Biomolecules & Therapeutics

  • 제24권4호
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  • Pages.410-417
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  • 2016
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Differential Effects of Quercetin and Quercetin Glycosides on Human α7 Nicotinic Acetylcholine Receptor-Mediated Ion Currents

  • Lee, Byung-Hwan (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University) ;
  • Choi, Sun-Hye (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University) ;
  • Kim, Hyeon-Joong (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University) ;
  • Jung, Seok-Won (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University) ;
  • Hwang, Sung-Hee (Department of Pharmaceutical Engineering, Sangji University) ;
  • Pyo, Mi-Kyung (International Ginseng and Herb Research Institute) ;
  • Rhim, Hyewhon (Life Science Division, Korea Institute of Science and Technology) ;
  • Kim, Hyoung-Chun (Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University) ;
  • Kim, Ho-Kyoung (Mibyeong Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Sang-Mok (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University) ;
  • Nah, Seung-Yeol (Department of Physiology, College of Veterinary Medicine and BioMolecular Informatics Center, Konkuk University)
  • 투고 : 2015.09.21
  • 심사 : 2016.01.22
  • 발행 : 2016.07.01
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초록

Quercetin is a flavonoid usually found in fruits and vegetables. Aside from its antioxidative effects, quercetin, like other flavonoids, has a various neuropharmacological actions. Quercetin-3-O-rhamnoside (Rham1), quercetin-3-O-rutinoside (Rutin), and quercetin-3-(2(G)-rhamnosylrutinoside (Rham2) are mono-, di-, and tri-glycosylated forms of quercetin, respectively. In a previous study, we showed that quercetin can enhance ${\alpha}7$ nicotinic acetylcholine receptor (${\alpha}7$ nAChR)-mediated ion currents. However, the role of the carbohydrates attached to quercetin in the regulation of ${\alpha}7$ nAChR channel activity has not been determined. In the present study, we investigated the effects of quercetin glycosides on the acetylcholine induced peak inward current ($I_{ACh}$) in Xenopus oocytes expressing the ${\alpha}7$ nAChR. $I_{ACh}$ was measured with a two-electrode voltage clamp technique. In oocytes injected with ${\alpha}7$ nAChR copy RNA, quercetin enhanced $I_{ACh}$, whereas quercetin glycosides inhibited $I_{ACh}$. Quercetin glycosides mediated an inhibition of $I_{ACh}$, which increased when they were pre-applied and the inhibitory effects were concentration dependent. The order of $I_{ACh}$ inhibition by quercetin glycosides was Rutin${\geq}$Rham1>Rham2. Quercetin glycosides-mediated $I_{ACh}$ enhancement was not affected by ACh concentration and appeared voltage-independent. Furthermore, quercetin-mediated $I_{ACh}$ inhibition can be attenuated when quercetin is co-applied with Rham1 and Rutin, indicating that quercetin glycosides could interfere with quercetin-mediated ${\alpha}7$ nAChR regulation and that the number of carbohydrates in the quercetin glycoside plays a key role in the interruption of quercetin action. These results show that quercetin and quercetin glycosides regulate the ${\alpha}7$ nAChR in a differential manner.

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참고문헌

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