The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Inhibitory Effect of Chlorine Dioxide on Phenoloxidase Activation of the Indianmeal Moth, Plodia interpunctella

화랑곡나방(Plodia interpunctella)의 페놀옥시데이즈 활성화에 대한 이산화염소의 억제 효과

  • Kim, Minhyun (Department of Bioresource Sciences, Andong National University) ;
  • Kwon, Hyeok (College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Wook (College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 김민현 (안동대학교 생명자원과학과) ;
  • 권혁 (고려대학교 생명공학부) ;
  • 김욱 (고려대학교 생명공학부) ;
  • 김용균 (안동대학교 생명자원과학과)
  • Received : 2016.03.23
  • Accepted : 2016.05.24
  • Published : 2016.06.30
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Abstract

Phenoloxidase (PO) is an oxidizing enzyme and plays crucial roles in insect immunity and cuticle sclerotization. High oxidizing activity of chlorine dioxide gives effective control activities against microbes and insect pests. These allowed us to assess any inhibitory activity of chlorine dioxide against PO with respect to insect immunity. PO activities of the Indeanmeal moth, Plodia interpunctella, was detected in both hemocytes and plasma. Upon bacterial challenge, PO activity was significantly increased especially in plasma. However, the immune challenge coupled with chlorine dioxide treatment did not enhance PO activity. When different chlorine dioxide concentrations were incubated with activated PO by immune challenge, they did not inhibit the activated PO. These results indicate that chlorine dioxide suppresses PO activity by inhibiting PO activation.

페놀옥시데이즈(phenoloxidase: PO)는 산화효소로서 곤충의 면역 및 표피층 경화 반응에 중요한 생리적 기능을 담당한다. 이산화염소는 높은 산화력을 바탕으로 미생물 및 곤충에 이르기까지 다양한 살균 및 살충효과를 갖고 있다. 본 연구는 곤충의 면역계에 중요한 역할을 수행하는 PO를 대상으로 이산화염소의 억제작용을 분석하였다. 화랑곡나방의 PO 활성은 혈구 및 혈장 모두에서 검출되었다. 이 PO 활성은 세균 침입에 따라 특히 혈장에서 뚜렷한 증가를 나타냈다. 그러나 세균 처리와 함께 이산화염소가 동시에 처리되면 이러한 PO 활성 증가는 나타나지 않았다. 반면에 면역 처리로 활성화된 PO에 대해서 대해서는 억제효과를 주지 못했다. 본 연구는 이산화염소가 PO의 활성을 억제하며, 이러한 억제는 PO의 활성화 단계에서 일어나는 것으로 나타내고 있다.

Keywords

References

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