Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis of Luteolin 5-glucoside in Ajuga spectabilis and Their Neuroprotective Effects

자란초에서 분리된 Luteolin 5-glucoside의 함량분석과 신경세포 보호 활성

  • Woo, Kyeong Wan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Sim, Mi Ok (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Kim, A Hyun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Kang, Byoung Man (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Jung, Ho Kyung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • An, Byeongkwan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Jung Hee (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
  • 우경완 (한약진흥재단 한약자원본부) ;
  • 심미옥 (한약진흥재단 한약자원본부) ;
  • 김아현 (한약진흥재단 한약자원본부) ;
  • 강병만 (한약진흥재단 한약자원본부) ;
  • 정호경 (한약진흥재단 한약자원본부) ;
  • 안병관 (한약진흥재단 한약자원본부) ;
  • 조정희 (한약진흥재단 한약자원본부) ;
  • 조현우 (한약진흥재단 한약자원본부)
  • Received : 2016.06.09
  • Accepted : 2016.08.16
  • Published : 2016.09.30
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Abstract

In the course of our continuing search for biologically active components from Korean medicinal plants, we isolated the main compound, luteolin 5-glucoside from aqueous fraction of Ajuga spectabilis. The structure was elucidated by the basis of $^1H$ and $^{13}C$ NMR and TOF ESI-MS data. Quantitative analysis of luteolin 5-glucoside was carried out on a XBridge C18 column ($S-5{\mu}m$, $4.6{\times}250mm$) with gradient elution composed of acetonitrile:water. The results exhibit that the average content of main compound in A. spectabilis were 0.048%. Oxidative stress plays a major role Alzheimer's disease (AD) and other neurodogenerative disease. AD is major health problem and there is currently no clinically accepted treatment to cure or stop its progression. Pretreatment with luteolin 5-glucoside markedly attenuated $H_2O_2$-induced cell viability loss in a dose-dependent manner. Luteolin 5-glucoside also inhibited the formation of intracellular reactive oxygen species in SH-SY5Y. The results suggest that luteolin 5-glucoside from A. spectabilis has protective effects against oxidative stress-induced cytotoxicity, which might be a potential therapeutic compound for treating and/or preventing neurodegenerative disease implicated with oxidative stress.

Keywords

References

  1. Collins, J. A., Wood, S. T., Nelson, K. J., Rowe, M. A., Carlson, C. S., Chubinskaya, S., Poole, L. B., Furdui, C. M. and Loeser, R. F. (2016) Oxidative stress promotes peroxiredoxin hyperoxidation and attenuates pro-survival signaling in aging chondrocytes. J. Biol. Chem. 291: 6641-6654. https://doi.org/10.1074/jbc.M115.693523
  2. Lee, S. O., Lee, H. J., Yu, M. H., Im, H. G. and Lee, I. S. (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol. 37: 233-240.
  3. Huang, S. H., Lin, C. M. and Chiang, B. H. (2008) Protective effects of Angelica sinensis extract on amyloid beta-peptideinduced neurotoxicity. Phytomedicine 15: 710-721. https://doi.org/10.1016/j.phymed.2008.02.022
  4. Sabogal-Guaqueta, A. M., Munoz-Manco, J. I., Ramirez-Pineda, J. R., Lamprea-Rodriguez, M., Osorio, E. and Cardona-Gomez, G. P. (2015) The flavonoid quercetin ameliorates alzheimer's disease pathology and protects cognitive and emotional function in aged triple transgenic alzheimer's disease model mice. Neuropharmacology 93: 134-145. https://doi.org/10.1016/j.neuropharm.2015.01.027
  5. Zhang, Y. C., Gan, F. F., Shelar, S. B., Ng, K. Y. and Chew, E. H. (2013) Antioxidant and Nrf2 inducing activities of luteolin, a flavonoid constituent in Ixeris sonchifolia Hance, provide neuroprotective effects against ischemia-induced cellular injury. Food Chem. Toxicol. 59: 272-280. https://doi.org/10.1016/j.fct.2013.05.058
  6. Jung, H. Y., Jin, S. E., Min, B. S., Kim, B. W. and Choi, J. S. (2012) Anti-inflammatory activity of Korean thistle Cirsium maackii and its major flavonoid, luteolin 5-O-glucoside. Food Chem. Toxicol. 50: 2171-2179. https://doi.org/10.1016/j.fct.2012.04.011
  7. Kumar, S., Chandra, P., Bajpai, V., Singh, A., Srivastava, M., Mishra, D. K. and Kumar, B. Rapid qualitative and quantitative analysis of bioactive compounds from Phyllanthus amarus using LC/MS/MS techniques. Ind. Crops. Prod. 69: 143-152.
  8. Woo, K. W., Han, J. Y., Suh, W. S., Lee, J. H. and Lee, K. R. (2014) Two new chemical constituents from leaves of Perilla frutescens var. acuta. Bull. Korean Chem. Soc. 35: 2151-2154. https://doi.org/10.5012/bkcs.2014.35.7.2151
  9. Chung, H. S. (2003) Inhibition of monamine oxidase by a flavone and its glycoside from Ixeris dentate Nakai. Nutraceuticals & Food. 8: 141-144.
  10. Zhao, B. (2009) Natural antioxidants protect neurons in alzheimer's disease and parkinson's disease. Neurochem Res. 34: 630-638. https://doi.org/10.1007/s11064-008-9900-9
  11. Kwon, S. H., Hong, S. I., Kim, J. A., Jung, Y. H., Kim, S. Y., Kim, H. C., Lee, S. Y. and Jang, C. G. (2011) The neuroprotective effects of Lonicera japonica THUNB. against hydrogen peroxide-induced apoptosis via phosphorylation of MAPKs and PI3K/Akt in SH-SY5Y cells. Food Chem. Toxicol. 49: 1101-1019.
  12. Zhang, Y. C., Gan, F. F., Shelar, S. B., Ng, K. Y. and Chew, E. H. (2013). Antioxidant and Nrf2 inducing activities of luteolin, a flavonoid constituent in Ixeris sonchifolia Hance, provide neuroprotective effects against ischemia -induced cellular injury. Food Chem. Toxicol. 59: 272-280. https://doi.org/10.1016/j.fct.2013.05.058