The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Evaluation of cellular energy allocation (CEA) in the Manila clam, Ruditapes philippinarum as a tool for assessment of contaminated sediments

오염 퇴적물 평가 기법으로서의 바지락 (Ruditapes philippinarum) 세포내 에너지 할당 (cellular energy allocation, CEA) 적용성 검토

  • Sung, Chan-Gyoung (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.) ;
  • Kang, Sin-Kil (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.) ;
  • Chung, Jiwoong (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.) ;
  • Park, Dong-Ho (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.) ;
  • Lee, Jong-Hyeon (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.) ;
  • Lee, Chang-Hoon (Environmental Health & Safety Research Institute, EH R&C Co. Ltd.)
  • 성찬경 (이에이치알앤씨(주) 환경보건안전연구소) ;
  • 강신길 (이에이치알앤씨(주) 환경보건안전연구소) ;
  • 정지웅 (이에이치알앤씨(주) 환경보건안전연구소) ;
  • 박동호 (이에이치알앤씨(주) 환경보건안전연구소) ;
  • 이종현 (이에이치알앤씨(주) 환경보건안전연구소) ;
  • 이창훈 (이에이치알앤씨(주) 환경보건안전연구소)
  • Received : 2016.03.07
  • Accepted : 2016.03.31
  • Published : 2016.03.31
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Abstract

To evaluate the applicability of cellular energy allocation (CEA) in the bivalves as a biomarker for the assessment of environmental contamination, the energy contents and energy consumption in several tissues of the Manila clam, Ruditapes philippinarum were analyzed. The contents of lipid, glucose, protein and electron transport system (ETS) activity in the foot, siphons, gills, and body of R. philippinarum exposed to crude oil-spiked sediments were measured at 1, 2, 4, 7, 10 days after exposure. The reserved energy (energy available, EA) in the lipid, glucose and protein decreased as contamination level and exposure time increased. In contrast, the ETS activity (energy consumed, EC) showed the reverse tendency. The order of available energy contents were foot > siphons > gill > body. Significant differences in both EA and EC were found only at the highest contamination level (58.3 mg TPAHs/kg DW). EA decreased significantly in the foot and gill at 1 day, in the body at 2 and 7 days after exposure. EC increased significantly in the body at 4 days after exposure. CEA showed higher sensitivity to the contamination than EA or EC. Especially, CEA in the foot and body decreased significantly at lower ranges of contamination level (as low as 6.5 mg TPAHs/kg DW) during 1 to 7 days after exposure. The CEA is more useful than EA or EC alone for the assessment of sediment contamination at lower level that acute toxicity could not be detected. CEA analyses in the body of R. philippinarum after 4 days' exposure to contaminated sediments seem to be the most sensitive and reliable.

환경오염 평가를 위한 생물지표로 세포내 에너지할당(CEA) 의 적용성을 검토하기 위해 바지락 (Ruditapes philippinarum) 조직을 대상으로 에너지 함량과 에너지 소비율을 분석하였다. 원유를 인위적으로 오염시킨 퇴적물에 바지락을 1, 2, 4, 7, 10 일간 노출 후 일부 조직 (발, 입출수공, 아가미 그리고 몸통) 내 지질, 글루코오스, 단백질 함량, 그리고 전자전달계 (electron transport system, ETS) 활성을 측정하였다. 바지락의 체내 지질, 글루코오스, 단백질 에너지 함량(energy available, EA)은 오염의 수준과 노출시간이 증가함에 따라 감소하였으나, 이와 대조적으로 ETS 활성은 그 반대의 경향을 보였다. 바지락의 조직별 EA는 발 > 입출수공 > 아가미 > 몸통 순이었다. EA와 EC의 분석에서 통계적으로 유의한 차이는 노출 최고 농도 (58.3 mg TPAHs/kg DW) 에서만 발견되었다. 노출 1 일째 발과 아가미에서, 노출 2, 7 일째 몸통에서 EA가 감소하였고, 노출 4 일째 몸통에서 EC가 증가하였다. CEA는 EA 또는 EC 보다 오염에 더 민감하게 반응하였다. 특히, 노출 1-7 일 기간 동안 몸통에서 CEA는 오염 수준이 낮은 (6.5 mg TPAHs/kg DW) 범위에서도 크게 감소하였다. CEA는 급성독성이 나타나지 않는 낮은 수준의 퇴적물오염 평가에서 EA 또는 EC보다 더 민감하였다. 바지락을 이용한 오염 퇴적물 평가시 노출은 4 일간, 그리고 몸통부위에서 CEA를 측정하는 것이 가장 민감하고 신뢰할 수 있을 것으로 여겨진다.

Keywords

References

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