Advances in nano research

Techno-Press (테크노프레스)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of antibacterial activity and cytotoxic effects of green AgNPs against Breast Cancer Cells (MCF 7)

  • Received : 2016.01.06
  • Accepted : 2016.05.24
  • Published : 2016.06.25
⟨ Previous Next ⟩

Abstract

The present work reports a facile, rapid and an eco-friendly method for the synthesis of silver nanoparticles using Luffa acutangula (L. acutangula) leaves extract and their antibacterial and cytotoxic effects. The synthesized silver nanoparticles (AgNPs) were characterized by UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction analysis (XRD). Additionally the topography, morphology and the elemental composition of the particles were determined by Scanning Electron Microscopy (SEM) and Energy dispersive spectrophotometric (EDS) technique and the measured particle sizes from SEM micrographs are in the range of 12.5 to 24.5nm. The in-vitro antimicrobial activity of the synthesized nanoparticles was high against gram positive Staphylococcus aureus and moderate against gram negative Escherichia coli and Pseudomonas aeruginosa strains. Further, the cytotoxic effects of synthesized AgNPs were evaluated against Human Breast Cancer (MCF 7) cell line.

Keywords

References

  1. Aneja, K.R. (2003), Experiments in microbiology plant pathology tissue culture and mushroom production technology, 3rd Edition, New Age International Publisher, India
  2. Arunachalam, K.D., Annamalai, S.K. and Hari, S. (2013), "One-step green synthesis and characterization of leaf extract-mediated biocompatible silver and gold nanoparticles from Memecylon umbellatum", Int. J. Nanomed., 8, 1307. https://doi.org/10.2217/nnm.12.176
  3. Ashok Kumar, D., Palanichamy, V. and Mohana Roopan, S. (2014), "Green synthesis of silver nanoparticles using Alter nanthera dentata leaf extract at room temperature and their antimicrobial activity", Spectrochim Acta Part A: Mol Biomolspe., 127, 168-171. https://doi.org/10.1016/j.saa.2014.02.058
  4. Aswathy Aromal, S. and Philip, D. (2012), "Green synthesis of gold nanoparticles using Trigonella foenum-graecum and its size-dependent catalytic activity", Mol. Biomol. Spectrosc, 97, 1-5. https://doi.org/10.1016/j.saa.2012.05.083
  5. Awal, M.A., Ahsan, S.M.E., Haque, Q.H. and Asghor, M.D. (2010), "In-vitro activity of leaf and root extract of Cassia fistula", Med. Col. J., 3, 10-13.
  6. Awwad, M.A., Nida, M.S. and Amany, O.A. (2013), "Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity", Int. J. Indus. Chem., 4, 29. https://doi.org/10.1186/2228-5547-4-29
  7. Bijanzadeh, V.M., Vakili, R. and Khordad, R. (2012), "a study of the surface Plasmon absorption band for nanoparticles", Int. J. Phys. Sci., 7(12), 1943-1948.
  8. Garima, S., Riju, B., Kunal, K., Ashish, R.S. and Rajendra, P.S. (2011), "Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity", J. Nanopart Res., 13, 2981-2988. https://doi.org/10.1007/s11051-010-0193-y
  9. Geetha, A.R., George, E., Srinivasan, A. and Shaik, J. (2013), "Optimization of green synthesis of silver nanoparticles from leaf extracts of Pimentadioica (Allspice)", Scientif. World J., ID 362890.
  10. Gopinath, K., Gowri, S. and Arumugam, A. (2013), "Phytosynthesis of silver nanoparticles using Pterocarpus santalinus leaf extract and their antibacterial properties", J. Nanost.Chem., 3, 68. https://doi.org/10.1186/2193-8865-3-68
  11. Govender, R., Phulukdaree, A., Gengan, R.M., Anand, K. and Chuturgoon, A.A. (2013), "Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line", J. Nanobiotechnol, 11, 5 https://doi.org/10.1186/1477-3155-11-5
  12. Gurunathan, S., Kalishwaralal, K., Vaidyanathan, R., Deepak, V., Pandian, S.R.K. and Muniyandi, J. (2009), "Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli", J. Colloid. Surf. B, 74(1), 328-335. https://doi.org/10.1016/j.colsurfb.2009.07.048
  13. Husseiny, M.I., Abd El-Aziz, M., Badr, Y. and Mahmoud, M.A. (2007), "Biosynthesis of gold nanoparticles using Pseudomonas Aeruginosa", Spectrochim Acta Part A, 67(3-4), 1003-1006. https://doi.org/10.1016/j.saa.2006.09.028
  14. Indhumathy, J., Gurupavithra, S., Ravishankar, K. and Jayachitra, A. (2014), "Green synthesis of silver nanoparticles using Cassia fistula leaf extract and its applications", Mintage J. Pharm Medsci., 320-25.
  15. Jeyaraj, M., Sathishkumar, G., Sivanandhan, G., Mubarak Ali, D., Rajesh, M., Arun, R., Kapildev, G., Manickavasagam, M., Thajuddin, N., Premkumar, K. and Ganapathi, A. (2013), "Biogenic silver nanoparticles for cancer treatment: an experimental report", Coll. Surf B: Biointerf., 106, 86-92 https://doi.org/10.1016/j.colsurfb.2013.01.027
  16. Krasteva, N., Besnard, I., Guse, B., Bauer, R.E., Mullen, K. and Yasuda, A. (2002), "Self Assembled gold nanoparticle/ dendrimer composite films for vapour sensing applications", Nano Lett., 2, 551-555. https://doi.org/10.1021/nl020242s
  17. Kuldeep, G., Shabiha, N.H., Devabrata, S., Nima. D.N. and Manabendra, M. (2014), "One step green synthesis and anti-microbial and anti-biofilm properties of Psidiumguajava L. leaf extract-mediated silver nanoparticles", Mater. Lett., 125, 67-70. https://doi.org/10.1016/j.matlet.2014.03.134
  18. Moddab, S., Ahari, H., Shahbazzadeh, D., Motallebi, A.A., Anvar, A.A., Rahman-Nya, J. and Shokrgozar, M.R. (2011), "Toxicity study of nanosilver (Nanocid) on osteoblast cancer cell line", Int. Nano Lett, 1, 11-16.
  19. Moideen, SR. and Prabha, LA. (2014), "Green synthesis of silver nanoparticles using Luffa Acutungula Roxb.Var.Amara.Lin.and its antimicrobial activity", Int. J. Pharm Bio Sci, 5(4), 1051-1061.
  20. Muthu Irulappan, S., Bharath Manikanth, S., Kalishwaralal, K. and Gurunathan, S. (2010), "Antitumor activity of silver nanoparticles in Dalton's Lymphoma ascites tumor model", Int. J. Nanomed., 5, 753-762.
  21. Nagajyothi, P.C., Lee, K.D. and Sreekanth, T.V.M. (2013), "Plants as green source towards synthesis of nanoparticles", J. Optoelectron Adv. Mater., 15, 269
  22. Nagajyothi, P.C., Sreekanth, T.V.M., Prasad, T.N.V.K.V. and Lee, K.D. (2012), "Green synthesis of silver and gold nanoparticles using Lonicera Japonica flower extract", Adv. Sci. Lett., 5, 124 https://doi.org/10.1166/asl.2012.1948
  23. Panneer selvam, C., Ponarul selvam, S. and Murugan, K. (2011), "Potential anti-plasmodial activity of synthesized silver nanoparticles using Andrographis paniculatnees (Acanhaceae)", J. of Ecobiotechnolog, 3, 24-28.
  24. Phillip, D. (2010), "Gnidia glauca flower extract mediated synthesis of gold nanoparticles and evaluation of its chemo catalytic potential", Mol. Biomol. Spectrosc., 77, 807. https://doi.org/10.1016/j.saa.2010.08.008
  25. Prabha, S., Supraja, N., Garud, M. and Prasad, T.N.V.K.V. (2014), "Synthesis, characterization and antimicrobial activity of Alstonia scholaris bark-extract-mediated silver nanoparticles", J. Nanostruct. Chem., 4(4), 161-170. https://doi.org/10.1007/s40097-014-0132-z
  26. Prabhu, D., Arulvasu, C., Babu, G., Manikandan, R. and Srinivasan, P. (2013), "Biologically synthesized green silver nanoparticles from leaf extract of Vitex Negundo L. induce growth-inhibitory effect on human colon cancer cell line - HCT15", Proc. Biochem., 48, 317-324 https://doi.org/10.1016/j.procbio.2012.12.013
  27. Praksah, P., Gnana prakasam, P., Emmanuel, R., Arokiyaraj, S. and Saravanan, M. (2013), "Green Synthesis of silver nanoparticles from leaf extract of Mimusopselengi Linn. For enhanced antibacterial activity against multi drug resistance clinical isolates", Coll. Surf B: Biointerf., 108, 255-259. https://doi.org/10.1016/j.colsurfb.2013.03.017
  28. Qian, S., Xing, C., Jiangwei, L., Min, Z., Zuliang, C. and Chang-Ping, Y. (2014), "Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity", Coll. Surfaces A. Physicochem. Eng. Aspects, 444, 226-231. https://doi.org/10.1016/j.colsurfa.2013.12.065
  29. Satyavani, K., Gurudeeban, S., Ramanathan, T. and Balasubramanian, T. (2011), "Biomedical potential of silver nanoparticles synthesized from callicells of Citrullus colocynthis (L.) Schrad", J. Nanobiotechnol., 9, 43 https://doi.org/10.1186/1477-3155-9-43
  30. Selvaraj, A., Mariadhas, V.A., Savariar, V., Nyayirukannaian, U., Seong Ho Choi. and Kyoung Hoon Kim. (2014), "Rapid green synthesis of silver nanoparticles from chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study", Int. J. Nanomed., 9, 379-388.
  31. Subba Rao, Y., Kotakadi, V.S., Prasad, T.N.V.K.V., Reddy, A.V. and Sai Gopal, D.V.R. (2013), "Green synthesis and spectral characterization of silver nanoparticles from Lakshmi tulsi (Ocimum sanctum) leaf extract", Spectrochim Acta Part A: Mol Biomolspec., 103, 156-159. https://doi.org/10.1016/j.saa.2012.11.028
  32. Sulaiman, G.M., Mohammed, W.H., Marzoog, T.R., Amir Al-Amiery, A.A., Khadhum, A.A.H. and Mohamad, A.B. (2013), "Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract", Asian Pac. J. Trop Biomed., 3(1), 58-63. https://doi.org/10.1016/S2221-1691(13)60024-6
  33. Sunil, P., Goldie, O., Mewada, A. and Sharon, M. (2012), "Green synthesis of highly stable gold nanoparticles using Momordica charantia as nano fabricato", Arch. Appl. Sci. Res., 4, 1135.
  34. Supraja, N., Prasad, T.N.V.K.V., Giridhara Krishna, T. and David, E. (2015), "Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles", Appl. Nanosci., 6(4), 581-590
  35. Tetty, C.O., Nagajyothi, P.C., Lee, S.E., Ocloo, A., Minh, T.N., Sreekanth, T.V.M. and Lee, K.D. (2012), "Anti-melanoma, tyrosinase inhibitory and anti-microbial activities of gold nanoparticles synthesized from aqueous leaf extracts of Teraxacum officinale", Int. J. Cosmet. Sci., 34, 150 https://doi.org/10.1111/j.1468-2494.2011.00694.x
  36. Tupe, S.B., Patil, P.D., Thoke, R.B. and Aparadh, V.T. (2013), "Phytochemical screening in some cucurbitaceae members", Int. Res. J. Pharm. App. Sci., 3(1), 49-51.

Cited by

  1. Phytogenic silver nanoparticles (Alstonia scholaris) incorporated with epoxy coating on PVC materials and their biofilm degradation studies vol.4, pp.4, 2016, https://doi.org/10.12989/anr.2016.4.4.281
  2. Synthesis, characterization and dose dependent antimicrobial and anti-cancerous activity of phycogenic silver nanoparticles against human hepatic carcinoma (HepG2) cell line vol.3, pp.4, 2016, https://doi.org/10.3934/bioeng.2016.4.425