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돌기해삼(Apostichopus japonicus)의 보관 후 회복 가능성 파악을 위한 외상 및 형태학적 변화의 관찰

Observation of External Injury and Morphological Movement for Analysis of Recovery Possibility after Storage of the Juvenile Sea Cucumber, Apostichopus japonicus

  • 김태익 (국립수산과학원 남해수산연구소) ;
  • 손맹현 (국립수산과학원 수산방역과) ;
  • 조재권 (국립수산과학원 양식관리과) ;
  • 진영국 (국립수산과학원 남해수산연구소)
  • 투고 : 2016.05.11
  • 심사 : 2016.06.30
  • 발행 : 2016.08.31

초록

돌기해삼 종묘의 보관에 따른 외상, 부위별 움직임 및 생존율을 관찰하였다. 10, 15, 20 및 $25^{\circ}C$에 종묘를 3, 6, 12, 24시간 보관 후 회복과정을 통해 생존율을 관찰하였고, 이를 위해 외상정도와 네 가지 부위별 움직임 변화(촉수 움직임, 촉수를 제외한 몸의 움직임, 뒤집기, 이동)를 적용할 수 있는지 검증하였다. 외상정도는 $20^{\circ}C$에서 24시간 실험구와 $25^{\circ}C$의 12시간 실험구에서 각각 $2.2{\pm}0.8$, $2.6{\pm}0.7$로 낮게 나타났다. 부위별 움직임 변화는 촉수의 움직임과 이동 여부 항목에서 생존율 및 외상 정도와 관련성을 보였다. 생존율은 보관 온도 $10^{\circ}C$$15^{\circ}C$ 실험구에서는 24시간까지, $20^{\circ}C$는 12시간까지 그리고 $25^{\circ}C$에서 6시간까지는 변화가 없었다. 돌기해삼 종묘의 보관 온도에 따른 치사시간(LT10)은 $20^{\circ}C$의 경우 15.73시간 이었으며, $25^{\circ}C$의 경우 5.57시간 이었다. 본 연구결과는 방류종묘의 운송에 따른 생존율을 간접적으로 파악할 수 있는 지표로서 적용 가능할 것이다.

The observed external injury, movement and survival rate according to storage and recovery of the juvenile sea cucumber, Apostichopus japonicus(wet weight $1.0{\pm}0.2g$). The investigated application possibility of external injury(grade I~IV) and movement variation(buccal tentacle, motion, obversion, movement) for estimate of survival rate. The survival rate was observed through the recovery after storage of the sea cucumber in various water temperature(10, 15, 20, $25^{\circ}C$) and hours(3, 6, 12, 24 hour). Grade of external injury lower in the 24 hour experiment group of $20^{\circ}C$ water temperature and 12 hour experiment group of $25^{\circ}C$ water temperature. Buccal tentacle and movement strong related survival rate and external injury. In the case of $20^{\circ}C$ water temperature, survival rate was observed decrease 24 hours experiment. $25^{\circ}C$ water temperature was high to 6 hours but 12 hours experiment group observed decrease of the survival rate. The lethal time(LT10) of the juvenile sea cucumber was 15.73 hours($20^{\circ}C$) and 5.57 hours($25^{\circ}C$). The results of this study provided various measurement method of survival rate according to transportation of the juvenile sea cucumber for release.

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참고문헌

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