Clinical and Experimental Reproductive Medicine

  • 제43권3호
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  • Pages.152-156
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  • 2016
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

  • Sanmee, Usanee (Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University) ;
  • Piromlertamorn, Waraporn (Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University) ;
  • Vutyavanich, Teraporn (Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University)
  • 투고 : 2016.06.14
  • 심사 : 2016.08.09
  • 발행 : 2016.09.22
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초록

Objective: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. Methods: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). Results: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group ($69{\pm}19.3$, $74{\pm}15.7$, and $71{\pm}16.8$, respectively; p=0.680). Conclusion: These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers.

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참고문헌

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피인용 문헌

  1. Blastocyst hatching site is regularly distributed and does not influence foetal development in mice vol.10, pp.None, 2020, https://doi.org/10.1038/s41598-020-59424-2
  2. Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage vol.38, pp.2, 2016, https://doi.org/10.1007/s10815-020-01998-x