Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Tissue Type Expression of Phospholipase C β3 in Olive Flounder (Paralichthys olivaceus) Following Various Stimulation

다양한 자극에 의한 넙치의 Phospholipase C β3 조직별 발현 분석

  • Received : 2016.06.28
  • Accepted : 2016.08.24
  • Published : 2016.10.31
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Abstract

Phospholipase C is a key enzyme of signaling pathways hydrolyzed phosphatidylinositol 4,5-bisphosphate to generate 2 second messengers. Among the PLC, $PLC-{\beta}$ subfamily consisted of 4 isoforms, $PLC-{\beta}$ 1~4. Here, we studied the tissue specific expression of $PLC-{\beta}3$ in olive flounder (Paralichthys olivaceus) following external stimulation like lipopolysaccharide (LPS), concanavalin A (ConA) and environmental stress compared with the inflammatory cytokines IL-1b. $PoPLC-{\beta}3$ gene transcripts has the effect in stimulated tissue compared to control. These results provide what we sure to be a important role for $PLC-{\beta}3$ activity in tissue and verify $PLC-{\beta}3$ as potential immune enzyme for signal transduction.

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References

  1. An, K. W..Kim, N. N..Shin, H. S..Kil, G. S. & Choi, C. Y.(2010). Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 156(2), 262-268. https://doi.org/10.1016/j.cbpa.2010.02.013
  2. Bach, T. L..Chen, Q. M..Kerr, W. T..Wang, Y..Lian, L..Choi, J. K..Wu, D..Kazanietz, M. G..Koretzky, G. A..Zigmond, S. & Abrams, C. S.(2007). Phospholipase c $\beta$ is critical for T cell chemotaxis. The Journal of Immunology, 179(4), 2223-2227. https://doi.org/10.4049/jimmunol.179.4.2223
  3. Barton, B. A. & Iwama, G. K.(1991). Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual Review of Fish Diseases, 1, 3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  4. Beitinger, T. L. & Fitzpatrick, L. C.(1979). Physiological and ecological correlates of preferred temperature in fish. American Zoologist, 19(1), 319-329. https://doi.org/10.1093/icb/19.1.319
  5. Berridge, M. J. & Irvine, R. F.(1989). Inositol phosphates and cell signalling. Nature, 341, 197-205. https://doi.org/10.1038/341197a0
  6. Conte, F. S.(2004). Stress and the welfare of cultured fish. Applied Animal Behaviour Science, 86(3), 205-223. https://doi.org/10.1016/j.applanim.2004.02.003
  7. Giulietti, A..Oververgh, L..Valckx, D..Decallonne, B..Bouillon, R. & Mathieu, C. (2001). An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods, 25(4), 386-401. https://doi.org/10.1006/meth.2001.1261
  8. Han, S. K..Mancino, V. & Simon, M. I. (2006). Phospholipase C ${\beta}3$ mediates the scratching response activated by the histamine H1 receptor on C-fiber nociceptive neurons, Neuron, 52(4), 691-703. https://doi.org/10.1016/j.neuron.2006.09.036
  9. Hansmann, R. H..Mauser, E..Sandler, T..Uhr, E..Wood, I. & Young, D.(1989). Studies of Inositol Phospholipid Specific Phospholipase C. Science, 244, 546. https://doi.org/10.1126/science.2541501
  10. Kremer, J. M..Lawrence, D. A..Jubiz, W..Digiacomo, R..Rynes, R..Bartholomew, L. E. & Sherman, M.(1990). Dietary fish oil and olive oil supplementation in patients with Rheumatoid Arthritis clinical and immunologic effects. Arthritis & Rheumatism, 33(6), 810-820. https://doi.org/10.1002/art.1780330607
  11. Noh, D. Y..Shin, S. H. & Rhee, S. G. (1995). Phosphoinositide-specific phospholipase C and mitogenic signaling. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1242(2), 99-113. https://doi.org/10.1016/0304-419X(95)00006-0
  12. Rhee, S. G..Choi, K. D..Pan, G..Sadowski, P. D..Wynn, R. M..Davie, J. R. & Brownlee, M.(1992). Regulation of inositol phospholipidspecific phospholipase C isozymes. Regulation, 267(18), 12393.
  13. Rossler, P..Kroner, C..Freitag, J. Noe, J. & Breer, H.(1998). Identification of a phospholipase C $\beta$ subtype in rat taste cells. European journal of cell biology, 77(3), 253-261. https://doi.org/10.1016/S0171-9335(98)80114-3
  14. Singh, S. M. & Murray, D.(2003). Molecular modeling of the membrane targeting of phospholipase C pleckstrin homology domains. Protein Science, 12(9), 1934-1953. https://doi.org/10.1110/ps.0358803
  15. Sternweis, P. C. & Smrcka, A. V.(1992). Regulation of phospholipase C by G proteins. Trends in Biochemical Sciences, 17(12), 502-506. https://doi.org/10.1016/0968-0004(92)90340-F
  16. Swain, P..Nayak, S. K..Nanda, P. K. & Dash, S.(2008). Biological effects of bacterial lipopolysaccharide (endotoxin) in fish: a review. Fish & shellfish immunology, 25(3), 191-201. https://doi.org/10.1016/j.fsi.2008.04.009
  17. Testerink, C. & Munnik, T.(2005). Phosphatidic acid: a multifunctional stress signaling lipid in plants. Trends in plant science, 10(8), 368-375. https://doi.org/10.1016/j.tplants.2005.06.002
  18. Vatter, P..Schuhholz, J..Koenig, C..Pfreimer, M. & Moepps, B.(2016). Ligand-dependent serum response factor activation by the human CC chemokine receptors CCR2a and CCR2b is mediated by G proteins of the Gq family. Journal of leukocyte biology, 99(6), 979-991. https://doi.org/10.1189/jlb.2MA0815-386R
  19. Wang, Z..Liu, B..Wang, P..Dong, X..Fernandez-Hernando, C..Li, Z. & Wu, D.(2008). Phospholipase C ${\beta}3$ deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice. The Journal of clinical investigation, 118(1), 195-204. https://doi.org/10.1172/JCI33139