한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제24권5호
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  • Pages.408-419
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  • 2016
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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황기 지상부 다당체의 면역 및 백신보조 효과

Adjuvant Effect of Polysaccharides from Aboveground Parts of Astragalus membranaceus

  • 양수진 (강원대학교 생약자원개발학과) ;
  • 이시영 (강원대학교 생약자원개발학과) ;
  • 이한나 (강원대학교 생약자원개발학과) ;
  • 박영철 (대구가톨릭대학교 GLP 센터) ;
  • 최선강 (강원대학교 농생명산업학과) ;
  • 유창연 (강원대학교 식물자원응용공학과) ;
  • 정일민 (건국대학교 응용생물과학과) ;
  • 임정대 (강원대학교 생약자원개발학과)
  • Yang, Su Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Lee, Si Young (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Lee, Hannah (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Park, Yeong Chul (GLP Center, Catholic University of Daegu) ;
  • Choi, Seon Kang (Department of Agricultural Life Science, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Applied Plant Sciences, Kangwon National University) ;
  • Chung, Ill Min (Department of Applied Bioscience, Konkuk University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
  • 투고 : 2016.09.05
  • 심사 : 2016.10.17
  • 발행 : 2016.10.30
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초록

Background: In recent years, adjuvants have received increasing attention owing to the development of purified subunit and synthetic vaccines which are poor immunogens and require additional adjuvants to evoke an immune response. Therefore, immunologic adjuvants have been developed and tested. Plant polysaccharides have been recognized as effective biological response modifiers with low toxicity. Methods and Results: In this study, the polysaccharide from the aboveground part of Astragalus membranaceus Bunge containing immunomodulating arabino-3,6-galactan was evaluated for its hemolytic activity and adjuvant potential in the specific cellular and humoral immune responses to ovalbumin. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge was co-immunized with the purified Vi capsular polysaccharide of Salmonella typhi vaccine in mice. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge did not induce any hemolytic activity or side effects at doses up to $500{\mu}g/m{\ell}$. The concanavalin A-, lipopolysaccharide-, and ovalbumin-induced splenocyte proliferation and serum ovalbumin-specific IgG, IgG1 and IgG2b antibody titers in immunized mice were significantly enhanced by AMA. Pharmacological data revealed that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge increased antigen-specific antibody levels in immunized mice. The polysaccharide from the aboveground part of Astragalus membranaceus Bunge-adjuvanted purified Vi capsular polysaccharide of Salmonella typhi vaccine improved the proliferation of splenocytes and macrophages as well as stimulated cytokine production. Conclusions: These results suggest that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge-adjuvanted vaccines enhanced humoral and cellular immunity and that the polysaccharide from the aboveground part of Astragalus membranaceus Bunge is a safe and efficacious adjuvant candidate suitable for use in prophylactic and therapeutic vaccines.

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