한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제44권2호
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  • Pages.180-184
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  • 2016
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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유입하수 첨가 배지를 이용한 세균 신분류군의 분리

Isolation of Novel Taxa Using Complex Media with Influent Sewage Water

  • 이시원 (국립환경과학원 상하수도연구과) ;
  • 박수정 (국립환경과학원 상하수도연구과) ;
  • 김창수 (국립환경과학원 상하수도연구과) ;
  • 조양석 (국립환경과학원 상하수도연구과) ;
  • 정현미 (국립환경과학원 상하수도연구과) ;
  • 박상정 (국립환경과학원 상하수도연구과)
  • Lee, Siwon (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Su Jeong (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Kim, Changsoo (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Cho, Yangsoek (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Chung, Hyen-Mi (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Sangjung (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
  • 투고 : 2016.02.16
  • 심사 : 2016.03.24
  • 발행 : 2016.06.28
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초록

본 연구에서는 신분류군 탐색을 위하여 유입하수 첨가 배지를 제작하였다. 유입하수 첨가배지에서는 일반 배지에서 분리 또는 동정되지 않은 13속 세균들을 배양하였으며, 특히 25에 비해 37℃에서 다양한 균주들을 배양할 수 있었다. 또한 유입하수 첨가 배지에서 총 12개의 분리 균주들이 97% 이하의 유사성을 나타내는 것으로 분석되었으며, 이들은 Tessaracoccus, Paracoccus 및 Candidimonas속(또는 Paralcaligenes속)과 유연관계가 있었다.

In this study, we evaluated complex media with influent sewage water (ISW) to isolate novel taxa of bacteria. It was possible to cultivate 13 genera using the complex media with ISW. Additionally, more diverse genera were identified at 37℃ than at 25℃, using the complex media with ISW. Total 12 strains of 179 bacterial isolates were shared less than 97% 16S rRNA gene sequence similarities with any known species. These isolates could be assigned to genera Tessaracoccus, Paracoccus, or Candidimonas (or Paralcaligenes).

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참고문헌

  1. Chun J, Bae KS. 2000. Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences. Antonie van Leeuwenhoek 78: 123−127. https://doi.org/10.1023/A:1026555830014
  2. Chung WY. 2003. A study on the coliforms removal methods at wastewater treatment plants. Master Thesis. Seoul, Konkuk University.
  3. Hall TA. 1999. Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp. Ser. 41: 95−98.
  4. Hong JH, Chung JD. 2005. Effect of broth of purple photosynthetic bacteria on garbage. J. Korea Soc. Waste Manag. 22: 113−119.
  5. Jiang X, Ma M, Li J, Lu A, Zhong Z. 2008. Bacterial diversity of active sludge in wastewater treatment plant, Earth Sci. Fron. 15: 163−168. https://doi.org/10.1016/S1872-5791(09)60015-4
  6. Kim DH, Lee SH, Cho JC. 2008. Evaluation of various oligotrophic media for cultivation of previously uncultured soil bacteria. Korean J. Microbiol. 44: 352−357.
  7. Kim MS, Baek JS. 2005. Microbial hydrogen production: Dark anaerobic fermentation and photo-biological process. Korean J. Biotechnol. Bioeng. 20: 393−400.
  8. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, et al. 2012. Introducing EzTaxon: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62: 716–721. https://doi.org/10.1099/ijs.0.038075-0
  9. Lee S. 2010. Identification and characterization of Methylobacterium dankookense sp. nov. isolated from drinking water. Master Thesis. Cheonan, Dankook University.
  10. Lee S, Chung HM, Park SJ, Choe B, Kim JH, Lee BR, et al. 2015. Identification and phylogenetic analysis of culturable bacteria in the bioareosol from several environments. Microbiol. Biotechnol. Lett. 43: 142–149. https://doi.org/10.4014/mbl.1503.03008
  11. Lee SA. 2003. Taxonomic study of denitrifying bacteria isolated from Daejeon sewage treatment plan. Master Thesis. Daejeon, Hannam University.
  12. Sánchez O, Ferrera I, González JM, Mas J. 2013. Assessing bacterial diversity in a seawater-processing wastewater treatment plant by 454-pyrosequencing of the 16S rRNA and amoA genes, Microb. Biotechnol. 6: 435−442. https://doi.org/10.1111/1751-7915.12052
  13. Tamaki H, Sekiguchi Y, Hanada S, Nakamura K, Nomura N, Matsumura M, et al. 2005. Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. Appl. Environ. Microbiol. 71: 2162−2169. https://doi.org/10.1128/AEM.71.4.2162-2169.2005
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731−2739. https://doi.org/10.1093/molbev/msr121
  15. Wang X, Hu M, Xia Y, Wen X, Ding K. 2012. Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China, Appl. Environ. Microbiol. 78: 7042−7047. https://doi.org/10.1128/AEM.01617-12