식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제46권2호
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  • Pages.163-174
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  • 2016
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
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Identification of Cambodian Gnetum (Gnetaceae, Gnetales) species by DNA barcoding

  • Kim, Joo Hwan (Department of Biological Science, Daegu University) ;
  • Won, Hyosig (Department of Biological Science, Daegu University)
  • 투고 : 2016.06.08
  • 심사 : 2016.06.13
  • 발행 : 2016.06.30
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초록

Gnetum (Gnetaceae, Gnetales) is a gymnosperm genus with ca. 35 species distributed in tropical forests around the world. Due to its dioecious habit and lack of diagnostic characters from vegetative tissue, the identification of Gnetum species is not easy without seeds or reproductive structures. To identify and verify their phylogenetic positions, we applied DNA barcoding to Cambodian Gnetum collections gathered between 2010 and 2015, with previously designed cp matK gene primers. We newly sequenced partial matK sequences from 72 Gnetum collections, 43 out of 72 from Cambodia, and analyzed 115 Gnetum accessions using the neighbor-joining method. The resulting neighbor-joining tree categorized Cambodian Gnetum samples into three clades of species: G. macrostachyum, G. montanum, and G. aff. gracilipes. The recognition of G. aff. gracilipes in Cambodia is reported here for the first time. Taxonomic information for the three recognized Cambodian Gnetum species is provided and the benefits of the taxonomic reevaluation assisted by DNA barcoding are emphasized in this work.

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참고문헌

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피인용 문헌

  1. Protognetaceae: A new gnetoid macrofossil family from the Jurassic of northeastern China vol.28, 2017, https://doi.org/10.1016/j.ppees.2017.08.001
  2. A Synopsis of the Genus Gnetum (Gnetaceae) in India vol.57, pp.1, 2016, https://doi.org/10.5735/085.057.0118