Acknowledgement
본 연구는 제이에스온(주)의 전임상 위탁 기술개발 지원(원내과제번호: PHX0154481) 및 보건산업 진흥원 의료기기 기술개발 사업의 지원(과제번호: H16C1104)을 받아 진행되었음.
Clinical Pain
- Volume 15 Issue 2
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- Pages.92-96
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- 2016
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- 1598-5490(pISSN)
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- 2765-5156(eISSN)
Effect of Transcutaneous High Frequency Wave on the Change of Tissue Temperature and Histology in Sprague-Dawley Rat
백서에서 경피적 고주파 자극에 따른 온도 변화 및 조직 변화
- Kim, Kyung Ah (Department of Physical and Rehabilitation Medicine, Samsung Medical Center) ;
- Moon, Chang Won (Department of Physical and Rehabilitation Medicine, Samsung Medical Center) ;
- Song, Da-Hyun (Department of Physical and Rehabilitation Medicine, Samsung Medical Center) ;
- Kim, Sang Jun (Department of Physical and Rehabilitation Medicine, Samsung Medical Center)
- Received : 2016.05.20
- Accepted : 2016.11.24
- Published : 2016.12.31
Abstract
Objective: High frequency wave has been used in cancer treatment and cosmetic area but not in musculoskeletal pain yet. The purpose of this study is to evaluate temperature distribution according to depth and confirm safety of high frequency wave through animal study. Method: High frequency wave was applied to the posterior limb of 9 Sprague-Dawley rats for 20 minutes (experimental group) and no wave was used in the same number of rats for control group. Tissue temperature was measured from skin surface to 1 cm depth (surface, 1 mm, 5 mm, and 1 cm) for 5 seconds. Results: In the experimental group, temperature was elevated 3.2℃ at skin surface, 2.87℃ at 1 mm, 2.25℃ in 5 mm, and 1.74℃ in 1 cm depth. These were significantly different from those in the control group (p<0.001). There was no bulla or redness in the skin after high frequency wave stimulation and neither change of myocytes nor collagen degeneration was found in the tissue histology. There was no apoptosis in the skin surface and muscle layer in TUNEL assay. Conclusion: High frequency wave elevated tissue temperature from the skin to muscle layer without both histologic change and apoptosis.