DOI QR코드

DOI QR Code

Copigments 처리에 의한 검정콩 안토시아닌 추출물의 저장 안정성

Storage Stability of Anthocyanin Extracted from Black Bean (Glycine max Merrill.) with Copigments Treatment

  • 지영미 (충북대학교 식품생명공학과) ;
  • 김민영 (충북대학교 식품생명공학과) ;
  • 이상훈 (충북대학교 식품생명공학과) ;
  • 장귀영 (충북대학교 식품생명공학과) ;
  • 윤나라 (충북대학교 식품생명공학과) ;
  • 김은희 (충북대학교 식품생명공학과) ;
  • 김경미 (농촌진흥청 국립농업과학원) ;
  • 이준수 (충북대학교 식품생명공학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Ji, Yeong Mi (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Min Young (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Sang Hoon (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Yoon, Nara (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Eun Hee (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Kyung Mi (Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Junsoo (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jeong, Heon Sang (Department of Food Science and Biotechnology, Chungbuk National University)
  • 투고 : 2016.03.03
  • 심사 : 2016.06.03
  • 발행 : 2016.07.31

초록

검정콩의 안토시아닌 색소의 안정성에 대한 유기산(succinic acid), 금속이온($Cu^{2+}$), ascorbic acid, 당류(sucrose) 및 pH의 영향을 $4^{\circ}C$$40^{\circ}C$에서 저장기간별로 살펴보았다. 모든 처리구에서 $4^{\circ}C$는 copigment 처리 여부에 관계없이 안정하였지만 $40^{\circ}C$에서는 copigment 처리구가 안정하였다. Succinic acid 처리는 $40^{\circ}C$에서 무처리에 비해 7~15% 안정성이 향상되었다. $Cu^{2+}$ 처리는 안토시아닌 색소와 반응하여 안정화에 효과가 있었으며, $4^{\circ}C$에서는 저장 8일차까지 100%로 유지되었다. Ascorbic acid 처리는 안정성을 저하했으며 $40^{\circ}C$에서는 무처리에 비해 64~72% 감소하였다. Sucrose 처리는 무처리에 비하여 효과가 작았으며 온도가 높을수록 안정성이 감소하였다. pH는 낮을수록 안정성이 증가하였으며 $4^{\circ}C$에 비하여 $40^{\circ}C$가 낮았다. 특히 pH 7에서는 급격히 감소하였다. 이러한 결과로부터 검정콩 안토시아닌 색소 저장 안정성 향상을 위해서는 온도, 유기산 및 pH 조절이 효과적이라고 판단된다.

Effects of copigments (succinic acid, $Cu^{2+}$, ascorbic acid, sucrose, and pH) on stability of anthocyanin extracted from black beans (Glycine max Merrill.) were investigated during storage for 8 days at 4 and $40^{\circ}C$. Succinic acid improved stability of anthocyanin by 7~15% compared to the non-treatment group at $40^{\circ}C$. $Cu^{2+}$ maintained stability of anthocyanin by about 100% for 8 days at $4^{\circ}C$. Ascorbic acid reduced stability of anthocyanin by 64~72% of non-treatment at $40^{\circ}C$. Sucrose treatment did not significantly affect stability compared to the control. The lower pH improved stability of anthocyanin. Stability at pH 1 was improved by 81~87% compared to that at pH 7. These results show that temperature, organic acid, and pH were effective in improving storage stability of anthocyanin from black beans.

키워드

참고문헌

  1. Chung KW, Joo YH, Lee DJ. 2004. Content and color difference of anthocyanin by different storage periods in seed coats of black soybean [Glycine max (L.) Merr.]. Korean J Intl Agri 16: 196-199.
  2. Francis FJ, Markakis PC. 1989. Food colorants: Anthocyanins. Crit Rev Food Sci Nutr 28: 273-314. https://doi.org/10.1080/10408398909527503
  3. Jackman RL, Yada RY, Tung MA, Speers RA. 1987. Anthocyanins as food colorants-A review. J Food Biochem 11: 201-247. https://doi.org/10.1111/j.1745-4514.1987.tb00123.x
  4. Brouillard R, Markakis P. 1982. Stability of anthocyanins in foods. In Anthocyanins as Food Colors. Markakis P, ed. Academic Press, New York, NY, USA. p 163-180.
  5. Reyes LF, Cisneros-Zevallos L. 2007. Degradation kinetics and colour of anthocyanins in aqueous extracts of purpleand red-flesh potatoes (Solanum tuberosum L.). Food Chem 100: 885-894. https://doi.org/10.1016/j.foodchem.2005.11.002
  6. Mazza G, Brouillard R. 1987. Recent developments in the stabilization of anthocyanins in food products. Food Chem 25: 207-225.
  7. Brouillard R, Dangles O. 1994. Anthocyanin molecular interactions: the first step in the formation of new pigments during wine aging?. Food Chem 51: 365-371. https://doi.org/10.1016/0308-8146(94)90187-2
  8. Hendry GAF, Houghton JD. 1996. Copigmentation. In Natural Food Colorants. Hendry GAF, Houghton JD, eds. Chapman and Hall, Glasgow, UK. p 267-270.
  9. Gauche C, Malagoli EDS, Bordignon Luiz MT. 2010. Effect of pH on the copigmentation of anthocyanins from Cabernet Sauvignon grape extracts with organic acids. Sci Agric 67: 41-46. https://doi.org/10.1590/S0103-90162010000100006
  10. Timberlake CF, Bridle P. 1982. Distribution of anthocyanins in food plants. In Anthocyanins as Food Colors. Markakis P, ed. Academic Press, New York, NY, USA. p 125-162.
  11. Rein M. 2005. Copigmentation reactions and color stability of berry anthocyanins. Academic Dissertation. University of Helsinki, Helsinki, Finland.
  12. Lee J, Durst RW, Wrolstad RE. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. J AOAC Int 88: 1269-1278.
  13. Lee LS, Rhim JW, Kim SJ, Chung BC. 1996. Study on the stability of anthocyanin pigment extracted from purple sweet potato. Korean J Food Sci Technol 28: 352-359.
  14. Kim HS, Ahn SY. 1978. Studies on the formation of anthocyanin metal complex. J Korean Agric Chem Soc 21: 22-30.
  15. Yoon JM, Cho MH, Hahn TR, Paik YS, Yoon HH. 1997. Physicochemical stability of anthocyanins from a Korean pigmented rice variety as natural food colorants. Korean J Food Sci Technol 29: 211-217.
  16. Wrolstad RE, Erlandson JA. 1973. Effect of metal ions on the color of strawberry puree. J Food Sci 38: 460-463. https://doi.org/10.1111/j.1365-2621.1973.tb01454.x
  17. Sondheimer E, Kertesz ZI. 1952. The kinetics of the oxidation of strawberry anthocyanin by hydrogen peroxide. J Food Sci 17: 288-298. https://doi.org/10.1111/j.1365-2621.1952.tb16766.x
  18. Markaris P, Livingston GE, Fellers CR. 1957. Quantitative aspects of strawberry of pigment degradation. J Food Sci 22: 117-130. https://doi.org/10.1111/j.1365-2621.1957.tb16991.x
  19. Shim KH, Kang KS, Choi JS, Seo KI, Moon JS. 1994. Isolation and stability of anthocyanin pigments in grape peels. J Korean Soc Food Nutr 23: 279-286.
  20. Hwang ES, Ki KN. 2013. Stability of the anthocyanin pigment extracted from aronia (Aronia melancocarpa). Korean J Food Sci Technol 45: 416-421. https://doi.org/10.9721/KJFST.2013.45.4.416
  21. Park SJ, Lee JH, Rhim JH, Kwon KS, Jang HG, Yu M. 1994. The change of anthocyanin and spreadmeter value of strawberry jam by heating and preservation. Korean J Food Sci Technol 26: 365-369.
  22. Park HJ, Jeon TW, Lee SH, Cho YS, Cho SM, Chang KS. 2004. Studies on characteristics and stability of anthocyanin pigment extracted from Korean purple-fleshed potatoes. J Korean Soc Food Sci Nutr 33: 1544-1551. https://doi.org/10.3746/jkfn.2004.33.9.1544
  23. Bassa LA, Francis FJ. 1987. Stability of anthocyanins from sweet potatoes in a model beverage. J Food Sci 52: 1753-1754. https://doi.org/10.1111/j.1365-2621.1987.tb05927.x

피인용 문헌

  1. Quality Characteristics of Inner Beauty Foods (Mook) Prepared with Mixture of Mulberry Leaf and Fruit Powder vol.16, pp.4, 2016, https://doi.org/10.20402/ajbc.2018.0238
  2. 고구마 끝순 및 괴근의 안토시아니딘 추출 조건 최적화 vol.52, pp.3, 2016, https://doi.org/10.9721/kjfst.2020.52.3.290