Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways

  • Yang, Ming (Department of Periodontology, School of Dentistry, Chonbuk National University) ;
  • Li, Liang (Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University) ;
  • Heo, Seok-Mo (Department of Periodontology, School of Dentistry, Chonbuk National University) ;
  • Soh, Yunjo (Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University)
  • Received : 2016.01.28
  • Accepted : 2016.05.24
  • Published : 2016.07.01
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Abstract

Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders.

Keywords

References

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