Korean Circulation Journal

The Korean Society of Cardiology (대한심장학회)
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Isoproterenol Enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Induced Apoptosis in Human Embryonic Kidney Cells through Death Receptor 5 up-Regulation

  • Eom, Young Woo (Cell Therapy and Tissue Engineering Center, Wonju College of Medicine, Yonsei University) ;
  • Jung, Ha Yun (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Oh, Ji-Eun (Cell Therapy and Tissue Engineering Center, Wonju College of Medicine, Yonsei University) ;
  • Lee, Jun-Won (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Ahn, Min-Soo (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Youn, Young Jin (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Ahn, Sung Gyun (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Kim, Jang Young (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Lee, Seung-Hwan (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Yoon, Junghan (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University) ;
  • Yoo, Byung-Su (Cardiology Division, Department of Internal Medicine, Wonju College of Medicine, Yonsei University)
  • Received : 2015.04.12
  • Accepted : 2015.07.07
  • Published : 2016.01.30
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Abstract

Background and Objectives: Chronic impairment of ${\beta}$-adrenergic receptor signaling increases cardiac apoptosis, hypertrophy and fibrosis. The aim of this study was to investigate whether isoproterenol (ISO), an agonist of the adrenergic receptor, can enhance tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human embryonic kidney (HEK) 293 cells. Materials and Methods: HEK 293 cells were treated with ISO and/or TRAIL for 24 hours. Cell viability was evaluated by microscopy and an established viability assay, and apoptotic cell death was analyzed by staining with fluorescein isothiocynate-annexin-V/propidium iodide (PI) and caspase activation. To confirm the mechanism of cell death induced by co-treatment with ISO and TRAIL, expression of TRAIL receptor 2 (death receptor 5, DR5) was evaluated by immunoblotting. Results: Although ISO or TRAIL treatment decreased HEK 293 cell viability by 13% and 17%, respectively, co-treatment with ISO and TRAIL resulted in a markedly higher death rate of 35% after 24 hours. Increases were evident in early apoptotic cells (i.e., annexin-V positive/PI negative; 19.4%), late apoptotic cells (i.e., annexin-V positive/PI positive; 6.3%) and dead cells (i.e., annexin-V negative/PI positive; 1.1%) when cells were co-treated with ISO and TRAIL, compared to cells treated with either ISO or TRAIL. In addition, marked increases of cleaved cas-3, cleaved poly (adenosine diphosphate-ribose) polymerase and DR5 were observed in HEK 293 cells co-treated with ISO and TRAIL. Conclusion: Treatments combining ISO with TRAIL may be responsible for death of HEK 293 cells through DR5 up-regulation. Activation of adrenergic receptors is responsible for the synergistic cell death observed with TRAIL.

Keywords

Acknowledgement

Supported by : Korean Society of Cardiology

References

  1. Whelan RS, Kaplinskiy V, Kitsis RN. Cell death in the pathogenesis of heart disease: mechanisms and significance. Annu Rev Physiol 2010;72:19-44. https://doi.org/10.1146/annurev.physiol.010908.163111
  2. Zauli G, Secchiero P. The role of the TRAIL/TRAIL receptors system in hematopoiesis and endothelial cell biology. Cytokine Growth Factor Rev 2006;17:245-57. https://doi.org/10.1016/j.cytogfr.2006.04.002
  3. Ashkenazi A, Dixit VM. Apoptosis control by death and decoy receptors. Curr Opin Cell Biol 1999;11:255-60. https://doi.org/10.1016/S0955-0674(99)80034-9
  4. Galligan L, Longley DB, McEwan M, Wilson TR, McLaughlin K, Johnston PG. Chemotherapy and TRAIL-mediated colon cancer cell death: the roles of p53, TRAIL receptors, and c-FLIP. Mol Cancer Ther 2005;4:2026-36. https://doi.org/10.1158/1535-7163.MCT-05-0262
  5. Kim Y, Seol DW. TRAIL, a mighty apoptosis inducer. Mol Cells 2003;15:283-93.
  6. Ashkenazi A. Targeting death and decoy receptors of the tumour-necrosis factor superfamily. Nat Rev Cancer 2002;2:420-30. https://doi.org/10.1038/nrc821
  7. Wilson NS, Dixit V, Ashkenazi A. Death receptor signal transducers: nodes of coordination in immune signaling networks. Nat Immunol 2009;10:348-55. https://doi.org/10.1038/ni.1714
  8. Yamaguchi H, Wang HG. CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells. J Biol Chem 2004;279:45495-502. https://doi.org/10.1074/jbc.M406933200
  9. Naga Prasad SV, Nienaber J, Rockman HA. Beta-adrenergic axis and heart disease. Trends Genet 2001;17:S44-9. https://doi.org/10.1016/S0168-9525(01)02487-8
  10. Engelhardt S, Hein L, Wiesmann F, Lohse MJ. Progressive hypertrophy and heart failure in beta1-adrenergic receptor transgenic mice. Proc Natl Acad Sci U S A 1999;96:7059-64. https://doi.org/10.1073/pnas.96.12.7059
  11. Houser SR, Margulies KB, Murphy AM, et al. Animal models of heart failure: a scientific statement from the American Heart Association. Circ Res 2012;111:131-50. https://doi.org/10.1161/RES.0b013e3182582523
  12. Communal C, Singh K, Pimentel DR, Colucci WS. Norepinephrine stimulates apoptosis in adult rat ventricular myocytes by activation of the beta-adrenergic pathway. Circulation 1998;98:1329-34. https://doi.org/10.1161/01.CIR.98.13.1329
  13. Zaugg M, Xu W, Lucchinetti E, Shafiq SA, Jamali NZ, Siddiqui MA. Beta-adrenergic receptor subtypes differentially affect apoptosis in adult rat ventricular myocytes. Circulation 2000;102:344-50. https://doi.org/10.1161/01.CIR.102.3.344
  14. Liu JJ, Li DL, Zhou J, et al. Acetylcholine prevents angiotensin IIinduced oxidative stress and apoptosis in H9c2 cells. Apoptosis 2011;16:94-103. https://doi.org/10.1007/s10495-010-0549-x
  15. Groenning BA, Nilsson JC, Sondergaard L, Fritz-Hansen T, Larsson HB, Hildebrandt PR. Antiremodeling effects on the left ventricle during beta-blockade with metoprolol in the treatment of chronic heart failure. J Am Coll Cardiol 2000;36:2072-80. https://doi.org/10.1016/S0735-1097(00)01006-8
  16. Park JB, Lee JS, Cho BP, et al. Adipose tissue-derived mesenchymal stem cells cultured at high cell density express brain-derived neurotrophic factor and exert neuroprotective effects in a 6-hydroxydopamine rat model of Parkinson's disease. Genes Genom 2015;37:213-21. https://doi.org/10.1007/s13258-014-0239-0
  17. Shetty S, Gladden JB, Henson ES, et al. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) up-regulates death receptor 5 (DR5) mediated by NFkappaB activation in epithelial derived cell lines. Apoptosis 2002;7:413-20. https://doi.org/10.1023/A:1020031023947
  18. Fanger NA, Maliszewski CR, Schooley K, Griffith TS. Human dendritic cells mediate cellular apoptosis via tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). J Exp Med 1999;190:1155-64. https://doi.org/10.1084/jem.190.8.1155
  19. Griffith TS, Wiley SR, Kubin MZ, Sedger LM, Maliszewski CR, Fanger NA. Monocyte-mediated tumoricidal activity via the tumor necrosis factor-related cytokine, TRAIL. J Exp Med 1999;189:1343-54. https://doi.org/10.1084/jem.189.8.1343
  20. Kemp TJ, Moore JM, Griffith TS. Human B cells express functional TRAIL/Apo-2 ligand after CpG-containing oligodeoxynucleotide stimulation. J Immunol 2004;173:892-9. https://doi.org/10.4049/jimmunol.173.2.892
  21. Zamai L, Ahmad M, Bennett IM, Azzoni L, Alnemri ES, Perussia B. Natural killer (NK) cell-mediated cytotoxicity: differential use of TRAIL and Fas ligand by immature and mature primary human NK cells. J Exp Med 1998;188:2375-80. https://doi.org/10.1084/jem.188.12.2375
  22. Cassatella MA, Huber V, Calzetti F, et al. Interferon-activated neutrophils store a TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) intracellular pool that is readily mobilizable following exposure to proinflammatory mediators. J Leukoc Biol 2006;79:123-32. https://doi.org/10.1189/jlb.0805431
  23. Koga Y, Matsuzaki A, Suminoe A, Hattori H, Hara T. Neutrophil-derived TNF-related apoptosis-inducing ligand (TRAIL): a novel mechanism of antitumor effect by neutrophils. Cancer Res 2004;64:1037-43. https://doi.org/10.1158/0008-5472.CAN-03-1808
  24. Kemp TJ, Ludwig AT, Earel JK, et al. Neutrophil stimulation with Mycobacterium bovis bacillus Calmette-Guerin (BCG) results in the release of functional soluble TRAIL/Apo-2L. Blood 2005;106:3474-82. https://doi.org/10.1182/blood-2005-03-1327
  25. Simons MP, Leidal KG, Nauseef WM, Griffith TS. TNF-related apoptosis-inducing ligand (TRAIL) is expressed throughout myeloid development, resulting in a broad distribution among neutrophil granules. J Leukoc Biol 2008;83:621-9. https://doi.org/10.1189/jlb.0707452
  26. Murray DR, Prabhu SD, Chandrasekar B. Chronic beta-adrenergic stimulation induces myocardial proinflammatory cytokine expression. Circulation 2000;101:2338-41. https://doi.org/10.1161/01.CIR.101.20.2338
  27. Liao X, Wang X, Gu Y, Chen Q, Chen LY. Involvement of death receptor signaling in mechanical stretch-induced cardiomyocyte apoptosis. Life Sci 2005;77:160-74. https://doi.org/10.1016/j.lfs.2004.11.029
  28. Prasad S, Kim JH, Gupta SC, Aggarwal BB. Targeting death receptors for TRAIL by agents designed by Mother Nature. Trends Pharmacol Sci 2014;35:520-36. https://doi.org/10.1016/j.tips.2014.07.004
  29. Lee MW, Park SC, Kim JH, et al. The involvement of oxidative stress in tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in HeLa cells. Cancer Lett 2002;182:75-82. https://doi.org/10.1016/S0304-3835(02)00074-5
  30. Chandel NS, Schumacker PT, Arch RH. Reactive oxygen species are downstream products of TRAF-mediated signal transduction. J Biol Chem 2001;276:42728-36. https://doi.org/10.1074/jbc.M103074200

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