DOI QR코드

DOI QR Code

고지방 식이로 비만이 유도된 C57BL/6 마우스에서 식이 기간에 따른 비만 관련 지표 변화에 대한 연구

The Study of the Changes of Obesity-Relating Biomarkers in High Fat Fed-Induced C57BL/6 Mice

  • 송미영 (동국대학교 한의과대학 한방재활의학교실)
  • Song, Mi-Young (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
  • 투고 : 2016.05.10
  • 심사 : 2016.06.09
  • 발행 : 2016.06.30

초록

Objectives: The prevalence of obesity continues rise and obesity and metabolic syndrome is a major problem in global health care. Animal models are used in the drug discovery of novel treatment for obesity. One of common models of obesity is a high fat diet induced obesity in a C5BL/6 mouse, and the development of obesity and glucose tolerance in mouse model is different according to period of diet. Therefore, this study was performed to observe the development of obesity and glucose tolerance during a high fat diet (HFD). Methods: Male C57BL/6 mice, 5 weeks of age, were fed on a standard chow diet as a normal diet (18 kcal% fat) or a HFD (60 kcal% fat) for up to 16 weeks. The various factors related with obesity and insulin resistance were measured at 8, 12, and 16 weeks. Results: The weights of body and epididymal fat were gradually increased for 8~16 weeks, however the change of hyperglycaemia and glucose tolerance have shown different with that of body weight. Blood glucose, oral glucose tolerance and insulin tolerance were increased more clearly at week 12 and 16 than week 8. Lipid accumulation of liver and body temperature were also significantly increased at week 16, compared with normal group. Conclusions: The developments of obesity and related factors were different by a HFD period in a C57BL/6 obese mice. This result suggests that the development of obesity with glucose tolerance and liver lipid may induce clearly by a HFD for 16 weeks.

키워드

참고문헌

  1. Korea Centers for Disease Control and Prevention. Korea National Health and Nutrition Examination Survey [Internet]. Cheongju: Korea Centers for Disease Control and Prevention; [cited 2016 May 9]. Available from: https://knhanes.cdc.go.kr/knhanes/index.do.
  2. Haslam DW, James WP. Obesity. Lancet. 2005 ; 366(9442) : 1197-209. https://doi.org/10.1016/S0140-6736(05)67483-1
  3. Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Invest. 2000 ; 106(4) : 473-81. https://doi.org/10.1172/JCI10842
  4. Jang SY. Guideline of herbal drug products for obesity treatment. Osong : Korea Food and Drug Administration. 2008 : 1-36.
  5. Lutz TA, Woods SC. Overview of animal models of obesity. Protoc Pharmacol. 2012 ; 5(61) : 1-22.
  6. Collins S, Martin TL, Surwit RS, Robidoux J. Genetic vulnerability to diet-induced obesity in the C57BL/6J mouse: physiological and molecular characteristics. Physiol Behav. 2004 ; 81(2) : 243-8. https://doi.org/10.1016/j.physbeh.2004.02.006
  7. Speakman J, Hambly C, Mitchell S, Krol E. Animal models of obesity. Obes Rev. 2007 ; 8(Suppl 1) : 55-61. https://doi.org/10.1111/j.1467-789X.2007.00319.x
  8. Sato A, Kawano H, Notsu T, Ohta M, Nakakuki M, Mizuguchi K, et al. Antiobesity effect of eicosapentaenoic acid in high-fat/high-sucrose diet-induced obesity: importance of hepatic lipogenesis. Diabetes. 2010 : 59(10) : 2495-504. https://doi.org/10.2337/db09-1554
  9. Wang CY, Liao JK. A mouse model of diet-induced obesity and insulin resistance methods. Mol Biol. 2012 ; 821 : 421-33.
  10. Aguiar AF, de Souza RW, Aguiar DH, Aguiar RC, Vechetti IJ Jr, Dal-Pai-Silva M. Creatine does not promote hypertrophy in skeletal muscle in supplemented compared with nonsupplemented rats subjected to a similar workload. Nutr Res. 2011 ; 31(8) : 652-7. https://doi.org/10.1016/j.nutres.2011.08.006
  11. Williams LM, Campbell FM, Drew JE, Koch C, Hoggard N, Rees WD, et al. The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases. PLoS One. 2014 ; 9(8) : e106159. https://doi.org/10.1371/journal.pone.0106159
  12. Liu Y, Dang H, Li D, Pang W, Hammock BD, Zhu Y. Inhibition of soluble epoxide hydrolase attenuates high-fat-diet-induced hepatic steatosis by reduced systemic inflammatory status in mice. PLoS One. 2012 ; 7(6) : e39165. https://doi.org/10.1371/journal.pone.0039165
  13. Chang HP, Wang ML, Chan MH, Chiu YS, Chen YH. Antiobesity activities of indole-3-carbinol in high-fat-diet-induced obese mice. Nutrition. 2011 ; 27(4) : 463-70. https://doi.org/10.1016/j.nut.2010.09.006
  14. Han CT, Lee SH, Lim SB, Kong HS, Kim SH, Lee SK, et al. Studies on production of high fat diet induced obesity C57BL/6NCrjBgi mice. Lab Anim Res. 2006 ; 22(3) : 221-6.
  15. Landsberg L, Young JB, Leonard WR, Linsenmeier RA, Turek FW. Is obesity associated with lower body temperatures? Core temperature: a forgotten variable in energy balance. Metabolism. 2009 ; 58(6) : 871-6. https://doi.org/10.1016/j.metabol.2009.02.017
  16. Jurgens HS, Schurmann A, Kluge R, Ortmann S, Klaus S, Joost HG, et al. Hyperphagia, lower body temperature, and reduced running wheel activity precede development of morbid obesity in New Zealand obese mice. Physiol Genom. 2006 ; 25(2) : 234-41. https://doi.org/10.1152/physiolgenomics.00252.2005
  17. Heikens MJ, Gorbach AM, Eden HS, Savastano DM, Chen KY, Skarulis MC, et al. Core body temperature in obesity. Am J Clin Nutr. 2011 ; 93(5) : 963-7. https://doi.org/10.3945/ajcn.110.006270
  18. Sishi B, Loos B, Ellis B, Smith W, du Toit EF, Engelbrecht AM. Diet-induced obesity alters signalling pathways and induces atrophy and apoptosis in skeletal muscle in a prediabetic rat model. Exp Physiol. 2011 ; 96(2) : 179-93. https://doi.org/10.1113/expphysiol.2010.054189
  19. Pompeani N, Rybalka E, Latchman H, Murphy RM, Croft K, Hayes A. Skeletal muscle atrophy in sedentary Zucker obese rats is not caused by calpain-mediated muscle damage or lipid peroxidation induced by oxidative stress. J Negat Results Biomed. 2014 ; 13 : 19. https://doi.org/10.1186/s12952-014-0019-z

피인용 문헌

  1. Anti-Diabetic Effects ofGlycine sojaExtract in Genetic Animal Model ofdb/dbMouse vol.16, pp.2, 2016, https://doi.org/10.15429/jkomor.2016.16.2.101
  2. 고지방식이로 유도된 비만형 동물모델에서 부자 물추출물의 비만 및 당대사 개선 효능 평가 vol.17, pp.1, 2016, https://doi.org/10.15429/jkomor.2017.17.1.29
  3. 고지방식이 비만모델에서 방기황기탕(防己黃芪湯) 합(合) 영계출감탕(苓桂朮甘湯)의 항비만 효과 vol.29, pp.4, 2019, https://doi.org/10.18325/jkmr.2019.29.4.29
  4. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2016, https://doi.org/10.15429/jkomor.2020.20.2.149