한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제39권2호
  • /
  • Pages.87-92
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  • 2016
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
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국내 분리 돼지 부종병 대장균의 병원성 유전자 및 시가독소 생성 검증

Evaluation of the virulence genes and Shiga toxin-producing abilities of Escherichia coli field isolates causing edema disease in pigs

  • Seo, Byoung-Joo (College of Veterinary Medicine, Chonbuk National University) ;
  • Jeong, Chang-Gi (College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, A-Rum (College of Veterinary Medicine, Chonbuk National University) ;
  • Cho, Ho-Seong (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Won-Il (College of Veterinary Medicine, Chonbuk National University)
  • 투고 : 2016.02.13
  • 심사 : 2016.05.29
  • 발행 : 2016.06.30
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초록

Porcine edema disease (ED) is a communicable disease of pigs caused by infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) which expresses F18 fimbriae and/or Stx type 2e (Stx2e). While STEC causes a severe illness including hemorrhagic colitis and hemolytic-uremic syndrome in humans, it induces damage to the vascular endothelium, which results in edema, hemorrhage, and microthrombosis, leading in high mortality in pigs. In the present study, we cultured Stx2e-producing E. coli field isolates from conventional pig farms that experienced sudden deaths previously with symptoms similar to porcine edema disease, which were further investigated with Shiga toxin profiles. A total of 43 strains were identified from the collected samples by F18 or Stx2e specific PCR. Based on the PCR, 42 isolates out of 43 isolates were proved to carry one of F18 or Stx2e genes and 14 isolates to carry both F18 and Stx2e genes. All of the 30 isolates that harbored Stx2e gene induced the cytopathic effect (CPE) in vero cells and especially, the isolate 150229 produced the highest level of Shiga toxin. Therefore, we identified the virulence genes (F18 and Stx2e) and demonstrated Shiga toxin-producing abilities from porcine edema disease causing E. coli filed isolates. These results suggested that one of the isolates could be a vaccine antigen candidate against STEC through further investigating to elicit an immune response.

키워드

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피인용 문헌

  1. Antimicrobial resistance of Stx2e positive Escherichia coli before and after ban on antibiotic growth promoters vol.18, pp.3, 2016, https://doi.org/10.12729/jbtr.2017.18.3.084