Particle and aerosol research (한국입자에어로졸학회지)

Korean Association for Particle and Aerosol Research (한국입자에어로졸학회)
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Characteristics of flow field of nose-only exposure chamber for inhalation toxicity evaluation

흡입독성평가를 위한 비부노출 챔버의 유동흐름 특성

  • Noh, Hakjae (Greensolus Co., Ltd.) ;
  • Bong, Choonkeun (Greensolus Co., Ltd.) ;
  • Bong, Hakyung (Greensolus Co., Ltd.) ;
  • Kim, Yonggu (Greensolus Co., Ltd.) ;
  • Cho, Myunghaing (Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Kim, Sanghwa (Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Kim, Daesung (Global Frontier Center for Multiscale Energy Systems, Seoul National University)
  • 노학재 ((주)그린솔루스) ;
  • 봉춘근 ((주)그린솔루스) ;
  • 봉하경 ((주)그린솔루스) ;
  • 김용구 ((주)그린솔루스) ;
  • 조명행 (서울대학교 수의과대학 독성학연구실) ;
  • 김상화 (서울대학교 수의과대학 독성학연구실) ;
  • 김대성 (서울대학교 멀티스케일 에너지 시스템 연구단)
  • 투고 : 2015.10.30
  • 심사 : 2016.02.12
  • 발행 : 2016.03.31
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초록

In this work, we evaluated the characteristics of flow field and uniformity of the nose-only exposure chambers for the inhalation toxicity test. Computational fluid dynamics (CFD) modeling was carried out to demonstrate uniformity of the nose-only exposure chambers. Because it is very important in the inhalation toxicity experiments that test materials are distributed uniformly to each holder of the chamber. The test was done with these 3 types of chamber with different form to develop inhalation toxicity evaluation system, easy-to-operate system among exposure chamber used for evaluating inhalation toxicity of environmental chemical mixtures. Through CFD interpretation, nose-only exposure chamber was made with the selection of the optimal conditions. For its evaluation, one type of fragrance was selected and measured particle size distribution of each port. The gene becoming luminous to green fluorescence was combined with GPT-SPE, a type of tGFP vector, to be inhaled to the mouse. Based on this, luminous intensity was checked. As a result, total particle number concentration of each port had average value of $3.17{\times}10^6{\sharp}/cm^3$ and range of the highest and lowest concentration value was approximately ${\pm}4.8%$. Autopsy of lung tissues of mouse showed that it had clearly better delivery of gene compared to the control group.

키워드

참고문헌

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