Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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Effects of the Radiolysis Products of Sennoside A on HepG2 and PC-3 Cells

세노사이드 A 방사선분해 산물의 HepG2 및 PC-3 세포에 대한 영향

  • Kim, Dong Ho (Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jo, Min Ho (Research Division for Biotechnology, Korea Atomic Energy Research Institute)
  • 김동호 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 조민호 (한국원자력연구원 첨단방사선연구소 생명공학연구부)
  • Received : 2016.05.06
  • Accepted : 2016.06.12
  • Published : 2016.06.30

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Abstract

Radiolysis of sennoside A was carried out by gamma irradiation and the anti-cancer activities of the radiolysis product were evaluated. An aqueous solution of sennoside A was exposed to 0.5~3 kGy of gamma irradiation and the radiolysis products were analyzed by HPLC. A fraction of radiolysis product (RLF) of sennoside A was isolated and the RLF was presumed as a rhein-8-β-D-glucoside. The anticancer effect of the RLF was compared with the sennoside and rhein using a in vitro assay system of human prostate cancer cells (PC-3) and human hepatoma HepG2 cells. The cell viability of PC-3 and HepG2 cell was significantly decreased to 12.4±1.2% and 32.4±2.1%, respectively, by the treatment of 0.6 μM of RLF. The sennoside A (range from 0 to 25 μM) had no cytotoxic effect on PC-3 and HepG2 cells, while the rhein had the effect on HepG2 cells with a LD50 at 80 μM.

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Acknowledgement

본 연구는 한국원자력연구원 기본연구사업 연구비 지원에 의하여 수행되었으며 이에 감사드립니다.