The Korean Journal of Community Living Science (한국지역사회생활과학회지)

The Korean Society of Community Living Science (한국지역사회생활과학회)
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Effects of Loquat (Eriobotrya japonica Lindl.) Ethanol Extracts of Different Aerial Parts on Antioxidant Activity and Antiproliferation of Human Cancer Cells

비파 부위별 에탄올 추출물의 항산화 활성 및 암세포 증식 억제효과

  • Lee, Hwan (Dept. of Food and Nutrition, Chosun University) ;
  • Kim, Yeon-Kyoung (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Hyun-Joo (Dept. of Nutrition and Culinary Science, Hankyoung National University) ;
  • Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
  • 이환 (조선대학교 식품영양학과) ;
  • 김연경 (조선대학교 식품영양학과) ;
  • 이현주 (한경대학교 영양조리과학과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2016.03.03
  • Accepted : 2016.05.26
  • Published : 2016.05.31
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Abstract

The current study was carried out to determine the effects of the seed, flesh (seedless fruit), and leaf of loquat (Eriobotrya japonica Lindle.) on antioxidative activity and anti-proliferation in human cancer cells. Total polyphenol contents of loquat seed, flesh, and leaf ethanol extracts were found to be 17.77, 32.32, and 28.08 mg/g, respectively. Also, total flavonoid contents of loquat seed, flesh, and leaf ethanol extracts were found to be 18.77, 28.73, and 21.35 mg/g, respectively. The $IC_{50}$ values of DPPH hydroxyl scavenging of loquat seed, flesh, and leaf ethanol extracts were 0.049, 0.063, and 0.042 mg/mL, respectively. Antioxidative indexes of loquat leaf and seed ethanol extracts was highly and it was similar to the BHA and BHT. The antioxidative activities in loquat seed and leaf ethanol extracts were higher in loquat flesh. The antiproliferation effect of loquat seed and leaf ethanol extracts on liver cancer cell line (H460), stomach cancer cell line (AGS) and lung cancer cell line (A549) showed higher values compared with the flesh ethanol extracts. These results indicate that loquat ethanol extracts may play a positive role in antioxidative properties and cancer prevention.

본 연구는 비파 씨, 과육 및 잎의 부위별 에탄올 추출물이 항산화효과 및 암세포 증식 억제효과를 알아보고자 실시하였다. 총 폴리페놀과 총 플라보노이드 함량은 비파 잎 에탄올 추출물에서 가장 높게 나타났으며, 다음으로는 비파 씨, 과육 추출물 순으로 나타났다. 비파 씨, 과육 및 잎 추출물의 DPPH radical 소거능의 $IC_{50}$값은 0.049, 0.063, 0.042 mg/mL로 비파 씨와 잎이 과육에 비하여 항산화 활성이 좋은 것으로 나타났다. Rancimat에 의한 항산화능 측정 결과도 비파 씨와 잎 추출물이 높게 나타났다. 비파 부위별 추출물의 암세포 성장억제 효과를 측정한 결과, 비파 씨 에탄올 추출물은 위암과 폐암세포는 농도 의존적으로 저하시켰으나, 간암세포의 경우는 $400{\mu}g/mL$부터 유의하게 저하시켰다. 비파 잎과 과육 에탄올 추출물은 고농도에서 암세포 증식 억제효과가 나타났다. 이상의 결과 비파 부위별 에탄올 추출물의 항산화 및 암세포 증식 억제효과는 모든 부위에서 나타났으며, 특히 비파 씨 추출물이 가장 좋은 결과를 보였다.

Keywords

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