Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Corni Fructus on Testosterone Deficiency Syndrome in In vitro and In vivo

In-vitro와 In-vivo에서 산수유의 남성갱년기 개선효과

  • Kim, Tae Muk (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jung, Ho Kyung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jang, Ji Hun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Sim, Mi Ok (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Lee, Mu Jin (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Jung Hee (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
  • 김태묵 ((재)한약진흥재단 한약자원본부) ;
  • 정호경 ((재)한약진흥재단 한약자원본부) ;
  • 장지훈 ((재)한약진흥재단 한약자원본부) ;
  • 심미옥 ((재)한약진흥재단 한약자원본부) ;
  • 이무진 ((재)한약진흥재단 한약자원본부) ;
  • 조정희 ((재)한약진흥재단 한약자원본부) ;
  • 조현우 ((재)한약진흥재단 한약자원본부)
  • Received : 2016.06.10
  • Accepted : 2016.08.26
  • Published : 2016.09.30
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Abstract

This study was carried out to evaluate the preventive effect of the Corni Fructus (SSU) 50 % EtOH extract (SSU-E50) against bisphenol A (BPA) toxicity in Leydig cells and improving testosterone deficiency syndrome in orchidectomized Sprague-Dawly (SD) rats. Antioxidant properties were measured by radical scavenging activity of SSU-E50 in ABTS assay and DPPH assay. Also, real-time polymerase chain reaction(real-time PCR) was performed to quantify the mRNA expression levels of antioxidant enzyme. SD rats were divided into eight group: normal, sham operation (Sham), orchidectomized (ORX), ORX treated with testosterone 1 mg/kg (Tes. 1), ORX treated with SSU water extract 100 mg/kg (SSU-A 100) and 300 mg/kg (SSU-A 300), ORX treated with SSU 50 % EtOH extract 100 mg/kg (SSU-E 100) and 300 mg/kg (SSU-E 300). On a comparative basis, the SSU showed better activity quenching ABTS with an IC50 value of 0.29 mg/ml and DPPH with an IC50 value of 0.33 mg/ml. Cell viability was evaluated by MTS assay as described not cytotoxic at the highest concentration of $500{\mu}g/ml$. Cytotoxicity of BPA showed in $200{\mu}M$, but definitely survived by treatment with SSU in Leydig cells. In addition, SSU increased the mRNA expression levels of antioxidant enzyme in BPA induced Leydig cells. Superoxide dismutase (SOD) level was slightly increased and malondialdehyde (MDA) level was decreased with SSU-A 100 in in-vivo. These results suggest that Corni Fructus extracts have the greatest property as a natural anti-oxidative and improves testosterone deficiency syndrome source.

Keywords

References

  1. Degen, G. H. and Bolt, H. M. (2000). Endocrine disruptors: Update on xenoestrogens. Int. Arch. Occup. Environ. Health 73: 433-441. https://doi.org/10.1007/s004200000163
  2. Kho, M. J., Bae, M. J. and Yang, M. (2010). Chemopreventive effects of Korean red ginseng on urinary bisphenol and malondialdehyde. Cancer Prev. Res. 15: 231-235.
  3. Zirkin, B. R. and Chen, H. (2000). Regulation of Leydig cell steroidogenic fuction during aging. Biol. Reprod. 63: 977-981. https://doi.org/10.1095/biolreprod63.4.977
  4. Patisaul, H. B. and Adewale, H. B. (2009). Long-term effects of environmental endocrine disruptors on reproductive physiology and behavior. Front Behav. Neurosci. 3: 10 (doi: 10.3389/neuro.08.010.2009).
  5. 이창복 (2003) 원색 대한식물도감. 향문사. 서울.
  6. Sreejayan, N. and Rao, M. N. A. (1996). Free radical scavenging activity of curcuminoids. Arzneimittel-Forschung 46: 169-171.
  7. Shirwaikar, A., Rajendra, K. and Kumar, C. D. (2004). In vitro antioxidant studies of Annona squamosal Linn. leaves. Indian J. Exp. Biol. 42: 803-807.
  8. Marklund, S. and Marklund, Gudrun. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient cssay for superoxide dismutase. Eur. J. Biochem. 47: 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  9. Chang, Y. F., Lee-Chang, J. S., Panneerdoss. S., MacLean, J. A. 2nd and Rao, M. K. (2011). Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis. BioTechniques 51: 341-344.
  10. Kim, K.-M. (2013) Late-Onset Hypogonadism. Korean J. Fam. Pract. 3: 245-254.
  11. Eo, J, and Lim, H. (2008). Effects of bisphenol A on gene expression and apoptosis of leydig cells in the mouse testis. Korean J, Reprod, Med. 35: 181-191.
  12. Mancini, A., Leone, E., Festa, R., Grande, G., Silvestrini, A. and de Marinis, L. (2008) Effects of testosterone on antioxidant systems in male secondary hypogonadism. J. Androl. 29: 622-629. https://doi.org/10.2164/jandrol.107.004838
  13. Gawel, S., Wardas, M., Niedworok, E. and Wardas, P. (2004) Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad. Lek. 57: 453-455.
  14. Lecomte, H. A. and Liggat, J. J. (2008) Commercial fireretarded PET formulation- relationship between thermal degradation behaviour and fire-retardant action. Polym. Deg. Stab. 93: 498-506. https://doi.org/10.1016/j.polymdegradstab.2007.11.005
  15. vom Saal, F. S., Cooke, P. S., Buchannan, D. L., Palanza, P., Thayer, K. A., Nagel, S. C., Parmigiani, S. and Welshons, W. V. (1998). A physiologically based approach to the study of bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production, and behaviour. Toxicol. Ind. Health. 14: 239-260. https://doi.org/10.1177/074823379801400115
  16. Iida H, Maehara K, Doiguchi M, Mori, T. and Tamada, F. (2003). Bisphenol A-induced apoptosis of cultured rat sertoli cells. Reprod. Toxicol. 17: 457-464. https://doi.org/10.1016/S0890-6238(03)00034-0
  17. Kim, J. H., Kim, J. K., Do, B. R., Lee, C. J. and Yoon, Y. D. (2005). Evaluation of bisphenol a induced apoptosis in sertoli cell-lines. Dev. Reprod. 9: 115-121.
  18. Xu, J., Osug,a Y., Yano, T., Morita, Y., Tang, X., Fujiwara, T., Takai, Y., Matsumi, H., Kog,a K., Taketani, Y. and Tsutsumi, O. (2002). Bisphenol A induces apoptosis and G2-to-M arrest of ovarian granulosa cells. Biochem. Biophys. Res. Commun. 292: 456-462. https://doi.org/10.1006/bbrc.2002.6644