Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A 24-Weeks Toxicity Study of Eryngium foetidum Linn. Leaves in Mice

  • Received : 2015.06.04
  • Accepted : 2016.03.23
  • Published : 2016.07.15
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Abstract

Eryngium foetidum Linn. leaves (EF) are widely used in Thailand and many countries throughout Asia as a culinary seasoning and a traditional medicine. However, adverse effect of high dose consumption in long duration has not been evaluated. The aim of this study was to investigate chronic toxicity of EF in mice. Thirty-two ICR male mice were divided into 4 groups of 8 mice each. The mice were fed AIN-76 rodent diet, or AIN-76 rodent diet supplemented with ground freeze-dried EF at 0.8%, 1.6% and 3.2% that is equivalent to approximately 35, 73 and 155 times that of human consumption, respectively, at 97.5 percentile for a period of 24 weeks. At the end of experiment, the mice were euthanized and blood samples were collected for hematological and biochemical evaluations. Necropsy was performed while visceral organs such as lung, liver, kidneys, spleen etc. were collected, weighed and histopathologically examined. Blood urea nitrogen (BUN) results of mice in 1.6% and 3.2% EF diet groups were significantly higher than the BUN of control group. No significant difference was noted in other biochemical and hematological properties between the treatment groups and control; all results were within normal range. Histopathology of almost all visceral organs showed no significant changes. However, tubulonephrosis and chronic interstitial nephritis were observed in the groups treated with 1.6% and 3.2% EF diet. Body weight was reduced significantly at week 12 to week 20 when compared to the control group while relative kidney weights were significantly increased. In conclusion, the consumption of EF in diet at high doses illustrated the adverse effect on some biochemical parameters and histopathology in mice. Our findings suggested that EF daily consumption for 24 weeks, at higher doses than the 0.8% EF diet (35 times of human consumption), might cause adverse effect on kidney function in mice.

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References

  1. Adams, C.D. (1972) Flowering plants of Jamaica. University of the West Indies, Mona.
  2. Ekpong, B. Seed development and umble order contribution on eryngo (Eryngium foetidum L.) seed yield and quality [dissertation]. [Bangkok]: Kasetsart University; 2005.
  3. Paul, J.H.A., Seaforth, C.E. and Tikasingh, T. (2011) Eryngium foetidum L.: A review. Fitoterapia, 82, 302-308. https://doi.org/10.1016/j.fitote.2010.11.010
  4. Cardozo, E., Rubio, M., Rojas, L.B. and Alfredo, U. (2004) Composition of the essential oil from the leaves of Eryngium foetidum L. from the Venezuelan Andes. J. Essent. Oil Res., 16, 33-34. https://doi.org/10.1080/10412905.2004.9698645
  5. Mekhora, C., Muangnoi, C., Chingsuwanrote, P., Dawilai, S., Svasti, S., Chasri, K. and Tuntipopipat, S. (2012) Eryngium foetidum suppresses inflammatory mediators produced by macrophages. Asian Pac. J. Cancer Prev., 13, 653-664. https://doi.org/10.7314/APJCP.2012.13.2.653
  6. Chanwitheesuk, A., Teerawutgulrag, A. and Rakariyatham, N. (2005) Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem., 92, 491-497. https://doi.org/10.1016/j.foodchem.2004.07.035
  7. Garcia, M.D., Saenz, M.T., Gomez, M.A. and Fernandez, M.A. (1999) Topical anti-inflammatory activity of phytosterols isolated from Eryngium foetidum on chronic and acute inflammation models. Phytother. Res., 13, 78-80. https://doi.org/10.1002/(SICI)1099-1573(199902)13:1<78::AID-PTR384>3.0.CO;2-F
  8. Bonikowski, R., Kula, J., Bujacz, A., Wajs-Bonikowska, A., Zaklos-Szyda, M. and Wysocki, S. (2012) Hydroindenederived chiral synthons from carotol and their cytotoxicity. Tetrahedron Asymmetry, 23, 1038-1045. https://doi.org/10.1016/j.tetasy.2012.07.005
  9. Kawaree, R. Chemical component variation, cytotoxicity, antioxidant and stability of volatile oils from Thai medicinal plants [dissertation]. [Chiang Mai]: Chiang Mai University; 2007.
  10. Promkum, C., Butryee, C., Tuntipopipat, S. and Kupradinun, P. (2012) Anticlastogenic effect of Eryngium foetidum L. assessed by erythrocyte micronucleus assay. Asian Pac. J. Cancer Prev., 13, 3343-3347. https://doi.org/10.7314/APJCP.2012.13.7.3343
  11. Sartorelli, D.S., Franco, L.J. and Cardoso, M.A. (2008) High intake of fruits and vegetables predicts weight loss in Brazilian overweight adults. Nutr. Res., 28, 233-238. https://doi.org/10.1016/j.nutres.2008.02.004
  12. Slavin, J.L. (2005) Dietary fiber and body weight. Nutrition, 21, 411-418. https://doi.org/10.1016/j.nut.2004.08.018
  13. Chivapat, S., Sincharoenpokai, P., Suppajariyawat, P., Rungsipipat, A., Phattarapornchaiwat, S. and Chantarateptawan, V. (2012) Safety evaluations of ethanolic extract of moringa oleifera lam seed in experimental animals. Thai J. Vet. Med., 42, 343-352.
  14. Ratnayake, W.M., L'Abbe, M.R., Mueller, R., Hayward, S., Plouffe, L., Hollywood, R. and Trick, K. (2000) Vegetable oils high in phytosterols make erythrocytes less deformable and shorten the life span of stroke-prone spontaneously hypertensive rats. J. Nutr., 130, 1166-1178. https://doi.org/10.1093/jn/130.5.1166
  15. Peckham, J.C. (2002) Animal Histopathology in CRC Handbook of Toxicology (Derelanko, M.J. and Hollinger, M.A.). CRC Press, New York, pp. 485-514.
  16. Marlett, J.A., McBurney, M.I. and Slavin, J.L. (2002) Position of the American Dietetic Association: health implications of dietary fiber. J. Am. Diet. Assoc., 102, 993-1000. https://doi.org/10.1016/S0002-8223(02)90228-2
  17. Porte, R.S. and Kaplan, J.L. (2011) The Merck Manual of Diagnosis and Therapy (19th edition) Planet Friendly Publishing, USA.
  18. Dreher, M.L. (2001) Dietary Fiber Overview in Handbook of Dietary Fiber (Cho, S.S. Ed.). CRC Press, India.