maejakgwa added pomegranate extract. Ph D Dissertation Sookmyung Women's University, Seoul. pp. 68-69.
  14. Jeung EM, Kim HY, Hwang IG, Jeong JH, Yu KW, Lee JS, Jeong HS (2010) Changes of antioxidant activities on cultured ginseng with mushroom mycelia during cultivation. J Korean Soc Food Sci Nutr 39(9): 1346-1352. https://doi.org/10.3746/jkfn.2010.39.9.1346
  15. Kim H, Yoon HS, Jeong JH, Jeong HS, Hwang J, Yu KW (2010) Enhancement of immunostimulation by fractionation of active polysaccharide from fermented ginseng with Phellinus linteus mycelium in solid culture. Korean J Food Sci Technol 42(2): 223-232.
  16. Kim JS, Jung HJ, Yang JF, Eun YC, Sa YJ, Yu CY, Heo SI, Pack DS, Chung IM, Kim MJ (2011) Anticancer Activity of Apigenin isolated from Sorghum (Sorghum bicolor L. Moench). Abstract No II-60 presented Korean Society of Medicinal Crop Science, Eumseong, CB, Korean.
  17. Kim KO (2004) Effect of Sorghum bicolor L. Moench (Sorghum, su-su) extracts on mouse immune cell activation. MS Thesis Sookmyung Women's University, Seoul. pp. 78-80.
  18. Kim S (2012) The physiochemical characteristics and whitening and antioxidant effects of colored rice cultured with the mycelium of Phellinus baumii Pilst. MS Thesis Chonnam National University, Gwangju. p. 30.
  19. Kim SH (2002) Pharmacological activities of Phellinus linteus. MS Thesis Sookmyung Women's University, Seoul. pp. 58, 60.
  20. Lee BE, Ryu SY, Kim EH, Kim YH, Kwak KA, Song HY (2012）Immunostimulating effect of mycelium extract of Phellinus linteus. Kor J Pharmacog 43(2): 157-162.
  21. Lee HD, Lee KS (2009) ${\beta}$-Glucan and glucosamine contents in various cereals cultured with mushroom mycelia. Kor J Mycol 37(2): 167-172. https://doi.org/10.4489/KJM.2009.37.2.167
  22. Lee JJ (2009) Persimmon leaf pan fired tea and Ganoderma lucidum mushroom mycelia fermented persimmon leaf tea's elemental analysis and antioxidization effect. MS Thesis Daegu Haany University, Daegu. pp. 30-31.
  23. Lee JY, Kim BK, Park HJ (2013) Antioxidant activities and quality characteristics of fermented Codonopsis lanceolata tea according to heating processes. Korean J Food Nutr 26(4): 693-699. https://doi.org/10.9799/ksfan.2013.26.4.693
  24. Marklund S, Marklund G (1974) Involvement of super oxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47(3): 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  25. Moreno MI, Isla MI, Sampietro AR, Vattuone MA (2000) Comparison of the free radical scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71(1-2): 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  26. Park MJ (2011) The effects of hangover cure and improve of liver function activity of mushroom mycelia cultured with medicinal plant as substrate. MS Thesis Andong National University, Andong. pp. 70-71.
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice Evans C (1998) Screening of dietary carotenoids and carotenoid rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylene benzothiazoline-6-sulfonic acid) radical cation decolorization assay. Meth Enzymol 299(1): 379-389.
  28. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26(9-10): 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  29. Ryu JS (2011) Chemical composition and biological functions of red ginseng (Panax ginseng C.A. Meyer) fermented by Phelinus linteus mycelia. Ph D Dissertation Dankook University, Yongin. p. 65.
  30. Shin YK, Jang HS, Kim JS, Ryu HY, Kim JK, Kwun IS, Sohn HY (2008) Solid fermentation of medicinal herb using Phellinus baumii mycelium and antithrombin and antioxidation activity of its methanol extract. Kor J Microbiol Biotechnol 36(3): 201-208.
  31. Sin OS (2004) In vitro effect of extracts of Phellinus baumii and Ganoderma lucidum on antiviral and immuno activity. MS Thesis Korea University, Seoul. pp. 17-18.
  32. Shon MY (2007) Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Indus Nutr 12(2): 51-57.

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