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Secondary structure analysis of MRA1997 from Mycobacterium tuberculosis and characterization of DNA binding property

  • Kim, Hyo Jung (Research Institute of Pharmaceutical sciences, College of Pharmacy, Seoul National University) ;
  • Lee, Ki-Young (Research Institute of Pharmaceutical sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Yena (Research Institute of Pharmaceutical sciences, College of Pharmacy, Seoul National University) ;
  • Kwon, Ae-Ran (Department of Herbal Skin Care, College of Herbal Bio-industry, Daegu Haany University) ;
  • Lee, Bong-Jin (Research Institute of Pharmaceutical sciences, College of Pharmacy, Seoul National University)
  • 투고 : 2016.03.19
  • 심사 : 2016.05.07
  • 발행 : 2016.06.20

초록

MRA1997 is a highly conserved protein from mycobacterial strains. However, no structural and functional information is associated with it. Thus, to obtain details about structure and function of this protein, we have utilized NMR spectroscopy. The recombinant MRA1997 was highly purified and its DNA binding mode was characterized. The tertiary structure of MRA1997 was modeled on the basis of our NMR chemical shift data combined with the webserver CS23D. The binding of MRA1997 with DNA was first monitored by electrophoresis mobility shift assays. The residues involved in DNA binding are identified using NMR chemical shift perturbation experiments. Based on our study, we suggest that MRA1997 interacts with DNA and may play an important role in Mycobacterium tuberculosis physiology.

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참고문헌

  1. P. B. Pavlinac, Int. J. Tuverc. Lung Dis. 20, 895 (2016) https://doi.org/10.5588/ijtld.15.0773
  2. F. A. Krsulovic, PloS one 11, e0153710 (2016) https://doi.org/10.1371/journal.pone.0153710
  3. R. H. Chisholm, The Proceedings of The Royal Society 283, (2016)
  4. A. Jain, Int. J. Tuverc. Lung Dis. 20, 870 (2016) https://doi.org/10.5588/ijtld.15.0874
  5. J. Tang, Tuberculosis 98, 30 (2016) https://doi.org/10.1016/j.tube.2016.02.005
  6. J. W. Wilson, Mayo Clinic proceedings 91, 482 (2016) https://doi.org/10.1016/j.mayocp.2016.01.014
  7. H. J. Kim, Journal of the Korean Magnetic Resonance Society 19, 49 (2015) https://doi.org/10.6564/JKMRS.2015.19.1.049
  8. F. Delaglio, Journal of biomolecular NMR 6, 277 (1995)
  9. B. A. Johnson, Methods in molecular biology 278, 313 (2004)
  10. D. S. Wishart, Nucleic acids research 36, 496 (2008) https://doi.org/10.1093/nar/gkn305
  11. L, N. Shen, The Biochemical Journal 397, 279 (2006) https://doi.org/10.1042/BJ20052030
  12. X. Robert, Nucleic acids research 42, W320
  13. P. Gouet, Bioinformatics 15, 305
  14. E. F. Patterson, J. Comput. Chem. 25, 1605 (2004) https://doi.org/10.1002/jcc.20084