한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제24권4호
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  • Pages.309-316
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  • 2016
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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아로니아 저온 초음파 추출물의 항비만 활성 증진

Enhancement of Anti-Obesity Activities of Aronia melanocarpa Elliot Extracts from Low Temperature Ultrasonification Process

  • 김남영 (강원대학교 생물의소재공학과) ;
  • 이정민 (한림대학교 의과대학 생화학교실) ;
  • 이재용 (한림대학교 의과대학 생화학교실) ;
  • 이현용 (서원대학교 식품공학과)
  • Kim, Nam Young (Department of Medical Biomaterials Engineering, Kangwon National University) ;
  • Lee, Jeong Min (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Jae Yong (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Hyeon Yong (Department of Food Science and Engineering, Seowon University)
  • 투고 : 2016.04.20
  • 심사 : 2016.07.15
  • 발행 : 2016.08.30
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초록

Background: This study represents the first report that the anti-obesity activity of ethanol extracts of Aronia melanocarpa can be enhanced through ultrasonification at a frequency of 120 kHz at $60^{\circ}C$ (UE). Methods and Results: The amounts of cyanidin-3-O-galactose (cya-gal), a major anthocyanin in A. melanocarpa were higher by up to 402.4 mg/100 g, as compared with 221.4 mg/100 g and 322.1 mg/100 g, for hot water at $100^{\circ}C$ and 70% ethanol at $80^{\circ}C$ respectively. This result should cause the higher antioxidant activities of the UE than extract of hot water and ethanol in DPPH free radical scavenging. It was confirmed that the high antioxidant activity of UE could play an important role in inhibiting the production of proteins related to adipocyte differentiation, such as peroxisome proliferator activated receptor-${\gamma}$ (PPAR-${\gamma}$) and sterol regulatory element binding protein 1 (SREBP1). Conclusions: Ultrasonification at a frequency of 120 kHz at $60^{\circ}C$ should result in better anti-obesity activity than that observed using other processes. It was also observed for the first time that the anti-obesity activity of A. melanocarpa was associated with its antioxidant activity, possibly due to the higher elution of intact cya-gal, owing to efficient low temperature ultrasonification extraction. These results could also be applied to improve other biological activities of medicinal herbs that contain many types of heat-labile bioactive substances.

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참고문헌

  1. Attie AD and Scherer PE. (2009). Adipocyte metabolism and obesity. The Journal of Lipid Research. 50:395-399. https://doi.org/10.1194/jlr.R800057-JLR200
  2. Barstard JP, Maachi M, Lagathu C, Kim MJ, Caron M, Vidal H, Capeau J and Feve B. (2006). Recent advances in the relationship between obesity, inflammation and insulin resistance. European Cytokine Network. 17:4-12.
  3. Brunlich M, Slimestad R, Wangensteen H, Brede C, Malterud KE and Barsett H. (2013). Extracts, anthocyanins and procyanidins from Aronia melanocarpa as radical scavengers and enzyme inhibitors. Nutrients. 5:663-678. https://doi.org/10.3390/nu5030663
  4. Camp HS, Ren D and Leff T. (2002). Adipogenesis and fat-cell function in obesity and diabetes. Trends in Molecular Medicine. 8:442-447. https://doi.org/10.1016/S1471-4914(02)02396-1
  5. Choi HM, Moon SO, Lee HH and Lee HD. (2015). Inhibitory effect of by Ojeok-san lipid accumulation in high fat dietinduced obesity mice and 3T3-L1 adipocytes. The Korea Journal of Herbology. 30:121-128. https://doi.org/10.6116/kjh.2015.30.4.121.
  6. Chung KW, Kim WI, Hong IK and Park KA. (2000). Ultrasonic energy effects on squalene extraction from amaranth seed. Applied Chemistry. 4:149-152.
  7. d'Alessandro LG, Kriaa K, Nikov I and Dimitrov K. (2012). Ultrasound assisted extraction of polyphenols from black chokeberry. Separation and Purification Technology. 93:42-47. https://doi.org/10.1016/j.seppur.2012.03.024
  8. Dietz BM, Kang YH, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF, Farnsworth NR, Mesecar AD, van Breemen RB and Bolton JL. (2005). Xanthohumol isolated from Humulus lupulus inhibits menadione-induced DNA damage through induction of quinone reductase. Chemical Research in Toxicology. 18:1296-1305. https://doi.org/10.1021/tx050058x
  9. Horton JD, Goldstein JL and Brown MS. (2002). SREBPs: Transcriptional mediators of lipid homeostasis. Cold Spring Harbor Symposia on Quantitative Biology. 67:491-498. https://doi.org/10.1101/sqb.2002.67.491
  10. Huh EJ, Shin HK, Choi SY, Kwon OG and Lee YR. (2008). Thermosusceptible developmental stage in anthocyanin accumulation and color response to high temperature in red chrysanthemum cultivars. Korean Journal of Horticultural Science and Technology. 26:357-361.
  11. Hwang YP, Choi JH, Han EH, Kim HG, Wee JH, Jung KO, Jung KH, Kwon KI, Jeong TC, Chung YC and Jeong HG. (2011). Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphateactivated protein kinase in human HepG2 cells and obese mice. Nutrition Research. 31:896-906. https://doi.org/10.1016/j.nutres.2011.09.026
  12. Jayaprakasam B, Olson LK, Schutzki RE, Tai MH and Nair MG. (2006). Amelioration of obesity and glucose intolerance in high-fat-fed C57BL/6 mice by anthocyanins and ursolic acid in Cornelian cherry(Cornus mas). Journal of Agricultural and Food Chemistry. 54:243-248. https://doi.org/10.1021/jf0520342
  13. Kao ES, Tseng TH, Lee HJ, Chan KC and Wang CJ. (2009). Anthocyanin extracted from Hibiscus attenuate oxidized LDLmediated foam cell formation involving regulation of CD36 gene. Chemico-Biological Interactions. 179:212-218. https://doi.org/10.1016/j.cbi.2009.01.009
  14. Kim HG, Kim GW, Oh H, Yoo SY, Kim YO and Oh MS. (2011). Influence of roasting on the antioxidant activity of small black soybean(Glycine max L. Merrill). Food Science and Technology. 44:992-998.
  15. Kim JB and Park JY. (2002). Molecular insights into fat cell differentiation and functional roles of adipocytokines. Journal of Korean Society of Endocrinology. 17:1-9.
  16. Kim NY, Kim JH, Choi GP and Lee HY. (2014). Comparison of anti-skin wrinkle activities of Aronia melanocarpa extracts by extraction methods. Korean Journal of Medicinal Crop Science. 22:217-222. https://doi.org/10.7783/KJMCS.2014.22.3.217
  17. Lee MK, Liu Q, Hwang BY, Kim SY and Lee JH. (2011). Inhibitory effect of eggplant extract on adipocyte differentiation in 3T3-L1 cells. Yakhak Hoeji. 55:309-313.
  18. Lee OH, Kwon YI, Hong HD, Park CS, Lee BY and Kim YC. (2009). Production of reactive oxygen species and changes in antioxidant enzyme acitvities during differentiation of 3T3-L1 adipocyte. Journal of the Korean Society for Applied Biological Chemistry. 52:70-75.
  19. Li H and Jeong JM. (2015). Antioxidant activities of various berries ethanolic extract. Korean Journal of Medicinal Crop Science. 23:49-56. https://doi.org/10.7783/KJMCS.2015.23.1.49
  20. Lowell BB and Spiegelman BM. (2000). Towards a molecular understanding of adaptive thermogenesis. Nature. 404:652-660. https://doi.org/10.1038/35007527
  21. Ohgami K, Ilieva I, Shiratori K, Koyama Y, Jin XH, Yoshida K, Kase S, Kitaichi N, Suzuki Y, Tanaka T and Ohno S. (2005). Anti-inflammatory effect of aronia extract on rat endotoxininduced uveitis. Investigative Ophthalmology and Visual Science. 46:275-281. https://doi.org/10.1167/iovs.04-0715
  22. Oszmiaski J and Wojdylo A. (2005). Aronia melanocarpa phenolics and their antioxidant activity. European Food Research and Technology. 221:809-813. https://doi.org/10.1007/s00217-005-0002-5
  23. Park SJ, Choi JH, Jung YS and Yu MH. (2013). Inhibitory effect of Rumex crispus L. fraction on adipocyte differentiation in 3T3-L1 cells. Korean Journal of Food Science and Technology. 45:90-96. https://doi.org/10.9721/KJFST.2013.45.1.90
  24. Rayalam S, Della-Fera MA and Baile CA. (2008). Phytochemicals and regulation of the adipocyte life cycle. The Journal of Nutritional Biochemistry. 19:717-726. https://doi.org/10.1016/j.jnutbio.2007.12.007
  25. Rosen ED and Spiegelman BM. (2006). Adipocytes as regulators of energy balance and glucose homeostasis. Nature. 444:847-853. https://doi.org/10.1038/nature05483
  26. Slimestad R, Torskangerpoll K, Nateland HS, Johannessen T and Giske NH. (2005). Flavonoids from black chokeberries, Aronia melanocarpa. Journal of Food Composition and Analysis. 18:61-68. https://doi.org/10.1016/j.jfca.2003.12.003
  27. Spielgelman BM and Enerback S. (2006). The adipocyte: A multifunctional cell. Cell Metabolism. 4:425-427. https://doi.org/10.1016/j.cmet.2006.11.009
  28. Tanaka T and Tanaka A. (2001). Chemical components and characteristics of black chokeberry. Journal of the Japanese Society for Food Science and Technology. 48:606-610. https://doi.org/10.3136/nskkk.48.606
  29. Valko M, Libfritz D, Moncol J, Cronin MTD, Mazur M and Telser J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology. 39:44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  30. Wang H, Cao G and Prior RL. (1997). Oxygen radical absorbing capacity of anthocyanins. Journal of Agricultural and Food Chemistry. 45:304-309. https://doi.org/10.1021/jf960421t
  31. Yoo JS, Ku JR, Yoon KH, Jo JH, Jang DH, Jung YS, Kim JH, Kim BC, Seok HJ, Yoon MC, Roh JS and Shin SS. (2015). Comparison of Gangji-hwan-1,2,3,4 and combination of Gangjihwan-1 and Gamisoche-hwan in the reducing effects of body weight in a high fat diet-fed obese mice. Journal of Society of Korean Medicine for Obesity Research. 15:9-23. https://doi.org/10.15429/jkomor.2015.15.1.9
  32. Yoo SJ. (2008). Pharmacological treatment of obesity. Journal of Korean Endocrin Society. 23:223-233. https://doi.org/10.3803/jkes.2008.23.4.223

피인용 문헌

  1. Cyanidine-3-O-Galactoside Enriched <i>Aronia melanocarpa</i> Extract Inhibits Adipogenesis and Lipogenesis via Down-Regulation of Adipogenic Transcription Factors and Their Target Genes in 3T3-L1 Cells vol.10, pp.02, 2019, https://doi.org/10.4236/fns.2019.102011