Korean Chemical Engineering Research

  • 제54권5호
  • /
  • Pages.719-721
  • /
  • 2016
  • /
  • 0304-128X(pISSN)
  • /
  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
권호 표지
DOI QR코드

DOI QR Code

Formatotrophic Production of Poly-β-hydroxybutyric Acid (PHB) from Methylobacterium sp. using Formate as the Sole Carbon and Energy Source

  • Cho, Dae Haeng (Department of Chemical Engineering, Kwangwoon University) ;
  • Jang, Min Gee (Department of Chemical Engineering, Kwangwoon University) ;
  • Kim, Yong Hwan (Department of Chemical Engineering, Kwangwoon University)
  • 투고 : 2016.03.29
  • 심사 : 2016.08.17
  • 발행 : 2016.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Formate has been considered as an environmentally sustainable feedstock that can be used to accelerate the production of valuable chemicals. This study presents brief results of the formatotrophic production of Poly-${\beta}$-hydroxybutyric acid (PHB) by Methylobacterium sp. To evaluate the production of PHB, five species of Methylobacteria were tested using formate as the sole carbon and energy source. Methylobacterium chloromethanicum CM4 exhibited the highest productivity of PHB, which showed 1.72 g/L PHB production, 32.4% PHB content, and 0.027 g-PHB/g-formate PHB yield. These results could be used for the formatotrophic production of PHB with the concurrent reduction of $CO_2$ to formate.

키워드

참고문헌

  1. Whipple, D. T. and Kenis, P. J. A., "Prospects of $CO_2$ Utilization via Direct Heterogeneous Electrochemical Reduction," J. Phys. Chem. Lett., 1, 3451-3458(2010). https://doi.org/10.1021/jz1012627
  2. Enthaler, S., von Langermann, J. and Schmidt, T., "Carbon Dioxide and Formic Acid-the Couple for Environmental-friendly Hydrogen Storage?," Energy Environ. Sci., 3, 1207-1217(2010). https://doi.org/10.1039/b907569k
  3. Joo, F., "Breakthroughs in Hydrogen Storage-Formic Acid as a Sustainable Storage Material for Hydrogen," ChemSusChem., 1, 805-808(2008). https://doi.org/10.1002/cssc.200800133
  4. Thomas, M. C., Michelle, C. C., Lionel, C., Andrew, D. E., Richard, A. J., Jay, D. K., Stephen, S. L., Pilar, O., Kristala, L. J. P., Reshma, P. S., Christopher, A.V. and Humin, Z., Industrialization of Biology: A Roadmap to Accelerate the Advanced Manufacturing of Chemicals. National Academies Press. (2015).
  5. Kortlever, R., Peters, I., Koper, S. and Koper, M. T. M., "Electrochemical $CO_2$ Reduction to Formic Acid at Low Overpotential and with High Faradaic Efficiency on Carbon-Supported Bimetallic Pd-Pt Nanoparticles," ACS Catal., 5, 3916-3923(2015). https://doi.org/10.1021/acscatal.5b00602
  6. Hwang, H., Yeon, Y. J., Lee, S., Choe, H., Jang, M. G., Cho, D. H., Park, S. and Kim, Y. H., "Electro-biocatalytic Production of Formate from Carbon Dioxide Using an Oxygen-stable Whole Cell Biocatalyst," Bioresour. Technol., 185, 35-39(2015). https://doi.org/10.1016/j.biortech.2015.02.086
  7. Goldberg, I., Rock, J. S., Ben-Bassat, A. and Mateles, R. I., "Bacterial Yields on Methanol, Methylamine, Formaldehyde, and Formate," Biotechnol. Bioeng., 18, 1657-1668(1976). https://doi.org/10.1002/bit.260181202
  8. Large, P. J., Peel, D. and Quayle, J. R., "Microbial Growth on C(1) Compounds. 2. Synthesis of Cell Constituents by Methanoland Formate-grown Pseudomonas AM1, and methanol-grown Hyphomicrobium vulgare," Biochem. J., 81, 470-480(1961). https://doi.org/10.1042/bj0810470
  9. Bar-Even, A., Noor, E., Flamholz, A. and Milo, R., "Design and Analysis of Metabolic Pathways Supporting Formatotrophic Growth for Electricity-dependent Cultivation of Microbes," Biochim. Biophys. Acta, Bioenergetics., 1827, 1039-1047(2013). https://doi.org/10.1016/j.bbabio.2012.10.013
  10. Anthony, C. The Biochemistry of Methylotrophs. Academic Press. (1982).
  11. Choi, J. H., Kim, J. H., Daniel, M. and Lebeault, J. M., "Optimization of Growth Medium and Poly-${\beta}$-hydroxybutyric Acid Production from Methanol in Methylobacterium organophilum," Kor. J. Appl. Microbiol. Bioeng., 17, 392-396(1989).
  12. Braunegg, G., Sonnleitner, B. and Lafferty, R. M., "A Rapid Gas Chromatographic Method for the Determination of Poly-${\beta}$-hydroxybutyric Acid in Microbial Biomass," Eur. J. Appl. Microbio. Biotechnol., 6, 29-37(1978). https://doi.org/10.1007/BF00500854
  13. Bourque, D., Pomerleau, Y. and Groleau, D., "High-cell-density Production of Poly-${\beta}$-hydroxybutyrate (PHB) from Methanol by Methylobacterium extorquens: Production of High-molecular-mass PHB," Appl. Microbiol. Biotechnol., 44, 367-376(1995). https://doi.org/10.1007/BF00169931
  14. Kim, S., Kim, P., Lee, H. and Kim, J., "High production of Poly-${\beta}$-hydroxybutyrate (PHB) from Methylobacterium organophilum Under Potassium Limitation," Biotechnol Lett., 18, 25-30(1996). https://doi.org/10.1007/BF00137805
  15. Yamane, T., "Yield of Poly-d-3-hydroxybutyrate from Various Carbon Sources: a Theoretical Study," Biotechnol. Bioeng., 41, 165-170(1993). https://doi.org/10.1002/bit.260410122
  16. Crowther, G. J., Kosaly, G. and Lidstrom, M. E., "Formate as the Main Branch Point for Methylotrophic Metabolism in Methylobacterium extorquens AM1," J. Bacteriol., 190, 5057-5062(2008). https://doi.org/10.1128/JB.00228-08
  17. Chistoserdova, L., Crowther, G. J., Vorholt, J. A., Skovran, E., Portais, J.-C. and Lidstrom, M. E., "Identification of a Fourth Formate Dehydrogenase in Methylobacterium extorquens AM1 and Confirmation of the Essential Role of Formate Oxidation in Methylotrophy," J. Bacteriol., 189, 9076-9081(2007). https://doi.org/10.1128/JB.01229-07