DOI QR코드

DOI QR Code

TDN/CP 비율이 젖소 12~14개월령 육성우 영양소 섭취량, 증체량 및 혈액성상에 미치는 영향

Effects of TDN/CP Ratio on Nutrient Intake, Body Weight Gain, Blood Characteristics of Dairy Heifers from 12 to 14 Months of Age

  • 김겸헌 (건국대학교 동물자원과학과) ;
  • 황원욱 (건국대학교 동물자원과학과) ;
  • 김현진 (서울대학교 농업생명과학연구원) ;
  • 김수기 (건국대학교 동물자원과학과)
  • Kim, Gyeom-Heon (Department of Animal Science and Technology, Konkuk University) ;
  • Hwang, Won-Uk (Department of Animal Science and Technology, Konkuk University) ;
  • Kim, Hyun-Jin (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
  • 투고 : 2016.04.29
  • 심사 : 2016.05.18
  • 발행 : 2016.06.30

초록

본 연구는 수정 전 단계인 생후 12~14개월령의 육성우에게 급여하는 사료의 적정 영양소 수준에 대해 알아보고자 에너지와 단백질의 비율에 따른 영양소 이용성 및 증체량에 미치는 영향을 평가하였다. 건물 섭취량은 T1 (TDN/CP 비율, 5.0:1), T2 (TDN/CP 비율, 4.5:1) 그리고 T3 (TDN/CP 비율, 4.0:1)가 비슷한 수치를 보였다. 분 중 회분은 T1이 11.07%로 T3 (9.69%) 보다 유의적으로 높아졌으며, TDN/CP 비율이 높아질수록 분 내 회분 함량도 증가되었다. 초기 체중과 상관없이 증체량은 실험 개시 후 30일째 사료 내 CP 비율이 높아질수록 유의적으로 증가하였으나 60일째에는 T2가 유의적으로 가장 높은 증체를 보였다 (p<0.05). TDN 비율이 감소하거나 단백질 비율이 높아질수록 증체량은 유의적으로 증가되었다(p<0.05). 실험 종료 시의 혈중 GPT는 T1과 T3 (112.6, 88.3 u/l)가 T2 (50.9 u/l) 보다 유의적으로 높은 수치를 나타냈으며, phosphorus는 T1 (46.3 mg/dl)이 다른 처리구보다 유의적으로 높은 값을 보였다(p<0.05). HDL cholesterol은 T2 (145.2 md/dl)가 T1 (121.0 mg/dl)과 T3 (132.3 mg/dl) 보다 유의적으로 높았으며, triglyceride는 T3 (40.6 mg/dl)가 T1, T2 (20.7, 29.0 mg/dl) 보다 유의적으로 높은 수치를 보였다(p<0.05). BUN 농도를 비롯한 그 외의 혈액 분석항목들에서는 처리구별에 있어서 유의적 차이가 없었다. 결과적으로 섭취량과 증체율에서는 TDN/CP 비율이 4:1이 가장 좋았으나 체지방 축적이 과잉될 가능성이나 혈액대사 수치를 함께 고려한다면 이 시기에서는 4.5:1의 비율이 바람직한 것으로 판단된다.

In this study, the effect of energy and protein ratio on the nutrient availability and body weight gain was determined to find the proper nutrient level for the stage of replacement dairy heifers before fertilization at approximately 12~24 months old after birth. Dry matter intake showed that the TDN/CP rate of T1 (5.0:1), T2 (4.5:1) and T3 (4.0:1) resulted in similar values. Among feces, the ash content of T1 was 11.07%, which was significantly (p < 0.05) higher than that of T3 (9.69%). It was also significantly (p < 0.05) higher than that of T2 (10.32%). When the TDN/CP rate was increased, the ash content in the feces was also increased. Regardless of the initial weight, weight gain was increased significantly as the CP rate in the feed was increased on the $30^{th}$ day. On the $60^{th}$ day, T2 showed the highest rate of gain (p < 0.05). As the TDN rate was decreased or the protein rate was increased, weight gain was significantly (p < 0.05) increased. Regarding GPT level in the blood after finishing the test, T1 and T3 (112.6, 88.3 u/l) showed significantly (p < 0.05) higher value than T2 (50.9 u/l). For phosphorus level, T1 (46.3 mg/dl) showed significantly (p < 0.05) higher value than other treatments. For HDL cholesterol level, T2 (145.2 mg/dl) had significantly (p < 0.05) higher level than T1 (121.0 mg/dl) or T3 (132.3 mg/dl). For triglyceride levels, T3 (40.6 mg/dl) had significantly (p < 0.05) higher value than T1 (20.7 mg/dl) and T2 (29.0 mg/dl). For other blood parameters including BUN concentration, there were no significant (p > 0.05) difference among treatments. As a result, although feed intake and weight gain with TDN/CP rate of 4:1 showed best results, considering the excess body fat accumulation possibilities or blood metabolism, it seems 4.5:1 ratio is most appropriate.

키워드

참고문헌

  1. Agricultural Research Council. 1980. The Nutrient Requirements of Ruminant Livestock. Commonwealth Agricultural Bureaux, Farnham Royal, UK. 121-166.
  2. Association of Official Agricultural Chemists, and William Horwitz. 2005. Official methods of analysis. Washington, DC: AOAC. 534.
  3. Barton, B.A., Rosario, H.A., Anderson, G.W., Grindle, B.P. and Carroll, D.J. 1996. Effects of dietary crude protein, breed, parity, and health status on the fertility of dairy cows. Journal of Dairy Science. 79. 2225-2236. https://doi.org/10.3168/jds.S0022-0302(96)76599-2
  4. Baek, K.S., Yoon, H.S., Lee, W.S., Ahn, H.S., Kim, W.H., Chae, H.S., Kim, N.Y. and Cho, I.C. 2015. Effect of age at the first pregnancy on calving status in holstein heifers. Annals of Animal Resources Sciences. 26. 13-20. https://doi.org/10.12718/AARS.2015.26.1.13
  5. Blome, R.M., Drackley, J.K., McKeith, F.K., Hutjens, M.F. and McCoy, G.C. 2003. Growth, nutrient utilization, and body composition of dairy calves fed milk replacers containing different amounts of protein. Journal of Animal Science. 81. 1641-1655. https://doi.org/10.2527/2003.8161641x
  6. Bodine, T.N. and Purvis, H.T. 2003. Effects of supplemental energy and/or degradable intake protein on performance, grazing behavior, intake, digestibility, and fecal and blood indices by beef steers grazed on dormant native tallgrass prairie. Journal of Animal Science. 81. 304-317. https://doi.org/10.2527/2003.811304x
  7. Broderick, G.A. 2003. Effects of varying dietary protein and energy levels on the production of lactating dairy cows. Journal of Dairy Science. 86. 1370-1381. https://doi.org/10.3168/jds.S0022-0302(03)73721-7
  8. Brown, E.G., Vandehaar, M.J., Daniels, K.M., Liesman, J.S., Chapin, L.T., Keisler, D.H. and Nielsen, M.S.W. 2005. Effect of increasing energy and protein intake on body growth and carcass composition of heifer calves. Journal of Dairy Science. 88. 585-594. https://doi.org/10.3168/jds.S0022-0302(05)72722-3
  9. Brugere-Picoux, J. and Brugere, H. 1980. Fat cow syndrome. Recueil de Medecine Veterinaire. 156. 195-200.
  10. Chalupa, W., O'Dell, G.D., Kutches, A.J. and Lavker, R. 1970. Supplemental corn silage or baled hay for correction of milk fat depressions produced by feeding pellets as the sole forage. Journal of Dairy Science. 53. 208-214. https://doi.org/10.3168/jds.S0022-0302(70)86181-1
  11. Choi, B.R. 2004. Nutritional supplementing ways on feeding and management of dairy cow. Journal of Veterinary Clinics. Proceedings of the congress Korean society of veterinary clinics. 5-22.
  12. Collier, R.J., Doelger, S.G., Head, H.H., Thatcher, W.W. and Wilcox, C.J. 1982. Effects of heat stress during pregnancy on maternal hormone concentrations, calf birth weight and postpartum milk yield of Holstein cows. Journal of Animal Science. 54. 309-319. https://doi.org/10.2527/jas1982.542309x
  13. Cozzi, G., Ravarotto, L., Gottardo, F., Stefani, A., Contiero, L., Moro, L., Brscic, M. and Dalvit, P. 2011. Short communcation: Reference values for blood parameters in Holstein dairy cows: Effects of parity, stage of lactation and season of production. Journal of Dairy Science. 94. 3895-3901. https://doi.org/10.3168/jds.2010-3687
  14. De La Sota, R.L., Lucy, M.C., Staples, C.R. and Thatcher, W.W. 1993. Effects of recombinant bovine somatotropin (sometribove) on ovarian function in lactating and nonlactating dairy cows. Journal of Dairy Science. 76. 1002-1013. https://doi.org/10.3168/jds.S0022-0302(93)77428-7
  15. Devant, M., Ferret, J., Gasa, S.C. and Casals, R. 2000. Effects of protein concentration and degradability on performance, ruminal fermentation, and nitrogen metabolism in rapidly growing heifers fed high-concentrate diets from 100 to 230 kg body weight. Journal of Animal Science. 78. 1667-1676. https://doi.org/10.2527/2000.7861667x
  16. Drackley, J.K. 2004. Feeding for Accelerated Growth in Dairy Calves. Proceedings of Minnesota Dairy Health Conference. USA. 59-73.
  17. Gabler, M.T. and Heinrichs, A.J. 2003. Dietary Protein to Metabolizable Energy Ratios on Feed Efficiency and Structural Growth of Prepubertal Holstein Heifers. Journal of Dairy Science. 86. 268-274. https://doi.org/10.3168/jds.S0022-0302(03)73605-4
  18. Gardner, R.W., Schuh, J.D. and Vargus, L.G. 1977. Accelerated growth and early breeding of Holstein heifers. Journal of Dairy Science. 60. 1941-1948. https://doi.org/10.3168/jds.S0022-0302(77)84126-X
  19. Gaynor, P.J., Waldo, D.R., Capuco, A.V., Erdman, R.A. and Douglass, L.W. 1995. Effect of prepubertal growth rate and diet on lipid metabolism in lactating cows. Journal of Dairy Science. 78. 1534-1543. https://doi.org/10.3168/jds.S0022-0302(95)76775-3
  20. Groff, E.B. and Wu, Z. 2005. Milk production and nitrogen excretion of dairy cows fed different amounts of protein and varying proportions of alfalfa and corn silage. Journal of Dairy Science. 88. 3619-3632. https://doi.org/10.3168/jds.S0022-0302(05)73047-2
  21. Hill, T.M., Bateman, H.G., Quigley, J.D., Aldrich, J.M., Schlotterbeck, R.L. and Heinrichs, A.J. 2013. Review: New information on the protein requirements and diet formulation for dairy calves and heifers since the Dairy NRC 2001. The Professional Animal Scientists. 29. 199-207. https://doi.org/10.15232/S1080-7446(15)30225-4
  22. Hoffman, P.C., Esser, N.M., Bauman, L.M., Denzine, S.L. and Engstrom, M. Chester-Jones, H. 2001. Short Communication: Effect of dietary protein on growth and nitrogen balance of Holstein heifers. Journal of Dairy Science. 84. 843-847. https://doi.org/10.3168/jds.S0022-0302(01)74542-0
  23. Huhtanen, P. and Hristov, A.N. 2009. A meta-analysis of the effects of dietary protein concentration and degradability on milk protein yield and milk N efficiency in dairy cows. Journal of Dairy Science. 92. 3222-3232. https://doi.org/10.3168/jds.2008-1352
  24. Jeong, K.S. 2013. Effects of heifer ratio on efficiency of dairy management. Korean Journal of Agricultural Management and Policy. 40. 1006-1016.
  25. Kauffman, A.J. and St-Pierre, N.R. 2001. The relationship of milk urea nitrogen to urine nitrogen excretion in Holstein and Jersey cows. Journal of Dairy Science. 84. 2284-2294. https://doi.org/10.3168/jds.S0022-0302(01)74675-9
  26. Ki, K.S., Lee, H.J., Kim, S.B., Lee, W.S. Lim, D.H., Lim, H.J., Park, S.J., Cho, W.M., Kim, H.S., Jin, Z.L., Lee, I.D., Kim, W.Y. and Jeo, J.M. 2010. Effects of different energy and protein level of TMR on milk production of dairy goats in early lactation. Journal of the Korean Society of Grassland and Forage Science. 30. 267-274. https://doi.org/10.5333/KGFS.2010.30.3.267
  27. Kohn, R.A., Dinneen, M.M. and Russek-Cohen, E. 2005. Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs, and rats. Journal of Animal Science. 83. 879-889. https://doi.org/10.2527/2005.834879x
  28. Lee, B.H., Nejad, J.G., Kim, H.S. and Sung, K.I. 2013. Effect of forage feeding level on the milk production characteristics of holstein lactating cows. Journal of the Korean Society of Grassland and Forage Science. 33. 45-51. https://doi.org/10.5333/KGFS.2013.33.1.45
  29. Marston, T.T. and Lusby, K.S. 1995. Effects of energy or protein supplements and stage of production on intake and digestibility of hay by beef cows. Journal of Animal Science. 73. 651-656. https://doi.org/10.2527/1995.733651x
  30. Moon, J.S., Joo, Y.S., Jang, G.C., Yoon, Y.D., Lee, B.K., Park, Y.H. and Son, C.H. 2002. Interpretation of protein-energy balance of feeding by milk urea nitrogen and milk protein contents in lactation holstein cow. Journal of Animal Science and Technology. 44. 573-584. https://doi.org/10.5187/JAST.2002.44.5.573
  31. National Livestock Research Institute. 2002. Standard tables of feed composition in Korea. Rural Development Administration.
  32. NRC (National Research Council). 2001. Nutrient requirements of dairy cattle. 7. 381.
  33. Park, S.B., Lim, D.H., Park, S.M., Kim, T.I., Choi, S.H., Kwon, E.G., Seo, J., Seo, S.W. and Ki, K.S. 2013. Effects of different energy and rumen undegradable protein levels on dairy cow's production performance at mid-lactation period. CNU Journal of Agricultural Science. 40. 333-338. https://doi.org/10.7744/cnujas.2013.40.4.333
  34. Reynal, S.M. and Broderick, G.A. 2005. Effect of dietary level of rumen-degraded protein on production and nitrogen metabolism in lactating dairy cows. Journal of Dairy Science. 88. 4045-4064. https://doi.org/10.3168/jds.S0022-0302(05)73090-3
  35. Rural Development Administration. 2007. Korean feeding standard for dairy cattle. National Institute of Animal Science.
  36. SAS. 2003. Statistical Analysis System, Version 9.1 USA.
  37. Son, C.H. 2012. Nutrition and reproduction of dairy cow. Journal of Veterinary Clinics. 29. 92-94.
  38. Steel, R.G.D. and Torrie, J.H. 1980. Principles and procedures of statistics: A biometric approach. Principles and procedures of statistics: a biometrical approach.