한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제39권3호
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  • Pages.159-166
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  • 2016
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
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육계로부터 분리한 병원성 세균에 대한 봉독의 항균효과

Antibacterial effects of purified bee venom against some pathogenic bacteria isolated from dead chickens

  • 한상미 (국립농업과학원 농업생물부) ;
  • 김세건 (국립농업과학원 농업생물부) ;
  • 홍인표 (국립농업과학원 농업생물부) ;
  • 우순옥 (국립농업과학원 농업생물부) ;
  • 장혜리 (국립농업과학원 농업생물부) ;
  • 이경우 (건국대학교 동물자원과학과)
  • Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Hong, In Phyo (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Lee, Kyung Woo (Department of Animal Science and Technology, Konkuk University)
  • 투고 : 2016.08.18
  • 심사 : 2016.09.30
  • 발행 : 2016.09.30
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초록

Clostridium perfringens, Salmonella thyphimurium and S. Montevideo isolated from the intestines of dead broiler chickens in Korea were tested for antibacterial effects to purifed bee venom. Purified bee venom from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. To investigate antibacterial effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). Purified bee venom exhibited significant inhibition of bacterial growth of C. perfringens, S. thyphimurium and S. Montevideo with MIC value of 0.85, 0.68 and $0.69{\mu}g/mL$, respectively. The MBC value of purified bee venom against C. perfringens, S. thyphimurium and S. Montevideo were 3.33, 2.66 and $2.86{\mu}g/mL$. Furthermore, the results of PAE values against C. perfringens, S. thyphimurium and S. Montevideo showed the bacterial effect with 3.5, 4.0 and 3.5 hr. Stability of pufifed bee venom at acidity from pH 1 to pH 8 for 24 hr was the antibacterial activity for C. perfringens, S. thyphimurium and S. Montevideo and melittin contents. Also purified bee venom processed through the heating for 15 min, there was no signification loss of the antibacterial activity and melittin at below $100^{\circ}C$. These results obtained in this study suggest that purified bee venom might be utilized as a feed additive in poultry diets.

키워드

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