한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제45권7호
  • /
  • Pages.1035-1040
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  • 2016
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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장수지역 전통된장의 미생물 군집 및 바실러스 세레우스 길항 효과

Bacterial Community of Traditional Doenjang in Longevity Area and Antagonistic Effect against Bacillus cereus

  • 전두영 (전라남도 보건환경연구원 미생물과) ;
  • 윤기복 (전라남도 보건환경연구원 미생물과) ;
  • 윤연희 (전라남도 보건환경연구원 미생물과) ;
  • 양수인 (전라남도 보건환경연구원 미생물과) ;
  • 김중범 (순천대학교 식품공학과)
  • Jeon, Doo-Young (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Yoon, Gi-Bok (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Yoon, Yeon-Hee (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Yang, Soo-In (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Kim, Jung-Beom (Department of Food Science and Technology, Sunchon National University)
  • 투고 : 2016.03.31
  • 심사 : 2016.05.17
  • 발행 : 2016.07.31
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초록

본 연구는 우리나라 장수지역에서 제조된 전통된장을 수거하여 식중독세균 오염도와 미생물 군집 현황을 분석하고, 분리된 미생물 군집의 Bacillus cereus 길항 효과를 평가함으로써 전통된장의 미생물학적 안전성을 평가하고자 하였다. 전통된장의 미생물학적 안전성을 평가하기 위해 일반세균, 대장균군, 식중독세균을 실험한 결과 일반세균수는 $10^6{\sim}10^9CFU/g$의 분포를 나타내었고 대장균군은 불검출 되었다. 식중독세균 중 B. cereus는 전통된장 10개 중 7개에서 검출되었으나 기타 식중독균은 불검출 되었다. 실험 결과 장수지역 전통된장에 가장 문제가 되는 미생물은 B. cereus로 나타나 전통된장의 안전성 확보를 위해 B. cereus 제어가 중요한 것으로 판단되었다. 전통된장의 미생물 군집 분석결과 Bacillus subtilis가 155균주(47.4%)로 가장 높은 분포를 나타내었으며, B. licheniformis 68균주(20.8%), B. amyloliquefaciens 46균주(14.1%)로 높은 분포를 나타내는 등 총 327균주가 동정되었다. B. cereus에 대한 길항 효과를 평가한 결과 분리균주 327균주 중 20균주(6.1%)에서 9~12 mm 생육저지환이 관찰되었으며, B. subtilis가 12균주로 가장 많았고, B. amyloliquefaciens 5균주, B. licheniformis 3균주 순으로 분포하였다. 전통된장의 경우 B. cereus 오염도가 식품공장 제조 된장에 비해 높지만 B. cereus 길항효과를 나타내는 균주가 많은 것으로 판단되었다. 이러한 결과를 종합해 볼 때 전통된장에 오염된 B. cereus 위험성은 기존의 생각보다 낮을 것으로 판단되며, 향후 다수의 전통된장을 대상으로 한 B. cereus 길항 효과 실험이 필요한 것으로 판단되었다.

This study investigated the prevalence of foodborne pathogens and the bacterial community of traditional Doenjang collected from a longevity area in Korea as well as the antagonistic effect of traditional Doenjang isolates against Bacillus cereus to estimate the microbiological safety of traditional Doenjang. Aerobic bacteria showed $10^6{\sim}10^9CFU/g$, whereas coliform bacteria was not detected. Foodborne pathogens were not detected except B. cereus, which was detected in seven samples out of 10 Doenjang samples. A total of 327 isolates were identified from traditional Doenjang. The isolates consisted of Bacillus subtilis 155 (47.4%), Bacillus licheniformis 68 (20.8%), Bacillus amyloliquefaciens 46 (14.1%), and Bacillus pumilus 18 (5.5%). Antagonistic effect against B. cereus was detected in 20 (6.1%) of 327 isolates, which consisted of B. subtilis (12 strains), B. amyloliquefaciens (5 strains), and B. licheniformis (3 strains). The inhibitory zone for the antagonistic effect was 9.0~12.0 mm in diameter. Although a small amount of traditional Doenjang was tested in this study, these results indicated that the potential risk of B. cereus in traditional Doenjang is lower than generally presumed. It is necessary to monitor the antagonistic effect of traditional Doenjang isolates against B. cereus.

키워드

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