Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Current status and prospects of the authentication of Angelica species

Angelica 속 식물의 종판별을 위한 연구현황 및 전망

  • Gil, Jinsu (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Park, Sang ik (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Kim, Seong-Cheol (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Ok-Tae (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jung, Chan Sik (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Um, Yurry (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 길진수 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 박상익 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 이이 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 김호방 ((주)바이오메딕 생명과학연구소) ;
  • 김성철 (농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) ;
  • 김옥태 (농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) ;
  • 정찬식 (농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과) ;
  • 엄유리 (농촌진흥청 국립원예특작과학원 인삼특작부 약용작물과)
  • Received : 2016.04.19
  • Accepted : 2016.05.11
  • Published : 2016.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, natural cosmetics, and pharmaceutical industries. However, names are different from each country and their phylogenetic origins are not clear. These lead consumers to be confused. In particular, when they are morphologically similar and distributed as dried roots, it is extremely difficult to differentiate their origins even by specialists. Recently, molecular markers have been extensively applied to identify the origin of many crops. In this review, we tried to overview the current research achievements for the development of suitable 'origin identification' regarding to the differentiation of Angelica species. Furthermore, more advanced techniques including amplification genome based marker analyses are also discussed for their practical applications in the authentication of particular medicinal plant in Angelica species.

약용 식물 자원은 천연 화장품 소재, 제약 산업 그리고 인간의 건강을 위한 기능성 식품의 개발로 확대 된 이후 중요한 자산이 되고 있다. 그러나 세계적으로 약용 식물의 명칭을 각각 다르게 표기하고 있으며 계통 발생학적 기원이 명확하지 않다는 단점을 지니고 있다. 이 때문에 소비자들은 매우 큰 혼란을 겪고 있으며 특히 형태학적으로 유사한 식물의 말린 뿌리로 유통될 때에는 전문가들도 그들의 기원을 구별하기가 매우 어렵다. 이러한 이유때문에 이처럼 광범위하고 다양한 작물의 기원을 식별하기 위해 분자표지 기법을 적용하여 활용되고 있다. 이 리뷰에서 본 연구자들은 Angelica종의 분화에 관한 적합한 '기원정립'을 위한 현재의 연구 성과들을 정리했다. 결론적으로 Angelica종의 식별을 위해 개발된 분자적 연구에 대해 설명하고 약용작물의 유전체 정보를 활용하여 그들의 기원정립 및 판별에 대해 논의할 것이다.

Keywords

References

  1. Arano H, Saito H (1979) Cytological studies in family Umbelliferae. IV. Karyotypes in genus Angelica 2. La Kromosomo II-15-16:417-426
  2. Chung JW, Lee GA, Lee SS, Bang KH, Park CB, Park YJ (2009) Cultivar discrimination of Korean and Chinese boxthorn (Lycium chinense Mill. and Lycium barbarum L.) using SSR markers. Korean J Medicinal Crop Sci 17:445-451
  3. Bang KH, Chung JW, Kim YC, Lee JW, Jo IH, Seo AY, Kim OT, Hyun DY, Kim DH, Cha SW (2011a) Development of SSR markers for identification of Korean ginseng (Panax ginseng C. A. Meyer.) cultivars. Korean J Medicinal Crop Sci 19: 185-190 https://doi.org/10.7783/KJMCS.2011.19.3.185
  4. Bang KH, Jo IH, Chung JW, Kim YC, Lee JW, Seo AY, Park JH, Kim OT, Hyun DY, Kim DH and Cha SW (2011b) Analysis of genetic polymorphism of Korean ginseng cultivars and foreign accessions using SSR markers. Korean J Medicinal Crop Sci 19:347-353 https://doi.org/10.7783/KJMCS.2011.19.5.347
  5. Bang KH, Yu HS, Koo DH, Cho JH, Park HW, Seong NS, Park SI, Kim HS (2002) Selection of RAPD marker to discriminate the bolting-resistant varieties and commercial dried medicinal materials of Angelica species. Korean J Medicinal Crop Sci 10:46-50
  6. Boehm CL, Harrison HC, Jung G, Nienhuis J (1999) Organization of American and Asian ginseng germplasm using randomly amplified polymorphic DNA (RAPD) markers. J Am Soc Hortic Sci 124:252-256
  7. Chen S, Yao H, Han J, Liu C, Song J, Shi L, Zhu Y, Ma X, Gao T, Pang X, Luo K, Li Y, Li X, Jia X, Lin Y, Leon C (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5:1-8, e8613
  8. Cho CH, Kim SJ, Kim HJ (2002) Comparative studies on the discrimination of Angelicae Gigantis Radix by near-infrared spectroscopy, electronic nose and X-ray fluorescence spectrometry. Yakhak Hoeji 46:161-167
  9. Choi HW, Koo DH, Lee WK, Kim SY, Sung JS, Seong NS, Suh YB and Bang JW (2005) Cytogenetic analysis of seven Angelica species. Korean J Medicinal Crop Sci 13:118-121
  10. Choi HW, Song H, Koo DH, Bang JW, Hur Y (2007) Molecular and cytological characterization of species-specific repetitive DNA sequences for Angelica acutiloba. Korean J Genetic 29: 503-511
  11. Choi HY, Choi YJ, Lee JH, Ham Ih (2004) Sequencing analysis on the ITS region and AFLP analysis to identify dried medicinal Angelica species. Kor J Herbology 19:91-9
  12. Choo BK, Moon BC, Ji Y, Kim BB, Choi G, Yoon T, Kim HK (2009) Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva (Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer (ITS) sequence and random amplified polymorphic DNA (RAPD). Biol Pharm Bull 32:24-30 https://doi.org/10.1248/bpb.32.24
  13. Cui XM, Lo CK, Yip Kl, Dong TTX, Tsim KWK (2003) Authentification of Panax notoginseng by 5S-rRNA spacer domain and random amplified polymorphic DNA(RAPD) analysis. Planta Med 69:584-586 https://doi.org/10.1055/s-2003-40632
  14. Darlington CD, Wylie AP (1955) Chromesome atlas of flowering plants. The McMillan Co. New York, USA. pp.210
  15. Feng T, Liu S, He XJ (2010) Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA. Electron J Biotechn 13:9-10
  16. Gao WY, Qin EQ, Xiao XH, Yu HM, Gao GY, Chen SB, Zhao Y, Yang S (2001) Analysis on genuineness of Angelica sinensis by RAPD. Chinese Tradition Herbal Drugs 10:926-929
  17. Gupta SK, Bansal R, Gopalakrishna T (2014) Development and characterization of genic SSR markers for mungbean (Vigan radiate (L.) Wilczek). Euphytica 195:245-258 https://doi.org/10.1007/s10681-013-0993-0
  18. Hatano K, Nishioka I, Iwasa S (1975) Cytogenetical suties of Umbelliferous plants. III. The karyotype analysis of Angelica species in Japan. Jpn J Pharmacol 29:10-21
  19. He Y, Hou P, Fan G, Song Z, Liu H, Li Y, Zhang Y (2011) Internal transcribed spacers (ITS) identification of Angelica anomala Lallem Chuanbaizhi (in Chinese) cultivars collected in Sichuan and their molecular phylogenetic analysis with other Angelica L. species. J Med Plants Res 5:3653-3659
  20. Kim BB, Jeong JH, Jung SJ, Yun DW, Yoon ES, Choi YE (2005) Authentification of Korean Panax ginseng from Chinese Panax ginseng and Panax quinquefolius by AFLP analysis. J Plant Biotechnol 7:81-86
  21. Kim CK, Choi HK (2003) Genetic diversity and relationship in Korean ginseng (P. schinseng) based on RAPD analysis. Korean J Genetic 25:181-188
  22. Kim J, Jo BH, Lee KL, Yoon ES, Ryu GH, Chung KW (2007) Identification of new microsatellite markers in Panax ginseng. Mol Cells 24:60-68
  23. Kim K, Lee SC, Lee J, Lee HO, Joh HJ, Kim NH, Park HS, Yang TJ (2015) Comprehensive survey of genetic diversity in chloroplast genomes and 45S nrDNAs within Panax ginseng species. PloS ONE 10:e0117159 https://doi.org/10.1371/journal.pone.0117159
  24. Kim YH, Choi G, Lee HW, Lee GH, Chae SW, Kim YH, Lee MY (2012) Comparison of Angelica species roots using taste sensor and DNA sequencing analysis. Kor J Herbology 27:37-42
  25. Koo DH, Kim SY, Bang KH, Seong NS, Bang JW (2003) Cytogenetic analysis of Angelica plants using feulgen staining and multicolor fluorescence in situ hybridization. Kor J Plant Biotechnol 30:123-127 https://doi.org/10.5010/JPB.2003.30.2.123
  26. Lee MY, Im SH, Ju YS, Han KS, Jeong GJ, An DG, Kang HC, Ko BS (2000) Discrimination of the three Angelica species using the RAPDs and internal root structure. Korean J Medicinal Crop Sci Sci 8(3):243-249
  27. Lee MY, Ju YS, Kim HJ, Ko BS (2001) Discrimination of Aralia continentalis root by the random amplified polymorphic DNA analysis and morphological characteristics. Kor J Orient Med 7:145-152
  28. Lee SB, Rasmussen SK (2000) Molecular markers in some medicinal plants of the Apiaceae family. Euphytica 114:87-91 https://doi.org/10.1023/A:1003919416122
  29. Lee JW, Kim YC, Jo IH, Seo A, Lee JH, Kim OT, Hyun DY, Cha SW, Bang KH, Cho JH (2011) Development of an ISSRderived SCAR marker in Korean ginseng cultivars (Panax ginseng CA Meyer). J Ginseng Res 35:52-59 https://doi.org/10.5142/jgr.2011.35.1.052
  30. Liao C, Downie SR, Li Q, Yu Y, He X, Zhou B (2013) New insights into the phylogeny of Angelica and its allies (Apiaceae) with emphasis on east asian species, inferred from nrDNA, cpDNA, and Morphological Evidence. Syst Bot 38:266-281 https://doi.org/10.1600/036364413X662060
  31. Lobiuc A, Zamfirache MM, Ivanescu L (2012) Comparative anatomical investigations on some species of the genus Angelica L. Contributii Botanice 47
  32. Lu Y, Cheng T, Zhu T, Jiang D, Zhou S, Jin L, Yuan Q, Huang L (2015) Isolation and characterization of 18 polymorphic microsatellite markers for the "Female Ginseng" Angelica sinensis (Apiaceae) and cross-species amplification. Biochem Syst Ecol 61:488-492 https://doi.org/10.1016/j.bse.2015.07.013
  33. Mei Z, Zhang C, Khan A, Zhu Y, Tania M, Luo P, Fu J (2015) Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China. Electron J Biotechn 18:96-102 https://doi.org/10.1016/j.ejbt.2014.12.006
  34. Ministry of Agriculture, Food and Rural Affairs(MAFRA) (2015) Production performance of industrial crops. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea
  35. Mizukami H, Hao BS, Tanaka T (1997) Nucleotide sequence of 5S-rDNA intergenic spacer region on Angelica acutiloba. Nat Med 51:376-378
  36. Ngan F, Shaw P, But P, Wang J (1999) Molecular authentication of Panax species. Phytochemistry. 50:787-791 https://doi.org/10.1016/S0031-9422(98)00606-2
  37. Nguyen VD, Nirala R, Choi SR, Uhm TS, Yang TJ, Ahn IO, Lim YP (2010) Development and characterization of new microsatellite markers in Panax ginseng C. A. Meyer from BAC end sequences. Conserv Genet 11:1223-1225 https://doi.org/10.1007/s10592-009-9924-y
  38. Pan ZH, Chin HC, Wu ZJ, Yuan CQ (1985) The karyotype of Angelica dahurica and their taxonomical significance (Umbelliferae). Acta Phytotax Sin 23:185-187
  39. Sung JS, Bang KW, Park CH, Park CG, Yu HS, Park HW, Seong NS (2004) Discrimination of Angelica radix based on anatomical characters. Korean J Medicinal Crop Sci 12:67-72
  40. To CA (1970) Cytotaxonomical study of genus Angelica. J Kor Res Inst Better Living 5:57-61
  41. Tsukazaki H, Fukuoka H, Song YS, Yamashita K, Wako T, Kojima A (2006) Considerable heterogeneity in commercial F1 varieties of bunching onion (Allium wstulosum) and the proposal of a breeding scheme for conferring genetic traceability using SSR markers. Breeding Sci 56:321-326 https://doi.org/10.1270/jsbbs.56.321
  42. Um JY, Chung HS, Kim MS, Na HJ, Kwon HJ, Kim JJ, Lee KM, Lee SJ, Lim JP, Do KR, Hwang WJ, Lyu YS, An NH, Kim HM (2001) Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP. Biol Pharm Bull 24:872-875 https://doi.org/10.1248/bpb.24.872
  43. Vasil'eva MG, Pimenov MG (1991) Karyotaxomomical analysis in the genus Angelica (Umbelliferae). Plant Syst Evol 177:117-138 https://doi.org/10.1007/BF00937950
  44. Yang L, Wen C, Zh ao H, Kiu Q, Yang J, Liu L, Wang Y (2015) Development of polymorphic genic SSR Markers by transcriptome sequencing in the welsh onion (Allium fistulosum L.). Appl Sci 5:1050-1063 https://doi.org/10.3390/app5041050
  45. Yao H, Song J, Liu C, Luo K, Han J, Li Y, Pang X, Xu H, Zhu Y, Xiao P, Chen S (2010) Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS ONE 5:e13102 https://doi.org/10.1371/journal.pone.0013102
  46. Yu HS, Park CH, Park CG, Kim YG, Park HW, Seong NS (2004) Growth characteristics and tield of the three species of genus Angelica. Korean J Medicinal Crop Sci 12:43-46
  47. Zhao KJ, Dong TTX, Tu PF, Song ZH, Lo CK, Tsim KWK (2003) Molecular genetic and chemical assessment of radix Angelica (Danggui) in China. J Agr Food Chem 51:2576-25 https://doi.org/10.1021/jf026178h

Cited by

  1. Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation vol.24, pp.4, 2016, https://doi.org/10.7783/KJMCS.2016.24.4.317