The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Nivalenol-Producing Fusarium culmorum Isolates Obtained from the Air at a Rice Paddy Field in Korea

  • Kim, Da-Woon (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Kim, Gi-Yong (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Kim, Hee-Kyoung (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Kim, Jueun (Department of Chemistry, Chonnam National University) ;
  • Jeon, Sun Jeong (Division of Food Technology, Biotechnology & Agrochemistry, Chonnam National University) ;
  • Lee, Chul Won (Department of Chemistry, Chonnam National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, Chonnam National University) ;
  • Yun, Sung-Hwan (Department of Medical Biotechnology, Soonchunhyang University)
  • Received : 2015.12.18
  • Accepted : 2016.02.18
  • Published : 2016.06.01
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Abstract

Together with the Fusarium graminearum species complex, F. culmorum is a major member of the causal agents of Fusarium head blight on cereals such as wheat, barley and corn. It causes significant yield and quality losses and results in the contamination of grain with mycotoxins that are harmful to humans and animals. In Korea, F. culmorum is listed as a quarantine fungal species since it has yet to be found in the country. In this paper, we report that two isolates (J1 and J2) of F. culmorum were collected from the air at a rice paddy field in Korea. Species identification was confirmed by phylogenetic analysis using multilocus sequence data derived from five genes encoding translation elongation factor, histone H3, phosphate permease, a reductase, and an ammonia ligase and by morphological comparison with reference strains. Both diagnostic PCR and chemical analysis confirmed that these F. culmorum isolates had the capacity to produce nivalenol, the trichothecene mycotoxin, in rice substrate. In addition, both isolates were pathogenic on wheat heads and corn stalks. This is the first report on the occurrence of F. culmorum in Korea.

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