Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of a short-term in vitro exposure time on the production of in vitro produced piglets

  • Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kwak, Tae-Uk (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Joo-Young (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hyung, Nam-Woong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Eung-Woo (Animal Genetics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2016.05.12
  • 심사 : 2016.06.24
  • 발행 : 2016.07.01
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초록

Although piglets have been delivered by embryo transfer (ET) with in vitro produced (IVP) embryos and blastocysts, a success rate has still remained lower level. Unlike mouse, human, and bovine, it is difficult to a production of piglets by in vitro fertilization (IVF) because of an inappropriate in vitro culture (IVC) system in pig. Therefore, the present study was conducted to investigate whether minimized exposure time in IVC can improve the pregnancy and delivery rates of piglets. Immediately after IVM, the oocytes were denuded and co-incubated with freshly ejaculated boar semen for 3.5 to 4 hours at $38.5^{\circ}C$ under 5% $CO_2$ in air. To avoid long-term exposure to in vitro state, we emitted IVC step after IVF. After that the presumptive zygotes were transferred into both oviducts of the surrogate on the same day or 1 day after the onset of estrus. Pregnancy was diagnosed on day 28 after ET and then was checked regularly every month by ultrasound examination. The 3 out of 4 surrogates were determined as pregnant (75%) and a total of 5 piglets (2 females and 3 males) were delivered at $118.3{\pm}2.5$ days of pregnancy period. In conclusion, a short-term exposure time may be an important factor in the production of IVP-derived piglets. It can be apply to the in vitro production system of transgenic pig by IVF, cloning, and pronuclear microinjection methods.

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참고문헌

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