DOI QR코드

DOI QR Code

Complete Mitochondrial Genome of Anoplocephala magna Solidifying the Species

  • Guo, Aijiang (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
  • Received : 2016.01.03
  • Accepted : 2016.04.03
  • Published : 2016.06.30

Abstract

The 2 species of the genus Anoplocephala (Anoplocephalidae), A. perfoliata and A. magna, are among the most important equine cestode parasites. However, there is little information about their differences at the molecular level. The present study revealed that the mitochondrial (mt) genome of A. magna was 13,759 bp in size and 700 bp shorter than that of A. perfoliata. The 2 species includes 2 rRNA, 22 tRNA, and 12 protein-coding genes each. The size of each of the 36 genes was the same as that of A. perfoliata, except for cox1, rrnL, trnC, trnS2(UCN), trnG, trnH, trnQ, and trnP. In the full mitochondrial genome, the sequence similarity was 87.1%. The divergence in the nucleotide and amino acid sequences of individual protein-coding genes ranged from 11.1% to 16% and 6.8% to 16.4%, respectively. The 2 non-coding regions of the mt genome of A. magna were 199 bp and 271 bp in length, while the equivalent regions in A. perfoliata were 875 bp and 276 bp, respectively. The results of this study support the proposal that A. magna and A. perfoliata are separate species, consistent with previous morphological analyses.

Keywords

References

  1. Bohorquez GA, Luzon M, Martin-Hernandez R, Meana A. New multiplex PCR method for the simultaneous diagnosis of the three known species of equine tapeworm. Vet Parasitol 2015; 207: 56-63. https://doi.org/10.1016/j.vetpar.2014.11.002
  2. Jeon HK, Kim KH, Eom KS. Complete sequence of the mitochondrial genome of Taenia saginata: comparison with T. solium and T. asiatica. Parasitol Int 2007; 56: 243-246. https://doi.org/10.1016/j.parint.2007.04.001
  3. Jia WZ, Yan HB, Guo AJ, Zhu XQ, Wang YC, Shi WG, Chen HT, Zhan F, Zhang SH, Fu BQ, Littlewood DTK, Cai XP. Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 2010; 11: 447. https://doi.org/10.1186/1471-2164-11-447
  4. Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A. Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria. Int J Parasitol 2013; 43: 427-437. https://doi.org/10.1016/j.ijpara.2012.11.014
  5. Littlewood DT, Lockyer AE, Webster BL, Johnston DA, Le TH. The complete mitochondrial genomes of Schistosoma haematobium and Schistosoma spindale and the evolutionary history of mitochondrial genome changes among parasitic flatworms. Mol Phylogenet Evol 2006; 39: 452-467. https://doi.org/10.1016/j.ympev.2005.12.012
  6. von Nickisch-Rosenegk M, Brown WM, Boore JL. Complete sequence of the mitochondrial genome of the tapeworm Hymenolepis diminuta: gene arrangements indicate that Platyhelminths are Eutrochozoans. Mol Biol Evol 2001; 18: 721-730. https://doi.org/10.1093/oxfordjournals.molbev.a003854
  7. Guo A. The complete mitochondrial genome of Anoplocephala perfoliata, the first representative for the family Anoplocephalidae. Parasit Vectors 2015; 8: 549. https://doi.org/10.1186/s13071-015-1172-z
  8. Huang X, Madan A. CAP3: a DNA sequence assembly program. Genome Res 1999; 9: 868-877. https://doi.org/10.1101/gr.9.9.868
  9. Laslett D, Canback B. ARWEN: a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences. Bioinformatics 2008; 24: 172-175. https://doi.org/10.1093/bioinformatics/btm573
  10. Yamasaki H, Ohmae H, Kuramochi T. Complete mitochondrial genomes of Diplogonoporus balaenopterae and Diplogonoporus grandis (Cestoda: Diphyllobothriidae) and clarification of their taxonomic relationships. Parasitol Int 2012; 61: 260-266. https://doi.org/10.1016/j.parint.2011.10.007
  11. Okimoto R, Chamberlin HM, Macfarlane JL, Wolstenholme DR. Repeated sequence sets in mitochondrial DNA molecules of root knot nematodes (Meloidogyne): nucleotide sequences, genome location and potential for host-race identification. Nucleic Acids Res 1991; 19: 1619-1626. https://doi.org/10.1093/nar/19.7.1619
  12. Boore JL. Animal mitochondrial genomes. Nucleic Acids Res 1999; 27: 1767-1780. https://doi.org/10.1093/nar/27.8.1767
  13. Wolstenholme DR. Animal mitochondrial DNA: structure and evolution. Int Rev Cytol 1992; 141: 173-216.
  14. Monnerot M, Solignac M, Wolstenholme DR. Discrepancy in divergence of the mitochondrial and nuclear genomes of Drosophila teissieri and Drosophila yakuba. J Mol Evol 1990; 30: 500-508. https://doi.org/10.1007/BF02101105
  15. Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A. Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species. J Clin Microbiol 2009; 47: 168-174. https://doi.org/10.1128/JCM.01573-08
  16. McManus DP. Molecular discrimination of taeniid cestodes. Parasitol Int 2006; 55 (suppl): S31-S37. https://doi.org/10.1016/j.parint.2005.11.004
  17. Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K, Qiu D, Mamuti W, Craig PS, Ito A. DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR. J Clin Microbiol 2004; 42: 548-553. https://doi.org/10.1128/JCM.42.2.548-553.2004
  18. Gonzalez LM, Montero E, Morakote N, Puente S, Diaz De Tuesta JL, Serra T, Lopez-Velez R, McManus DP, Harrison LJ, Parkhouse RM, Garate T. Differential diagnosis of Taenia saginata and Taenia saginata asiatica taeniasis through PCR. Diagn Microbiol Infect Dis 2004; 49: 183-188. https://doi.org/10.1016/j.diagmicrobio.2004.03.013

Cited by

  1. Characterization of the complete mitochondrial genome of the cloacal tapeworm Cloacotaenia megalops (Cestoda: Hymenolepididae) vol.9, pp.1, 2016, https://doi.org/10.1186/s13071-016-1782-0
  2. Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily vol.10, pp.2, 2016, https://doi.org/10.3390/genes10020107