Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Characteristics of Biocellulose by Gluconobacter uchimurae GYS15

Gluconobacter uchimurae GYS15 균주로부터 생산되는 Biocellulose의 특성 확인

  • 이영선 ((주)지에프씨 생명과학연구원) ;
  • 김재영 ((주)지에프씨 생명과학연구원) ;
  • 차미연 ((주)지에프씨 생명과학연구원) ;
  • 강희철 ((주)지에프씨 생명과학연구원)
  • Received : 2016.07.18
  • Accepted : 2016.09.22
  • Published : 2016.09.30
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Abstract

In order to select a strain that forms a Biocellulose (BC), strain producing acetic acid was selected from commercially available kombucha. Through SM broth it was confirmed that the strain is a gram negative bacteria in the form of rods having no motility through a phase contrast microscope. The result of phylogenetic inference analysis based on 16S rDNA sequence analysis for the identification of strains was most closely related to Gluconobacter uchimurae (G. uchimurae) and was named G. uchimurae GYS15 strain. The strain showed the highest degree of growth when cultured for 14 days under the conditions of pH 5 and $25^{\circ}C$. Moreover, it showed the highest degree of growth in a Glucose addition disaccharide as the optimum carbon source sucrose and fructose. Also, 0.5% NaCl, upon the addition of Malto extract, showed the highest degree of growth. Based on investigation by the optimum growth conditions to confirm the physical properties of BC obtained by culturing G. uchimurae GYS15 strains. The surface structure was observed through an scanning electron microscope (SEM) showed a high networks structure. It until $8.6{\pm}0.38$ times when the water holding capacity is re-absorbed and re-absorbed holding oil up to $6.6{\pm}0.51$ times confirmed. In conclusion, using these material properties, it was possible to confirm the possibility of a variety of cosmetic materials and mask pack materials.

화장품 소재로서 천연고분자 물질인 Biocellulose (BC)를 형성하는 균주를 선발하여 초산생성 균주를 선발하였다. SM 배지를 통하여 초산 생성 균주 1종을 선발하고 형태학적인 분석을 통하여 간균형태의 그람음성균임을 확인하였다. 또한 균주의 동정을 위하여 16S rDNA 유전자 분석을 통하여 계통도를 분석한 결과 Gluconobacter uchimurae (G. uchimurae)와 높은 상동성을 나타내어 G. uchimurae GYS15 균주로 명명하였다. 선발된 균주는 pH 5, $25^{\circ}C$ 조건의 환경적 요인에서 14일간 배양하였을 때 가장 높은 생육을 나타내었다. 또한 탄소원으로 glucose 이외에 이당류인 sucrose와 fructose를 첨가 하였을 때 가장 높은 생육활성을 보였고 최적의 염농도와 질소원은 0.5% NaCl과 malto extract로 확인하였다. 확인된 조건 하에서 G. uchimurae GYS15 균주를 배양하여 얻어진 BC의 물성을 확인하였다. 전자 주사현미경을 통하여 확인된 표면구조는 높은 표면적을 갖는 초미세망상구조로서 다공성 구조를 나타내었다. 이로 인해 수분의 경우 $8.6{\pm}0.38$배, 유분의 경우 $6.6{\pm}0.51$배까지 재흡수 되는 것으로 확인하였다. 따라서 BC의 이러한 구조적 특성을 이용하여 마스크팩 등의 다양한 화장품 소재로 활용 가능성을 확인하였다.

Keywords

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